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34 záznamů v Medvik Filtry

Abstrakt

Účinky borneolu, bicyklického terpénu, na cicavčie bunky rôzneho pôvodu : Praha, Lékařský dům, 11.-13. červen 2007 [abstrakt]

12. mezioborová česko-slovenská toxikologická konference. 2007 ; () : s. 39.

ISSN 978-80-7071-283-2
Medvik
Zdroj

Abstrakt

Effects of borneol, a bicyclic terpene, on human cells of different origin

Horváthová, Eva
Autor Autorita Laboratory of Mutagenesis and Carcinogenesis, Cancer Research Institute, Slovak Academy of Sciences, Vlárska 7, 833 91 Bratislava, Slovakia
Slameňová, Darina
Autor Slameňová, Darina Laboratory of Mutagenesis and Carcinogenesis, Cancer Research Institute, Slovak Academy of Sciences, Vlárska 7, 833 91 Bratislava, Slovakia

Chemické listy. 2007 ; 101 (S) : s107-s109. (12. mezioborová česko-slovenská toxikologická konference. Praha, Lékařský dům, 11.-13. června 2007)

Chem. Listy
ISSN 0009-2770
Medvik
Zdroj

Soubor příspěvků ústních a plakátových sdělení z 12. mezioborové česko-slovenské toxikologické konference. 2007 ; 101 (S) : s107-s109.

ISSN 0009-2770
Medvik
Zdroj

Článek

Genotoxicity of environmental air pollution in three European cities: Prague, Kosice and Sofia

Mutation research. 2004 ; 563 (1) : 49-59. [pub] 2004Sep12

Mutat Res
ISSN 0027-5107
Medvik
Zdroj

The genotoxic potential of extractable organic matter (EOM) associated with the respirable particulate matter (PM <10 microm) of atmospheric pollution has been determined in three European cities--Prague (Czech Republic, two monitoring sites, Libus and Smíchov), Kosice (Slovak Republic) and Sofia (Bulgaria) using the alkaline single-cell gel electrophoresis (the comet assay). The EOM samples were extracted by dichloromethane from ambient airborne particles collected daily (24 h intervals) during 3-month sampling periods in winter and summer seasons. The human metabolically competent cell line Hep G2 was used as a test system and benzo[a]pyrene (BaP), a known carcinogen, was applied as a positive control (internal standard) in each electrophoretic run. Two-hour exposure of Hep G2 cells to equivalent EOM concentrations ranging from 5 to 150 microg EOM/ml resulted in a linear dose-dependent increase of DNA migration (r > 0.9, P < 0.01). A less significant dose-response (r = 0.61) was only induced by the EOM sample from the locality Prague-Libus (PRG-LB) in the winter. Generally, a 1.5 to four-fold increase of DNA strand breaks over the background control level was determined in EOM-exposed cells. In order to compare the genotoxic potential of individual EOMs, a mathematical model was used to correct the 'real' data. No substantial location- or season-related differences were found in EOM genotoxicity (EOM microg/ml), except for the EOM sample from Sofia, collected in the summer. This EOM sample induced a nearly two-fold lower level of DNA damage in comparison with other EOMs. On the other hand, clear statistically significant location- and season-related differences (P < 0.001) in ambient air genotoxicity were determined when the EOM quantity per cubic meter of air (microg/m3) was taken into account. In that case, the genotoxicity of winter air pollution was six- to 10-fold higher in comparison with summer air. The air pollution genotoxicity in individual localities rose during the winter season in the order: PRG-LB < Kosice < Prague-Smíchov (PRG-SM) < Sofia, while during the summer season the highest ambient air genotoxicity was revealed in the locality Prague-Smíchov and approximately equal air pollution genotoxicity was determined among localities Prague-Libus, Kosice and Sofia (PRG-LB approximately Kosice approximately Sofia < PRG-SM). The greatest overall air pollution genotoxicity was determined in the locality Sofia during the winter season. In a time course study to evaluate the kinetics of DNA strand break rejoining it was shown that the level of DNA strand breaks in EOM-exposed cells has returned to near the background level within 24 h after the treatment.