The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN 14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN 14, containing divergently orientated P(prtP) and P(prtP) promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of P(prtM) in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas P(prtP) was completely inactive. When L. casei ATCC393(T) was used as host, both P(prtP) and P(prtM) were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.

• MeSH
• elektroforéza v polyakrylamidovém gelu využití   MeSH
• financování organizované využití   MeSH
• kaseiny genetika   MeSH
• Lactobacillus genetika   růst a vývoj   MeSH
• plazmidy genetika   MeSH
• polymerázová řetězová reakce využití   MeSH
• promotorové oblasti (genetika) imunologie   účinky záření   MeSH
• regulace genové exprese imunologie   účinky záření   MeSH
• sekvenční analýza metody   využití   MeSH