In an effort to delineate how cholesterol protects membrane structure under oxidative stress conditions, we monitored the changes to the structure of lipid bilayers comprising 30 mol% cholesterol and an increasing concentration of Class B oxidized 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) glycerophospholipids, namely, 1-palmitoyl-2-(9'-oxo-nonanoyl)-sn-glycero-3-phosphocholine (PoxnoPC), and 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PazePC), using atomistic molecular dynamics simulations. Increasing the content of oxidized phospholipids (oxPLs) from 0 to 60 mol% oxPL resulted in a characteristic reduction in bilayer thickness and increase in area per lipid, thereby increasing the exposure of the membrane hydrophobic region to water. However, cholesterol was observed to help reduce water injury by moving into the bilayer core and forming more hydrogen bonds with the oxPLs. Cholesterol also resists altering its tilt angle, helping to maintain membrane integrity. Water that enters the 1-nm-thick core region remains part of the bulk water on either side of the bilayer, with relatively few water molecules able to traverse through the bilayer. In cholesterol-rich membranes, the bilayer does not form pores at concentrations of 60 mol% oxPL as was shown in previous simulations in the absence of cholesterol.

• MeSH
• cholesterol chemie   metabolismus   MeSH
• fosfatidylcholiny chemie   metabolismus   MeSH
• fosfolipidy chemie   metabolismus   MeSH
• fosforylcholin analogy a deriváty   chemie   metabolismus   MeSH
• lipidové dvojvrstvy chemie   metabolismus   MeSH
• oxidace-redukce MeSH
• oxidační stres MeSH
• simulace molekulární dynamiky MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• MeSH
• Alzheimerova nemoc patologie   MeSH
• amyloid MeSH
• diferenciální diagnóza MeSH
• genetické nemoci vrozené MeSH
• lidé středního věku MeSH
• nemoci centrálního nervového systému diagnóza   patologie   MeSH
• Check Tag
• lidé středního věku MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH