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Článek

Analysis of the characteristics of phosphine production by anaerobic digestion based on microbial community dynamics, metabolic pathways, and isolation of the phosphate-reducing strain

Fan, Yimin
Autor Fan, Yimin School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China. Electronic address: 2725610181@qq.com
Niu, Xiaojun
Autor Niu, Xiaojun School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, China Sino-Singapore International Joint Research Institute, Guangzhou, 510700, PR China Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 510006, PR China The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 510006, PR China. Electronic address: xjniu@scut.edu.cn
Zhang, Dongqing
Autor Zhang, Dongqing School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, China. Electronic address: dqzhang3377@outlook.com
Lin, Zhang
Autor Lin, Zhang School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China. Electronic address: zlin@scut.edu.cn
Fu, Mingli
Autor Fu, Mingli School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China. Electronic address: mlfu@scut.edu.cn
Zhou, Shaoqi
Autor Zhou, Shaoqi School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China. Electronic address: 2975742087@qq.com

Chemosphere. 2021 ; 262 (-) : 128213. [pub] 20200903

ISSN 1879-1298
Medvik
Zdroj

Although phosphine is ubiquitously present in anaerobic environments, little is known regarding the microbial community dynamics and metabolic pathways associated with phosphine formation in an anaerobic digestion system. This study investigated the production of phosphine in anaerobic digestion, with results indicating that phosphine production mainly occurred during logarithmic microbial growth. Dehydrogenase and hydrogen promoted the production of phosphine, with a maximum phosphine concentration of 300 mg/m3. The abundance of Ruminococcaceae and Escherichia was observed to promote phosphine generation. The analysis of metabolic pathways based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the MetaCyc pathway database revealed the highest relative abundance of replication and repair in genetic information processing; further, the cofactor, prosthetic group, electron carrier, and vitamin biosynthesis were observed to be closely related to phosphine formation. A phylogenetic tree was reconstructed based on the neighbor-joining method. The results indicated the clear evolutionary position of the isolated Pseudescherichia sp. SFM4 strain, adjacent to Escherichia, with a stable phosphate-reducing ability for a maximum phosphine concentration of 26 mg/m3. The response surface experiment indicated that the initial optimal conditions for phosphine production by SFM4 could be achieved with nitrogen, carbon, and phosphorus loads of 6.17, 300, and 10 mg/L, respectively, at pH 7.47. These results provide comprehensive insights into the dynamic changes in the microbial structure, isolated single bacterial strain, and metabolic pathways associated with phosphine formation. They also provide information on the molecular biology associated with phosphorus recycling.