The aim of this study was to compare the expression of selected genes in bovine embryos developed from oocytes with different developmental competence. Four oocyte populations were collected, separately either from small (2-5 mm) or medium (6-10 mm) follicles, in the growth/stagnation (G/S) or dominance/regression (D/R) stage of the first follicular wave. They were matured, fertilized and cultured to D7 or D8 blastocysts by a standard protocol. Poly (A)+ mRNA was extracted from pooled blastocysts and the expression of bax-alpha (Bax), connexin 43 (Cx 43) and connexin 31 (Cx 31) was estimated using real-time RT-PCR. The cleavage rates were significantly higher in oocytes collected from both medium and small follicles, (p < or = 0.05 and p < or = 0.01, respectively) in the G/S than in the D/R stage. There were no significant differences in the D7 blastocyst rates between oocytes from both medium and small follicles in the G/S or D/R stage. But the D8 blastocyst rate was significantly higher in oocytes from small follicles in the G/S stage compared with those in the D/R stage. The relative abundance of Bax and Cx 31 made no significant difference in both D7 and D8 blastocysts developed from oocytes collected from medium or small follicles in the G/S or D/R stages. But the relative abundance of the Cx 43 transcript was significantly higher in D8 blastocysts developed from oocytes collected from both medium and small follicles in the G/S stage compared with those in the D/R stage. We conclude that the relative abundance of Cx 43 can be used as a marker of developmental potential for embryos derived from oocytes with different developmental competence because the level of Cx 43 transcript was greater in embryos derived from oocytes with greater developmental competence compared with those derived from oocytes with lesser developmental competence.

• MeSH
• blastocysta fyziologie   MeSH
• embryo savčí metabolismus   MeSH
• embryonální vývoj MeSH
• exprese genu MeSH
• financování organizované MeSH
• konexin 43 genetika   MeSH
• konexiny genetika   MeSH
• messenger RNA analýza   izolace a purifikace   MeSH
• oocyty fyziologie   MeSH
• ovariální folikul cytologie   růst a vývoj   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• protein X asociovaný s bcl-2 genetika   MeSH
• skot embryologie   MeSH
• zvířata MeSH
• Check Tag
• skot embryologie   MeSH
• ženské pohlaví MeSH
• zvířata MeSH

AIMS: To determine susceptibility of Clostridium perfringens strains CCM 4435(T) and CNCTC 5459 to C(2)-C(18) fatty acids, and evaluate influence of pH in cultures grown on glucose. Straw particles were added to cultures to simulate the presence of solid phase of the digestive tract milieu. METHODS AND RESULTS: Antimicrobial activity of fatty acids was expressed as a concentration at which only 50% of the initial glucose was utilized. Lauric acid showed the highest antimicrobial activity, followed by myristic, capric, oleic and caprylic acid. Only strain CNCTC 5459 was susceptible to linoleic acid. Neither caproic acid and acids with a shorter carbon chain nor palmitic and stearic acid influenced substrate utilization. The antimicrobial activity of myristic, oleic and linoleic acid decreased when clostridia were grown in the presence of straw particles. In cultures of both strains treated with capric and lauric acid at pH 5.0-5.3, the number of viable cells was <10(2) ml(-1). Only lauric acid reduced number of viable cells of both strains below 10(2) ml(-1) at pH > 6. Transmission electron microscopy revealed separation of inner and outer membranes and cytoplasma disorganization in cells treated with lauric acid. CONCLUSIONS: Lauric acid had the highest activity towards C. perfringens among fatty acid tested. Its activity was not influenced by the presence of solid particles and did not cease at pH > 6. SIGNIFICANCE AND IMPACT OF THE STUDY: Lauric acid might be a means for control of clostridial infections in farm animals.

• MeSH
• Clostridium perfringens růst a vývoj   účinky léků   ultrastruktura   MeSH
• financování organizované MeSH
• glukosa MeSH
• klostridiové infekce prevence a kontrola   veterinární   MeSH
• koncentrace vodíkových iontů MeSH
• kultivační média MeSH
• kyseliny laurové farmakologie   MeSH
• mastné kyseliny aplikace a dávkování   farmakologie   MeSH
• mikrobiální testy citlivosti MeSH
• potravinářské přísady aplikace a dávkování   MeSH
• transmisní elektronová mikroskopie MeSH

• MeSH
• estrální cyklus MeSH
• finanční podpora výzkumu jako téma MeSH
• folikulární fáze fyziologie   MeSH
• messenger RNA metabolismus   MeSH
• mezibuněčné signální peptidy a proteiny genetika   metabolismus   MeSH
• oocyty metabolismus   MeSH
• ovariální folikul cytologie   metabolismus   MeSH
• skot MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH
• Publikační typ
• srovnávací studie MeSH

• MeSH
• lidé MeSH
• nádory děložního čípku * mikrobiologie   MeSH
• onkogenní proteiny virové * imunologie   MeSH
• Papillomavirus E7 - proteiny MeSH
• protilátky virové * krev   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• dopisy MeSH