HotSpot Wizard is a web server for automatic identification of 'hot spots' for engineering of substrate specificity, activity or enantioselectivity of enzymes and for annotation of protein structures. The web server implements the protein engineering protocol, which targets evolutionarily variable amino acid positions located in the active site or lining the access tunnels. The 'hot spots' for mutagenesis are selected through the integration of structural, functional and evolutionary information obtained from: (i) the databases RCSB PDB, UniProt, PDBSWS, Catalytic Site Atlas and nr NCBI and (ii) the tools CASTp, CAVER, BLAST, CD-HIT, MUSCLE and Rate4Site. The protein structure and e-mail address are the only obligatory inputs for the calculation. In the output, HotSpot Wizard lists annotated residues ordered by estimated mutability. The results of the analysis are mapped on the enzyme structure and visualized in the web browser using Jmol. The HotSpot Wizard server should be useful for protein engineers interested in exploring the structure of their favourite protein and for the design of mutations in site-directed mutagenesis and focused directed evolution experiments. HotSpot Wizard is available at http://loschmidt.chemi.muni.cz/hotspotwizard/.
- MeSH
- beta-laktamasy chemie MeSH
- glykosidhydrolasy chemie MeSH
- hydrolasy triesterů kyseliny fosforečné chemie MeSH
- hydrolasy chemie MeSH
- internet MeSH
- proteinové inženýrství MeSH
- reprodukovatelnost výsledků MeSH
- software MeSH
- uživatelské rozhraní počítače MeSH
- Publikační typ
- práce podpořená grantem MeSH
A 2-month survey of extended-spectrum beta-lactamase (ESBL) producers was performed in a Czech hospital. Klebsiella pneumoniae produced SHV-2, -5, or -12, Escherichia coli produced CTX-M-9 or -15, and other species produced TEM-92 or -132. All K. pneumoniae and E. coli isolates belonged to sequence types (STs) or clonal complexes (CCs) spread across the world (K. pneumoniae clonal complex 11 [CC11], CC14, and sequence type 101 [ST101] and E. coli CC31, CC73, CC131, and CC405) and carried various plasmids (mainly with A/C- and FII-type replicons).
- MeSH
- antibakteriální látky farmakologie MeSH
- beta-laktamasy biosyntéza genetika chemie metabolismus MeSH
- DNA bakterií genetika chemie MeSH
- enterobakteriální infekce mikrobiologie MeSH
- Escherichia coli enzymologie izolace a purifikace klasifikace MeSH
- genotyp MeSH
- Klebsiella pneumoniae enzymologie izolace a purifikace klasifikace MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- nemocnice MeSH
- plazmidy MeSH
- techniky typizace bakterií MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- antibakteriální látky farmakologie chemie klasifikace MeSH
- beta-laktamasy antagonisté a inhibitory chemie terapeutické užití MeSH
- cefalosporiny chemie terapeutické užití MeSH
- karbapenemy terapeutické užití MeSH
- kyselina klavulanová terapeutické užití MeSH
- laktamy MeSH
- peniciliny terapeutické užití MeSH
- Publikační typ
- přehledy MeSH
