Cryptococcus laurentii strain AL(27) demonstrating significant potential for intracellular phytase production was selected by 2-step screening of Antarctic yeasts. The strain showed increased phytase activity in a culture medium with 40 g/L sucrose, KH(2)PO(4) providing 5 mg/L phosphorus, and cultivation temperature of 24 degrees C, which relates it to psychrotrophic microorganisms. The enzyme kinetic characteristics according to sodium phytate were K (m) = 0.98 mmol/L, v (lim) = 33.3 mumol g(-1) min(-1). The enzyme had maximum activity at 40 degrees C and acted within a wide pH range: from 2.0 to 5.5, which is of positive significance for its direct inclusion into the feed of monogastric animals.
- MeSH
- Cryptococcus izolace a purifikace patogenita MeSH
- dospělí MeSH
- meningitida MeSH
- plicní mykózy MeSH
- tuberkulóza komplikace MeSH
- Check Tag
- dospělí MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- MeSH
- Columbidae MeSH
- Cryptococcus izolace a purifikace MeSH
- drůbež MeSH
- feces chemie MeSH
- ptáci MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Geografické názvy
- Československo MeSH
