A highly prospective drug for the X-ray induced photodynamic therapy (PDTX), LuAG:Pr3+@SiO2-PpIX nanocomposite, was successfully prepared by a three step process: photo-induced precipitation of the Lu3Al5O12:Pr3+(LuAG:Pr3+) core, sol-gel technique for amorphous silica coating, and a biofunctionalization by attaching the protoporphyrin IX (PpIX) molecules. The synthesis procedure provides three-layer nanocomposite with uniform shells covering an intensely luminescent core. Room temperature radioluminescence (RT RL) spectra as well as photoluminescence (RT PL) steady-state and time resolved spectra of the material confirm the non-radiative energy transfer from the core Pr3+ions to the PpIX outer layer. First, excitation of Pr3+ions results in the red luminescence of PpIX. Second, the decay measurements exhibit clear evidence of mentioned non-radiative energy transfer (ET). The singlet oxygen generation in the system was demonstrated by the 3'-(p-aminophenyl) fluorescein (APF) chemical probe sensitive to the singlet oxygen presence. The RT PL spectra of an X-ray irradiated material with the APF probe manifest the formation of singlet oxygen due to which enhanced luminescence around 530 nm is observed. Quenching studies, using NaN3as an1O2inhibitor, also confirm the presence of1O2in the system and rule out the parasitic reaction with OH radicals. To summarize, presented features of LuAG:Pr3+@SiO2-PpIX nanocomposite indicate its considerable potential for PDTX application.
This study describes a one-pot synthesis of superparamagnetic maghemite-based 4-aminobenzoic acid-coated spherical core-shell nanoparticles (PABA@FeNPs) as suitable nanocomposites potentially usable as magnetic carriers for drug delivery. The PABA@FeNPs system was subsequently functionalized by the activated species (1* and 2*) of highly in vitro cytotoxic cis-[PtCl2(3Claza)2] (1; 3Claza stands for 3-chloro-7-azaindole) or cis-[PtCl2(5Braza)2] (2; 5Braza stands for 5-bromo-7-azaindole), which were prepared by a silver(I) ion assisted dechlorination of the parent dichlorido complexes. The products 1*@PABA@FeNPs and 2*@PABA@FeNPs, as well as an intermediate PABA@FeNPs, were characterized by a combination of various techniques, such as Mössbauer, FTIR and EDS spectroscopy, thermal analysis, SEM and TEM. The results showed that the products consist of well-dispersed maghemite-based nanoparticles of 13 nm average size that represent an easily obtainable system for delivery of highly cytotoxic cisplatin-like complexes in oncological practice.
- MeSH
- Cisplatin administration & dosage chemistry MeSH
- Indoles chemical synthesis chemistry MeSH
- 4-Aminobenzoic Acid administration & dosage chemical synthesis chemistry MeSH
- Drug Delivery Systems * MeSH
- Humans MeSH
- Neoplasms drug therapy MeSH
- Nanoparticles chemistry MeSH
- Drug Carriers chemical synthesis chemistry MeSH
- Antineoplastic Agents chemistry MeSH
- Silver chemistry MeSH
- Ferric Compounds chemical synthesis chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
This paper reports on the synthesis and characterization of a novel electrochemical label for sensitive electrochemical stripping metalloimmunoassays based on silver dendrimer-encapsulated nanoparticles (NPs). Silver dendrimer nanocomposites (Ag-DNCs) were synthesized from a generation 5-7 (G5-7) hydroxyl-terminated ethylenediamine-core-type (2-carbon core) PAMAM dendrimer. Several fixed ratios of Ag(+)/dendrimer were prepared with the aim to obtain stable nanocomposites with maximal silver loading in the interior of a polymeric shell. Synthesized Ag-DNCs were characterized by UV-vis spectrophotometry, atomic force microscopy (AFM), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). The G7 Ag-DNC synthesized in 2000 molar excess of silver (1/4 ratio of tertiary amine/Ag(+)) turned out a more suitable candidate for the label development. By combination of the differential pulse voltammetry (DPV) and the anodic stripping analysis (ASV) on a carbon electrode, down to 1.35 x 10(+10) of individual Ag-DNCs (LOD=0.9 pM, 25 ml volume) was detected after the dissolution of silver nanoparticles in a diluted nitric acid. The potential advantages of proposed electrochemical label are discussed.
- MeSH
- Equipment Failure Analysis MeSH
- Staining and Labeling methods MeSH
- Coated Materials, Biocompatible chemistry MeSH
- Biosensing Techniques instrumentation MeSH
- Dendrimers chemistry MeSH
- Equipment Design MeSH
- Electrochemistry instrumentation MeSH
- Financing, Organized MeSH
- Immunoassay instrumentation MeSH
- Nanoparticles chemistry ultrastructure MeSH
- Nanotechnology methods instrumentation MeSH
- Reproducibility of Results MeSH
- Sensitivity and Specificity MeSH
- Silver chemistry MeSH
