Delayed ejaculation belongs to the group of sexual disorders in men. The causes of delayed ejaculation or anejaculation are not exactly known. It is assumed that it can be caused by psychogenic or organic influences or their combinations. One of the causes of delayed ejaculation may be elevated prolactin levels, which may be increased by psychosocial stress, pituitary disorders or also treatment with selective serotonin reuptake inhibitors in the treatment of depression. We tested a selected group of 50 men who were diagnosed with a depressive disorder and whose antidepressant treatment lasted for at least 24 weeks. These patients reported long-term delayed ejaculation or, in some cases, anejaculation as comorbidity. The results showed significant Spearman's correlation between elevated prolactin levels and intravaginal ejaculation latency values (R = 0.45), as well as between Beck's Depression-II inventory and intravaginal ejaculation latency and latency values (R = 0.48).

• MeSH
• Depressive Disorder * MeSH
• Ejaculation MeSH
• Humans MeSH
• Premature Ejaculation * etiology   MeSH
• Prolactin MeSH
• Selective Serotonin Reuptake Inhibitors pharmacology   therapeutic use   MeSH
• Sexual Dysfunction, Physiological * etiology   MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Publication type
• Journal Article MeSH

Potíže s ejakulací jsou zejména u urologických nemocných poměrně časté a nezřídka vedou ke zhoršení kvality sexuálního života i partnerského soužití. Přesto jsou často opomíjené. Bohužel ne vždy jsou ovlivnitelné. Muže je třeba náležitě poučit o možných negativních vlivech urologické medikace i operačních výkonů na ejakulaci. Následující text se zaměřuje na anejakulaci, retrográdní ejakulaci, opožděnou ejakulaci a bolestivou ejakulaci.

Difficulties with ejaculation are particularly common among urological patients and often lead to deterioration in quality of sexual life and partnership. Yet are often not disussed. Unfortunatelly, not always are influenceable. Patients should be properly informed about possible negative effects of urological medication or surgical procedures on ejaculation. The following text focuses on anejaculation, retrograde ejaculation, delayed ejaculation and painful ejaculation.

• MeSH
• Adrenergic alpha-Antagonists pharmacology   therapeutic use   MeSH
• Ejaculatory Dysfunction * surgery   drug therapy   MeSH
• Physical Stimulation methods   instrumentation   MeSH
• Humans MeSH
• Sperm Retrieval MeSH
• Psychotherapy methods   MeSH
• Check Tag
• Humans MeSH

• MeSH
• Adult MeSH
• Ejaculation MeSH
• Fertilization MeSH
• Clinical Laboratory Techniques MeSH
• Humans MeSH
• Sperm Motility MeSH
• Infertility, Male diagnosis   MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Male MeSH

Mammalian fertilization remains a poorly understood event with the vast majority of studies done in the mouse model. The purpose of this review is to revise the current knowledge about semen deposition, sperm transport, sperm capacitation, gamete interactions and early embryonic development with a focus on the porcine model as a relevant, alternative model organism to humans. The review provides a thorough overview of post-ejaculation events inside the sow's reproductive tract including comparisons with humans and implications for human fertilization and assisted reproductive therapy (ART). Porcine methodology for sperm handling, preservation, in vitro capacitation, oocyte in vitro maturation, in vitro fertilization and intra-cytoplasmic sperm injection that are routinely used in pig research laboratories can be successfully translated into ART to treat human infertility. Last, but not least, new knowledge about mitochondrial inheritance in the pig can provide an insight into human mitochondrial diseases and new knowledge on polyspermy defense mechanisms could contribute to the development of new male contraceptives.

