- MeSH
- akademie a ústavy MeSH
- bakteriociny MeSH
- biologie výchova MeSH
- laboratoře MeSH
- výzkum MeSH
- Publikační typ
- biografie MeSH
- rozhovory MeSH
- O autorovi
- Šmarda, Jan, 1930-2021 Autorita
3. přeprac. vyd. 131 s. ; 30 cm
- Konspekt
- Přírodní vědy. Matematické vědy
- NLK Obory
- fyzika, biofyzika
- chemie, klinická chemie
- biologie
- NLK Publikační typ
- testy
The P19 mouse embryonal carcinoma cell line was used as a model for a study of apoptosis accompanying differentiation induced by all-trans retinoic acid (ATRA). Apoptosis was detected both on the basis of morphological features (nuclear fragmentation, blebbing of plasma membrane, and formation of apoptotic bodies), and by using DNA electrophoresis and flow-cytometric measurement of DNA content. Actin cytoskeleton was studied both on morphological and submicroscopic levels. ATRA-treated cells manifested apoptosis-specific changes in the distribution of actin foremost in association with their entry into executive phase of apoptosis, when F-actin cables participated in cell disintegration into apoptotic bodies. Using immunogold labeling, actin was also identified in centers of fragmenting apoptotic nuclei, in the disintegration of which it is likely involved as well. At the same time, a cleavage of actin by active caspase-3 was proved, resulting in the emergence of 32 kDa fragment, termed fractin. Measurement of F-actin and fractin content using flow cytometry showed an unequivocal decrease of F-actin and synchronous increase of fractin in the apoptotic population as compared to non-treated cells. Therefore, our results proved both actin proteolysis and active involvement of specific actin structures in the final cell disintegration during apoptosis in the P19 cells.
- MeSH
- aktiny fyziologie chemie metabolismus MeSH
- apoptóza MeSH
- buněčná diferenciace MeSH
- buněčné jádro metabolismus MeSH
- cytoskelet metabolismus MeSH
- DNA metabolismus MeSH
- elektroforéza MeSH
- embryonální karcinom metabolismus MeSH
- financování organizované MeSH
- fluorescenční mikroskopie MeSH
- fluorescenční protilátková technika nepřímá MeSH
- fragmentace DNA MeSH
- HL-60 buňky MeSH
- imunohistochemie MeSH
- kaspasy metabolismus MeSH
- lidé MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- peptidové fragmenty fyziologie chemie MeSH
- průtoková cytometrie MeSH
- radiační rozptyl MeSH
- světlo MeSH
- transmisní elektronová mikroskopie MeSH
- tretinoin metabolismus MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- abstrakt z konference MeSH
