A 2-year longitudinal study of enzyme-linked immunosorbent assay (ELISA) antibodies against Phlebotomus perniciosus and Phlebotomus papatasi (Diptera: Psychodidae) sandfly saliva was performed in 32 Beagle dogs treated preventively with an imidacloprid-permethrin topical insecticide in an endemic area in Spain. Dogs were grouped into three sandfly exposure groups according to the time of inclusion in the study. Assays analysed immunoglobulin G (IgG) against salivary gland homogenates (SGH) of both species and recombinant P. papatasi rSP32 and P. perniciosus rSP03B proteins in serum. The dogs were participating in a Leishmania infantum (Kinetoplastida: Trypanosomatidae) vaccine trial and were experimentally infected with the parasite in the second year. No dog acquired natural L. infantum infections during the first year, but most developed anti-saliva antibodies, and median log-transformed optical densities (LODs) were seasonal, mimicking those of local sandflies. This indicates that the repellent efficacy of the insecticide used is below 100%. Multi-level modelling of LODs revealed variability among dogs, autocorrelation and differences according to the salivary antigen and the dog's age. However, dog seroprevalence, estimated using pre-exposure LODs as cut-offs, was relatively low. This, and the fact that dogs did not become naturally infected with L. infantum, would support the efficacy and usefulness of this imidacloprid-permethrin topical insecticide in canine leishmaniasis control.
- MeSH
- biologické markery krev MeSH
- dusíkaté sloučeniny aplikace a dávkování farmakologie MeSH
- kousnutí a bodnutí hmyzem prevence a kontrola MeSH
- longitudinální studie MeSH
- neonikotinoidy aplikace a dávkování farmakologie MeSH
- permethrin aplikace a dávkování farmakologie MeSH
- Phlebotomus účinky léků MeSH
- protilátky krev účinky léků MeSH
- psi imunologie MeSH
- repelenty proti hmyzu aplikace a dávkování farmakologie MeSH
- zvířata MeSH
- Check Tag
- psi imunologie MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Španělsko MeSH
- Klíčová slova
- Bio Anti Mosquito sprej,
- MeSH
- Culex parazitologie patogenita účinky léků MeSH
- kousnutí a bodnutí hmyzem komplikace prevence a kontrola MeSH
- lidé MeSH
- oleje prchavé terapeutické užití MeSH
- piperidiny aplikace a dávkování farmakologie terapeutické užití MeSH
- repelenty proti hmyzu aplikace a dávkování klasifikace terapeutické užití MeSH
- Check Tag
- lidé MeSH
