Infections caused by Staphylococcus aureus are a serious global threat, and with the emergence of antibiotic resistance, even more difficult to treat. One of the possible complications in antistaphylococcal therapy represents negative interactions of antibiotics with food. In this study, the in vitro interaction between oxacillin and crude palm seed oil from Astrocaryum vulgare, Cocos nucifera, and Elaeis guineensis against nine strains of S. aureus was determined using the checkerboard method. Lauric acid was identified as a major constituent of all tested oils by gas chromatography. The results showed strong concentration dependent antagonistic interactions between palm oils and oxacillin with values of fractional inhibitory concentrations indices ranging from 4.02 to 8.56 at concentrations equal or higher than 1024 µg/mL of the tested oils. Similarly, lauric acid in combination with oxacillin produced antagonistic action with fractional inhibitory concentration indices ranging from 4.01 to 4.28 at 1024 µg/mL. These findings suggest that interference between oxacillin and palm oils and their constituents can negatively affect the treatment of staphylococcal infections in humans and other animals.
- MeSH
- antagonismus léků MeSH
- antibakteriální látky farmakologie MeSH
- kyseliny laurové farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- oxacilin analogy a deriváty antagonisté a inhibitory farmakologie MeSH
- ropa analýza MeSH
- Staphylococcus aureus účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Thermogenic uncoupling has been proven only for UCP1 in brown adipose tissue. All other isoforms of UCPs are potentially acting in suppression of mitochondrial reactive oxygen species (ROS) production. In this contribution we show that BAT mitochondria can be uncoupled by lauric acid in the range of approximately 100 nM when endogenous fatty acids are combusted by carnitine cycle and beta-oxidation is properly separated from the uncoupling effect. Respiration increased up to 3 times when related to the lowest fatty acid content (BSA present plus carnitine cycle). We also illustrated that any effect leading to more coupled states leads to enhanced H2O2 generation and any effect resulting in uncoupling gives reduced H2O2 generation in BAT mitochondria. Finally, we report doubling of plant UCP transcript in cells as well as amount of protein detected by 3H-GTP-binding sites in mitochondria of shoots and roots of maize seedlings subjected to the salt stress.
- MeSH
- buněčné dýchání MeSH
- financování organizované MeSH
- guanosintrifosfát metabolismus MeSH
- hnědá tuková tkáň metabolismus MeSH
- iontové kanály MeSH
- karnitin metabolismus MeSH
- kořeny rostlin metabolismus MeSH
- kukuřice setá metabolismus MeSH
- kyseliny laurové farmakologie metabolismus MeSH
- mastné kyseliny metabolismus MeSH
- membránové proteiny fyziologie MeSH
- mitochondriální proteiny MeSH
- mitochondrie metabolismus MeSH
- oxidační stres MeSH
- peroxid vodíku metabolismus MeSH
- reaktivní formy kyslíku metabolismus MeSH
- rostlinné proteiny fyziologie MeSH
- rozpřahující látky farmakologie metabolismus MeSH
- savci MeSH
- techniky in vitro MeSH
- transportní proteiny fyziologie MeSH
- vazebná místa MeSH
- výhonky rostlin metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- přehledy MeSH
AIMS: To determine susceptibility of Clostridium perfringens strains CCM 4435(T) and CNCTC 5459 to C(2)-C(18) fatty acids, and evaluate influence of pH in cultures grown on glucose. Straw particles were added to cultures to simulate the presence of solid phase of the digestive tract milieu. METHODS AND RESULTS: Antimicrobial activity of fatty acids was expressed as a concentration at which only 50% of the initial glucose was utilized. Lauric acid showed the highest antimicrobial activity, followed by myristic, capric, oleic and caprylic acid. Only strain CNCTC 5459 was susceptible to linoleic acid. Neither caproic acid and acids with a shorter carbon chain nor palmitic and stearic acid influenced substrate utilization. The antimicrobial activity of myristic, oleic and linoleic acid decreased when clostridia were grown in the presence of straw particles. In cultures of both strains treated with capric and lauric acid at pH 5.0-5.3, the number of viable cells was <10(2) ml(-1). Only lauric acid reduced number of viable cells of both strains below 10(2) ml(-1) at pH > 6. Transmission electron microscopy revealed separation of inner and outer membranes and cytoplasma disorganization in cells treated with lauric acid. CONCLUSIONS: Lauric acid had the highest activity towards C. perfringens among fatty acid tested. Its activity was not influenced by the presence of solid particles and did not cease at pH > 6. SIGNIFICANCE AND IMPACT OF THE STUDY: Lauric acid might be a means for control of clostridial infections in farm animals.
- MeSH
- Clostridium perfringens růst a vývoj účinky léků ultrastruktura MeSH
- financování organizované MeSH
- glukosa MeSH
- klostridiové infekce prevence a kontrola veterinární MeSH
- koncentrace vodíkových iontů MeSH
- kultivační média MeSH
- kyseliny laurové farmakologie MeSH
- mastné kyseliny aplikace a dávkování farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- potravinářské přísady aplikace a dávkování MeSH
- transmisní elektronová mikroskopie MeSH
- MeSH
- chloridy metabolismus MeSH
- hnědá tuková tkáň metabolismus účinky léků MeSH
- iontový transport účinky léků MeSH
- křečci praví MeSH
- kyseliny laurové farmakologie metabolismus účinky záření MeSH
- kyseliny palmitové farmakologie MeSH
- membránové proteiny metabolismus účinky léků MeSH
- techniky in vitro MeSH
- transportní proteiny metabolismus účinky léků MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- zvířata MeSH
