• MeSH
• Drug Resistance, Bacterial genetics   immunology   MeSH
• Enterococcus faecalis genetics   drug effects   MeSH
• Enterococcus faecium genetics   drug effects   MeSH
• Vancomycin-Resistant Enterococci * genetics   pathogenicity   drug effects   MeSH
• Phenotype MeSH
• Genotype MeSH
• Glycopeptides pharmacology   metabolism   therapeutic use   MeSH
• Cross Infection microbiology   prevention & control   MeSH
• Humans MeSH
• Check Tag
• Humans MeSH
• Publication type
• Research Support, Non-U.S. Gov't MeSH
• Review MeSH

The study aimed to determine the occurrence of Enterobacteriaceae producing broad-spectrum beta-lactamases, vancomycin-resistant enterococci (VRE) and methicillin-resistant Staphylococcus aureus strains in poultry in Moravia, Czech Republic, including phenotypic and genotypic analyses of the extent of resistance. Using chromogenic screening media, a total of 240 clinical samples collected from poultry and the poultry farm environment were processed. Phenotypic tests identified 23 isolates of broad-spectrum beta-lactamase-producing Escherichia coli and one VRE isolate (Enterococcus faecium with VanA resistance). Methicillin-resistant Staphylococcus aureus strains were not detected. Among the isolates producing broad-spectrum beta-lactamases, 17 produced extended-spectrum beta-lactamases, most frequently CTX-M; the remaining 6 isolates were CIT-type AmpC enzymes. No carbapenemase-producing strains were detected. Pulsed-field gel electrophoresis showed that 21 E. coli strains (91%) were genetically unrelated isolates. Increasing resistance of bacteria to antibiotic agents poses a serious issue for both human and veterinary medicine globally. For humans, a potential source of resistant bacteria may be animals or their products entering the human food chain, for example poultry. The presented study extends existing knowledge about the occurrence of resistant bacteria in poultry in Moravia and describes the phenotype and genotype of their resistance to antibiotics.

• MeSH
• Drug Resistance, Bacterial * MeSH
• beta-Lactamases * analysis   adverse effects   MeSH
• Poultry * microbiology   MeSH
• Poultry Products * microbiology   adverse effects   MeSH
• Vancomycin-Resistant Enterococci pathogenicity   MeSH
• Escherichia coli * genetics   isolation & purification   pathogenicity   MeSH
• Food Contamination MeSH
• Methicillin-Resistant Staphylococcus aureus MeSH
• Microbiological Techniques MeSH
• Research MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Geographicals
• Czech Republic MeSH