Q95379266 Dotaz Zobrazit nápovědu
- Klíčová slova
- EBLN, exaptace, endogenous Borna-like N elements,
- MeSH
- Bornaviridae genetika MeSH
- genom lidský * genetika MeSH
- integrace viru MeSH
- komponenty genomu MeSH
- lidé MeSH
- mutace MeSH
- Retroviridae MeSH
- virové geny * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
The avian sarcoma and leukosis virus (ASLV) family of retroviruses contains five highly related envelope subgroups (A to E) thought to have evolved from a common viral ancestor in the chicken population. Three genetic loci in chickens determine the susceptibility or resistance of cells to infection by the subgroup A to E ASLVs. Some inbred lines of chickens display phenotypes that are somewhere in between either efficiently susceptible or resistant to infection by specific subgroups of ASLV. The tvb gene encodes the receptor for subgroups B, D, and E ASLVs. The wild-type Tvb(S1) receptor confers susceptibility to subgroups B, D, and E ASLVs. In this study, the genetic defect that accounts for the altered susceptibility of an inbred chicken line, line M, to infection by ASLV(B), ASLV(D), and ASLV(E) was identified. The tvb gene in line M, tvb(r2), encodes a mutant Tvb(S1) receptor protein with a substitution of a serine for a cysteine at position 125 (C125S). Here, we show that the C125S substitution in Tvb(S1) significantly reduces the susceptibility of line M cells to infection by ASLV(B) and ASLV(D) and virtually eliminates susceptibility to ASLV(E) infection both in cultured cells and in the incidence and growth of avian sarcoma virus-induced sarcomas in chickens. The C125S substitution significantly reduces the binding affinity of the Tvb(S1) receptor for the subgroup B, D, and E ASLV envelope glycoproteins. These are the first results that demonstrate a possible role of the cysteine-rich domain 3 in the function of the Tvb receptors.
- MeSH
- alely MeSH
- Alpharetrovirus klasifikace patogenita MeSH
- DNA primery MeSH
- druhová specificita MeSH
- financování organizované MeSH
- fúze membrán MeSH
- genetická predispozice k nemoci MeSH
- infekce onkogenními viry virologie MeSH
- kultivované buňky MeSH
- kuřecí embryo MeSH
- molekulární sekvence - údaje MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- průtoková cytometrie MeSH
- retrovirové infekce virologie MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční homologie aminokyselin MeSH
- substituce aminokyselin MeSH
- virové receptory fyziologie genetika chemie MeSH
- zvířata MeSH
- Check Tag
- kuřecí embryo MeSH
- zvířata MeSH
- MeSH
- endogenní retroviry genetika MeSH
- lidé MeSH
- posttranskripční úpravy RNA imunologie MeSH
- pseudogeny genetika MeSH
- RNA virová genetika MeSH
- stabilita RNA genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- genetická terapie MeSH
- prionové nemoci etiologie MeSH
- zárodečné buňky růst a vývoj MeSH
- Publikační typ
- kongresy MeSH
