This study was undertaken to investigate the starter and probiotic potential of lactic acid bacteria isolated from dromedarian camel's milk using both culture-dependent and -independent approaches and metataxonomic analysis. Strains of lactic acid bacteria recovered were examined in vitro for tolerance to gastric acidity, bile, and lysozyme. Bile salt hydrolysis, serum cholesterol-lowering, oxalate degradation, proteolytic activity, exopolysaccharide production, and cell surface characteristics necessary for colonizing intestinal mucosa were also evaluated. A single strain of the species, Lactobacillus fermentum named NPL280, was selected through multivariate analysis as it harbored potential probiotic advantages and fulfilled safety criteria. The strain assimilated cholesterol, degraded oxalate, produced exopolysaccharides, and proved to be a proficient alternate yogurt starter with good viability in stored bio-yogurt. A sensorial analysis of the prepared bio-yogurt was also found to be exemplary. We conclude that the indigenous L. fermentum strain NPL280 has the desired traits of a starter and adjunct probiotic culture for dairy products.
- MeSH
- jogurt mikrobiologie MeSH
- Limosilactobacillus fermentum * metabolismus MeSH
- mikrobiota fyziologie MeSH
- mléko * mikrobiologie MeSH
- probiotika * klasifikace izolace a purifikace metabolismus MeSH
- velbloudi MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Pákistán MeSH
In order to study the damaging or beneficial properties of bromine atom-free radical, reaction of the free radical (Br•) with some biologically important compounds were investigated. Br• was generated through electrochemical oxidation of bromide ion (Br–). First the reactivity of Br• atom-free radical vis a vis its dimerization to form Br2, was studied using cyclic voltammetry and spectroelectrochemistry. Through these techniques it was ascertained that the substrates understudy and the under experimental conditions used, underwent reactions with Br• and not with dibromine (Br2). The monitoring of the reactions of Br• with glycine and cytosine led us to conclude that whereas cytosine reacted with Br• as simple chemical reaction (EC mechanism), glycine underwent a catalytic reaction (EC' mechanism).
