Use of the crustacean Artemia franciscana for alternative biotests of the second generation was studied, and possible experimental design and applications of such tests outlined. In addition to the classical use in ecotoxicology, the test can be used in pharmacology as well, or to monitor the effects of ionizing radiation in co-exposure with some chemical compounds. The synergistic effect of co-exposure of PCB (DELOR 103), cadmium chloride and potassium dichromate with beta 89Sr irradiation was shown. We also demonstrated the anti-oxidative and pro-oxidative effects of the ascorbic acid in dependence on its concentration. Use of the pharmaco-toxicological screening in search for the novel inhibitors of cyclin-dependent kinases was demonstrated as well, showing that Artemia franciscana may be used as a suitable biosensor instead of the expensive tests on higher vertebrates.
- Klíčová slova
- DELOR 103, letalita, synergický efekt, biotesty,
- MeSH
- Artemia * MeSH
- chlorid kademnatý toxicita MeSH
- dvojchroman draselný toxicita MeSH
- inhibiční proteiny cyklin-dependentních kinas toxicita MeSH
- ionizující záření MeSH
- kyselina askorbová aplikace a dávkování farmakologie MeSH
- peroxid vodíku MeSH
- radioizotopy stroncia toxicita MeSH
- testy toxicity * MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
The aim of the study was to find the sources of 137Cs in wild boar food in the natural ecosystem. The main emphasis is focused on the analyses of wild boar muscles and the content of wild boar stomach. Boars weighing 20 to 100 kg were killed at two locations. The highest specific activities of muscles were measured in boars originated from the Dvorce location; the average specific activity in boars killed on 14 April 2007 and 9 March 2008 achieved 132 Bq kg-1. Due to high fluctuation the differences between the mean values of 31 Bq kg-1 in males and 43 Bq kg-1 in females were not significant. Earthworms from grass fields with a specific activity of 16 Bq kg-1, rootlets from the Šabrava location with 200 Bq kg-1 and Elaphomyces granulatus fruiting bodies with 4,743 Bq kg-1 and 2,858 Bq kg-1 are the components of boar food with the 137Cs specific activities higher than that of the detection limit. Consequently, underground mushrooms probably represent the main source of radiocesium in the food chain of boars. A remarkable reduction of 137Cs specific activities in boar muscles is not expected at the post-Chernobyl radiocesium contaminated locations with the occurrence of Elaphomyces granulatus within next two decades.
- Klíčová slova
- Elaphomyces granulatus, migrace radionuklidů,
- MeSH
- Ascomycota MeSH
- divoká zvířata MeSH
- maso MeSH
- prasata MeSH
- radioaktivní kontaminace potravin * MeSH
- radioaktivní znečišťující látky analýza MeSH
- radioizotopy cesia * MeSH
- Sus scrofa MeSH
- únik radioaktivních látek MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
