Ibbotson, A* Dotaz Zobrazit nápovědu
To validate age determination from scales in European grayling Thymallus thymallus, the scale-read age of fish was compared with the true age obtained by tag-recapture analysis. A total of 3997 individuals were tagged with visible implant tags and passive integrated transponder (PIT) tags in the River Wylye, south-west England during 1999-2007. Annual repeat surveys were undertaken and collected scales read without prior knowledge of tag-recapture age. Accuracy of fish ageing by scales was highest in 1 and 2 year-old fish but decreased in older fish. In later life stages (>4 years old), underestimation of age occurred and the error in reading scales rose to 51.9% in 5 year-old fish. Age assigned from scales underestimated the tag-recapture assigned age by as much as 3 years. This study suggests that use of scales is an appropriate method to age a short-lived population of T. thymallus inhabiting productive lotic systems. The underestimation of age in older fish, however, needs to be considered in the management of fish stocks because it may lead to undesirable exploitation of population.
- MeSH
- Salmonidae růst a vývoj fyziologie MeSH
- stárnutí MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- validační studie MeSH
- Geografické názvy
- Anglie MeSH
- Klíčová slova
- fototesty, fotosenzitivita,
- MeSH
- dospělí MeSH
- kohortové studie * MeSH
- lidé středního věku MeSH
- lidé MeSH
- prognóza MeSH
- progrese nemoci MeSH
- rizikové faktory MeSH
- světlo MeSH
- urtikarie * diagnóza epidemiologie terapie MeSH
- věkové faktory MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Geografické názvy
- Spojené království MeSH
The activity of the tumor suppressor protein p53 is controlled by a balance between E3-ligase mediated p53 protein degradation and protein kinase-mediated assembly of p53:p300 transcription machinery. Genetic studies in mice have shown that mutation of the CK2 phospho-acceptor site in p53 increases UV-induced skin cancer formation,(11) highlighting an unexpected role for p53 phosphorylation in mediating p53-dependent tumor suppression. However, it is not known in which cell types CK2-mediated phosphorylation of p53 occurs. Using human skin as a model to determine whether there is cell-selectivity in modulating p53 phosphorylation, we have found a selective induction of p53 phosphorylation at the CK2-site in the basal cells of UV irradiated human skin. Dual-immunofluorescence also revealed that Ser392 and Ser15 phosphorylation of p53 also occur in the same basal cells, although often within distinct regions of the nucleus. Given that p63alphaDeltaN is required for p53 activation after DNA damage, we examined and found a high proportion of cells co-express p63alphaDeltaN and CK2-phosphorylated p53 after UV-irradiation. As controls, the proliferation marker Ki67 and p63alphaDeltaN generally exhibit mutually exclusive expression. These data identify a physiological model with which to identify signaling pathways that mediate cross-talk between p63alphaDeltaN and activating p53 kinase pathways after DNA damage in basal cell populations.
- MeSH
- DNA vazebné proteiny metabolismus MeSH
- fosforylace MeSH
- kaseinkinasa II * fyziologie MeSH
- kmenové buňky cytologie metabolismus MeSH
- kůže * metabolismus účinky záření MeSH
- lidé MeSH
- myši MeSH
- nádorové supresorové proteiny metabolismus MeSH
- nádorový supresorový protein p53 chemie metabolismus MeSH
- poškození DNA MeSH
- progrese nemoci MeSH
- regulace genové exprese * MeSH
- signální transdukce MeSH
- trans-aktivátory metabolismus MeSH
- transkripční faktory MeSH
- ubikvitinligasy metabolismus MeSH
- ultrafialové záření MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
- MeSH
- finanční podpora výzkumu jako téma MeSH
- fluorouracil aplikace a dávkování terapeutické užití MeSH
- fotochemoterapie metody trendy využití MeSH
- interpretace statistických dat MeSH
- kryoterapie metody trendy využití MeSH
- kyseliny levulové terapeutické užití MeSH
- lidé MeSH
- spinocelulární karcinom farmakoterapie terapie MeSH
- výsledek terapie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- multicentrická studie MeSH
- randomizované kontrolované studie MeSH
- MeSH
- fototerapie metody MeSH
- lidé MeSH
- psoriáza terapie MeSH
- terapie ultrafialovými paprsky metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- randomizované kontrolované studie MeSH
- srovnávací studie MeSH
- MeSH
- adrenální insuficience farmakoterapie krev MeSH
- cirkadiánní rytmus MeSH
- dospělí MeSH
- hormonální substituční terapie metody MeSH
- hydrokortison aplikace a dávkování farmakokinetika MeSH
- klinické zkoušky jako téma MeSH
- klinické zkoušky kontrolované jako téma MeSH
- lidé MeSH
- plocha pod křivkou MeSH
- přijímání potravy MeSH
- prospektivní studie MeSH
- rozvrh dávkování léků MeSH
- senioři MeSH
- tělesná hmotnost MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- souhrny MeSH
The TP53 mutation profile in chronic lymphocytic leukemia (CLL) and the correlation of TP53 mutations with allele status or associated molecular genetics are currently unknown. We performed a large mutation analysis of TP53 at four centers and characterized the pattern of TP53 mutations in CLL. We report on 268 mutations in 254 patients with CLL. Missense mutations appeared in 74% of cases compared with deletions and insertions (20%), nonsense (4%) and splice site (2%) mutations. The majority (243 of 268) of mutations were located in the DNA-binding domain. Transitions were found in 131 of 268 mutations, with only 41 occurring at methylated CpG sites (15%), suggesting that transitions at CpGs are uncommon. The codons most frequently mutated were at positions 175, 179, 248 and 273; in addition, we detected a common 2-nt deletion in the codon 209. Most mutations (199 of 259) were accompanied by deletion of the other allele (17p-). Interestingly, trisomy 12 (without 17p-) was only found in one of 60 cases with TP53 mutation (without 17p-) compared with 60 of 16 in the cohort without mutation (P=0.006). The mutational profile was not different in the cohorts with and without previous therapy, suggesting that the mechanism underlying the development of mutations may be similar, independent of treatment.
- MeSH
- chronická lymfatická leukemie * genetika MeSH
- CpG ostrůvky MeSH
- geny p53 * MeSH
- lidé MeSH
- mutace * MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Gamboni -- 10 Alopecia Areata 29 -- Sivanie Vivehanantha, John Berth-Jones -- 11 Amyloidosis 34 -- William Thomas A. Ibbotson, Robed S. Allen, Vanessa Venning -- Mycetoma: Eumycetoma and Actinomycetoma . -- Mahreen Ameen, Wanda Sonia Robles Graham A.
4th ed. xxvi, 861 s. : il., tab. ; 28 cm
- MeSH
- kožní nemoci klasifikace terapie MeSH
- management nemoci MeSH
- nemoci kůže a pojivové tkáně terapie MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- dermatovenerologie
- NLK Publikační typ
- kolektivní monografie