In hemolymph of insect species, compounds with remarkable properties for pharmaceutical industry are present. At the first line, there were found compounds of low molecular mass, less than 1 kDa. One of such compounds, β-alanyl-tyrosine (252 Da), was isolated from larval hemolymph of some species of holometabolous insects (e.g. Neobellieria bullata). Its paralytic activity and antimicrobial properties were described until now. In this study, we present the effect of elongation of β-alanyl-tyrosine by repeating of this motive on the biological and physical properties of prepared analogues. For assessment of antimicrobial properties of these new compounds strains of Gram-positive, Gram-negative bacteria and fungi were used, we also followed the haemolytic activity and toxic effect on human cell culture HepG2. On the base of ECD spectroscopy measurement, subsequent molecular modelling and known secondary structure of original β-alanyl-tyrosine dipeptide, the secondary structures of repeating sequences of β-AY were specified. The repeating structures of β-alanyl-tyrosine show increase in antimicrobial activity; for Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, minimal inhibitory concentration was decreased from 30 to 15 mM for 2xβ-AY, 0.4 mM for 4xβ-AY and 0.25 mM for 6xβ-AY.
- MeSH
- aminokyselinové motivy MeSH
- antiinfekční látky chemie farmakologie MeSH
- biologické toxiny chemie farmakologie MeSH
- buňky Hep G2 MeSH
- dipeptidy chemie farmakologie MeSH
- houby účinky léků MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- proliferace buněk účinky léků MeSH
- Staphylococcus aureus účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- alkaloidy terapeutické užití MeSH
- anabasin analogy a deriváty terapeutické užití MeSH
- bicyklické sloučeniny heterocyklické terapeutické užití MeSH
- biologické toxiny farmakokinetika farmakologie terapeutické užití MeSH
- biomedicínský výzkum MeSH
- psychofarmakologie MeSH
- pyridiny terapeutické užití MeSH
4th ed. 148 s. : obr., tab., grafy ; 22 cm
