Heat Shock Protein 90 (HSP90) is a fertility-associated protein, the expression of which positively correlates with sperm quality in many species. Geranylgeranylacetone (GGA) is reported to induce expression of HSP90. The present study aimed to investigate whether GGA induced expression of HSP90 in Acipenser sinensis sperm to exert a cryoprotective effect. Sperm from five male A. sinensis was combined with extender containing 20 mmol/L tris pH = 8.1, 10% v/v methanol, 2-5 mmol/L KCl, 15 mmol/L lactose, and 15 mmol/L trehalose, with GGA at 0, 14, 67, 135, 673, 1346, or 6731 μmol/L. After cryopreservation and thawing, the percentage of motile spermatozoa, spermatozoon curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), acrosome integrity, and membrane integrity, as well as fertility were evaluated. Sperm quality increased with the increase of GGA to 673 μmol/L, but decreased at higher concentrations. Expression levels of HSP90α were detected by Western blot in sperm frozen with GGA at 673 μmol/L (highest obtained sperm quality), 6731 μmol/L (highest GGA concentration), and a control without GGA. The expression of HSP90α increased with the increase in GGA, with lowest expression observed in the control. GGA was found to induce increase of HSP90α, and this increase was associated with higher quality cryopreserved sperm at concentrations ≤673 μmol/L. This research suggests a viable technique to increase the quality of cryopreserved A. sinensis sperm by adding GGA to induce expression of HSP90α.

• MeSH
• Cytoprotection * drug effects   genetics   MeSH
• Diterpenes pharmacology   MeSH
• Enzyme Induction drug effects   MeSH
• Cryopreservation veterinary   MeSH
• Cryoprotective Agents metabolism   pharmacology   MeSH
• Sperm Motility genetics   MeSH
• HSP90 Heat-Shock Proteins genetics   metabolism   MeSH
• Fishes * genetics   metabolism   MeSH
• Spermatozoa MeSH
• Semen Preservation * veterinary   MeSH
• Animals MeSH
• Check Tag
• Male MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH

Neisseria meningitidis colonizes the human nasopharynx and occasionally causes lethal or damaging septicemia and meningitis. Here, we examined the adherence-mediated signaling of meningococci to human cells by comparing gene expression profiles of human umbilical vein endothelial cells (HUVEC) infected by adherent wild-type, frpC-deficient mutant, or the nonadherent (DeltapilD) N. meningitidis. Pili-mediated adhesion of meningococci resulted in alterations of expression levels of human genes known to regulate apoptosis, cell proliferation, inflammatory response, adhesion and genes for signaling pathway proteins such as TGF-beta/Smad, Wnt/beta-catenin and Notch/Jagged. This reveals that adhering piliated meningocci manipulate host signaling pathways controlling cell proliferation while establishing a commensal relationship.

• MeSH
• Apoptosis immunology   MeSH
• Bacterial Adhesion MeSH
• Bacterial Proteins genetics   MeSH
• Cell Culture Techniques MeSH
• Cytoprotection genetics   MeSH
• Embryo, Mammalian metabolism   MeSH
• Endothelial Cells microbiology   MeSH
• Financing, Organized MeSH
• Humans MeSH
• Membrane Proteins genetics   deficiency   MeSH
• Neisseria meningitidis pathogenicity   MeSH
• Fimbriae Proteins genetics   deficiency   MeSH
• Flow Cytometry MeSH
• RNA, Complementary MeSH
• Oligonucleotide Array Sequence Analysis MeSH
• Signal Transduction MeSH
• Gene Expression Profiling MeSH
• Up-Regulation MeSH
• Umbilical Veins cytology   MeSH
• Inflammation genetics   MeSH
• Check Tag
• Humans MeSH

• MeSH
• Cytoprotection genetics   immunology   MeSH
• Gene Expression genetics   immunology   MeSH
• Research Support as Topic MeSH
• Genes, p53 genetics   immunology   MeSH
• Humans MeSH
• RNA, Small Interfering genetics   immunology   isolation & purification   MeSH
• Mutation genetics   MeSH
• RNA Interference immunology   MeSH
• Genes, Tumor Suppressor MeSH
• Gene Silencing immunology   MeSH
• Check Tag
• Humans MeSH