Bradykinin can enhance skeletal muscle glucose uptake (GU), and exercise increases both bradykinin production and muscle insulin sensitivity, but bradykinin's relationship with post-exercise insulin action is uncertain. Our primary aim was to determine if the B2 receptor of bradykinin (B2R) is essential for the post-exercise increase in GU by insulin-stimulated mouse soleus muscles. Wildtype (WT) and B2R knockout (B2RKO) mice were sedentary or performed 60 minutes of treadmill exercise. Isolated soleus muscles were incubated with [(3)H]-2-deoxyglucose +/-insulin (60 or 100 microU/ml). GU tended to be greater for WT vs. B2RKO soleus with 60 microU/ml insulin (P=0.166) and was significantly greater for muscles with 100 microU/ml insulin (P<0.05). Both genotypes had significant exercise-induced reductions (P<0.05) in glycemia and insulinemia, and the decrements for glucose (~14 %) and insulin (~55 %) were similar between genotypes. GU tended to be greater for exercised vs. sedentary soleus with 60 microU/ml insulin (P=0.063) and was significantly greater for muscles with 100 microU/ml insulin (P<0.05). There were no significant interactions between genotype and exercise for blood glucose, plasma insulin or GU. These results indicate that the B2R is not essential for the exercise-induced decrements in blood glucose or plasma insulin or for the post-exercise increase in GU by insulin-stimulated mouse soleus muscle.
- MeSH
- genotyp MeSH
- inzulin lidský farmakologie krev metabolismus MeSH
- kondiční příprava zvířat MeSH
- kosterní svaly metabolismus MeSH
- krevní glukóza metabolismus MeSH
- lidé MeSH
- myši knockoutované MeSH
- myši MeSH
- receptor bradykininu B2 genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Klíčová slova
- ketogeneze,
- MeSH
- diabetes mellitus krev MeSH
- hydroxybutyráty analýza krev MeSH
- inzulin lidský krev MeSH
- inzulinová rezistence * MeSH
- ketolátky * analýza MeSH
- klinické laboratorní techniky metody statistika a číselné údaje MeSH
- lidé MeSH
- omezení příjmu potravy krev MeSH
- triglyceridy krev MeSH
- Check Tag
- lidé MeSH