Nanotechnology, new fascinating field of science, is bringing many application's options. However, it is necessary to understand their potential environmental risk and toxicity. Zinc selenide quantum dots (ZnSe QDs) are getting valuable due to wide industrial usage, mainly as cadmium free diodes or stabilizing ligand. Thanks to unique properties, they could also open the possibilities of application in the agriculture. Their effects on living organisms, including plants, are still unknown. Therefore, the attention of this work was given to antioxidant response of Arabidopsis thaliana to 100 and 250 μM ZnSe QDs foliar feeding. ZnSe QDs treatment had no statistically significant differences in morphology but led to increased antioxidant response in the leaves at the level of gene expression and production secondary antioxidant metabolites. Concurrently, analysis of growth properties of Agrobacterium tumefaciens was done. 250 μM ZnSe solution inhibited the Agrobacterium tumefaciens viability by 60%. This is the first mention about effect ZnSe QDs on the plants. Although QDs induced oxidative stress, the apply treatment dose of ZnSe QDs did not have significant toxic effect on the plants and even no morphological changes were observed. However, the same amount of ZnSe QD induced an inhibitory effect on Agrobacterium tumefaciens.
- MeSH
- Agrobacterium tumefaciens účinky léků růst a vývoj MeSH
- antioxidancia metabolismus MeSH
- Arabidopsis genetika růst a vývoj metabolismus MeSH
- exprese genu * MeSH
- kvantové tečky toxicita MeSH
- metabolomika * MeSH
- sloučeniny selenu aplikace a dávkování toxicita MeSH
- sloučeniny zinku aplikace a dávkování toxicita MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Acute toxicity of zinc oxide nanoparticle (ZnO-NP, mean particle size diameter of 10 nm) powder and water-soluble salt of zinc (ZnCl2) to annelid Enchytraeus crypticus was tested using an agar-based nutrient-enriched medium with the addition of kaolin and humic acids (HA). Adults of the E. crypticus were cultivated in pure agar and in three types of modified exposure media containing different proportions of model soil constituents. Potworms were exposed to zinc in both forms (1-1000 mg kg-1 of agar) for 96 h. In experiments with ZnCl2, toxicity of zinc was the highest in pure agar followed by agar with HA and agar with kaolin and HA and the lowest toxicity was observed in agar with kaolin. The corresponding LC50 values were 13.2, 28.8, 39.4, and 75.4 mg kg-1 respectively. In contrast, zinc in the form of ZnO-NPs was most toxic in the presence of HA followed by pure agar, agar with kaolin, and kaolin with HA. In this case, LC50 values were 15.8, 43.5, 111, and 122 mg kg-1 respectively. Scanning electron microscopy revealed that the smallest agglomerates occurred in the presence of kaolin, where ZnO-NPs were sealed in a kaolin shell. This effect reduced the bioavailability and toxicity of the NPs. In contrast, larger agglomerates were observed in the presence of HA but a larger amount of zinc was dispersed in the volume of agar.
- MeSH
- agar analýza MeSH
- chloridy toxicita MeSH
- kovové nanočástice toxicita MeSH
- kultivační média analýza MeSH
- látky znečišťující půdu toxicita MeSH
- Oligochaeta účinky léků růst a vývoj MeSH
- oxid zinečnatý toxicita MeSH
- sloučeniny zinku toxicita MeSH
- testy akutní toxicity metody MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Nanomaterials are currently the subject of intense research due to their wide variety of potential applications in the biomedical, optical and electronic fields. We prepared and tested cobalt zinc ferrite nanoparticles (Co0.5Zn0.5Fe2O4+γ [CZF-NPs]) encapsulated by amorphous silica in order to find a safe contrast agent and magnetic label for tracking transplanted cells within an organism using magnetic resonance imaging (MRI). Rat mesenchymal stem cells (rMSCs) were labeled for 48 h with a low, medium or high dose of CZF-NPs (0.05; 0.11 or 0.55 mM); silica NPs (Si-NPs; 0.11 mM) served as a positive control. The internalization of NPs into cells was verified by transmission electron microscopy. Biological effects were analyzed at the end of exposure and after an additional 72 h of cell growth without NPs. Compared to untreated cells, Annexin V/Propidium Iodide labeling revealed no significant cytotoxicity for any group of treated cells and only a high dose of CZF-NPs slowed down cell proliferation and induced DNA damage, manifested as a significant increase of DNA-strand breaks and oxidized DNA bases. This was accompanied by high concentrations of 15-F2t-isoprostane and carbonyl groups, demonstrating oxidative injury to lipids and proteins, respectively. No harmful effects were detected in cells exposed to the low dose of CZF-NPs. Nevertheless, the labeled cells still exhibited an adequate relaxation rate for MRI in repeated experiments and ICP-MS confirmed sufficient magnetic label concentrations inside the cells. The results suggest that the silica-coated CZF-NPs, when applied at a non-toxic dose, represent a promising contrast agent for cell labeling.
- MeSH
- barvení a značení MeSH
- buněčné kultury MeSH
- isoprostany metabolismus MeSH
- karbonylace proteinů účinky léků MeSH
- kobalt chemie toxicita MeSH
- kontrastní látky chemie toxicita MeSH
- krysa rodu rattus MeSH
- kultivované buňky MeSH
- magnetická rezonanční tomografie MeSH
- metabolismus lipidů účinky léků MeSH
- mezenchymální kmenové buňky účinky léků metabolismus ultrastruktura MeSH
- nanočástice chemie toxicita MeSH
- oxid křemičitý chemie toxicita MeSH
- poškození DNA * MeSH
- povrchové vlastnosti MeSH
- proliferace buněk účinky léků MeSH
- sloučeniny zinku chemie toxicita MeSH
- transmisní elektronová mikroskopie MeSH
- viabilita buněk účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- železité sloučeniny chemie toxicita MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