• MeSH
• Fertility physiology   MeSH
• Fertilization physiology   MeSH
• Sperm Capacitation physiology   MeSH
• Humans MeSH
• Disease Models, Animal MeSH
• Swine MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Review MeSH

Seminal plasma proteins bind the sperm surface at ejaculation and may modulate several aspects of sperm activity during reproduction. DQH sperm surface protein, present in boar seminal plasma, shows affinity to phoshorylcholine, acidic polysaccharides, oviductal epithelium and zona pellucida glycoproteins. Monoclonal antibodies (MAbs) against DQH protein were prepared and used for determination of the DQH protein origin in boar reproductive organs, its localization on boar spermatozoa, and for investigation of its binding abilities in the porcine oviduct and to the zona pellucida of the oocyte. The mRNA transcript of DQH protein was found in seminal vesicles and not in the testis, epididymis and prostate. Its translated products were immunodetected by MAbs in seminal vesicle extract and fluid, in seminal vesicle tissue sections and on the membrane-associated acrosomal part of ejaculated spermatozoa. These results confirm the ability of DQH protein to bind the sperm surface at ejaculation and to participate in formation of the sperm reservoir in the porcine oviduct. Moreover, monoclonal antibodies reduced binding of sperm to oocytes and proved the role of DQH protein in the sperm-zona pellucida primary binding.

• MeSH
• Fertilization MeSH
• Financing, Organized MeSH
• Immunohistochemistry MeSH
• Sperm-Ovum Interactions MeSH
• Humans MeSH
• Membrane Glycoproteins genetics   immunology   metabolism   MeSH
• Antibodies, Monoclonal MeSH
• Genitalia, Male metabolism   MeSH
• Reverse Transcriptase Polymerase Chain Reaction MeSH
• Swine MeSH
• Seminal Plasma Proteins metabolism   MeSH
• Semen MeSH
• Spermatozoa metabolism   MeSH
• Protein Binding MeSH
• Fallopian Tubes metabolism   MeSH
• Zona Pellucida metabolism   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Female MeSH
• Animals MeSH

• Keywords
• Psychiatrie, psychoterapie,
• MeSH
• Child Psychiatry MeSH
• Mental Disorders MeSH
• Psychiatric Nursing MeSH
• Psychiatric Rehabilitation MeSH
• Psychiatry MeSH
• Hospitals, Psychiatric MeSH

• NML Fields
• psychiatrie
• pediatrie

• MeSH
• Child Psychiatry MeSH
• Mental Disorders MeSH
• Psychiatric Nursing MeSH
• Psychiatric Rehabilitation MeSH
• Psychiatry MeSH
• Hospitals, Psychiatric MeSH
• Geographicals
• Czech Republic MeSH

• Conspectus
• Psychiatrie
• Učební osnovy. Vyučovací předměty. Učebnice
• NML Fields
• psychiatrie
• pediatrie
• NML Publication type
• učebnice vysokých škol

Male infertility is a serious problem in an increasing number of couples. We report an infertile man with non-obstructive azoospermia and karyotype 45,XY,rob(14;22). The immunofluorescence analysis of his testicular tissue using antibodies to SYCP1, SYCP3, HORMAD2, MLH1, and centromeres showed delayed synapsis of the chromosomes involved in the translocation, a varying extent of trivalent asynapsis and its association with sex chromosomes. The mean frequency of meiotic recombination per cell was within the range of normal values. Fluorescence in situ hybridization (FISH) with probes for chromosomes 14 and 22 revealed 5.83% of chromosomally abnormal testicular spermatozoa. FISH with probes for chromosomes X, Y, and 21 showed frequencies of disomic and diploid testicular spermatozoa increased when compared to ejaculated sperm of healthy donors, but comparable with published results for azoospermic patients. PGD by FISH for the translocation and aneuploidy of chromosomes X, Y, 13, 18, and 21 showed a normal chromosomal complement in one out of three analyzed embryos. A healthy carrier girl was born after the embryo transfer. This study shows the benefits of preimplantation genetic diagnosis in a case of a rare Robertsonian translocation carrier with azoospermia and a relatively low frequency of chromosomally unbalanced testicular spermatozoa.

• MeSH
• Aneuploidy * MeSH
• Azoospermia genetics   MeSH
• Genetic Carrier Screening * MeSH
• Karyotyping MeSH
• Humans MeSH
• Chromosomes, Human, Pair 14 * MeSH
• Chromosomes, Human, Pair 22 * MeSH
• Meiosis genetics   MeSH
• Spermatozoa metabolism   MeSH
• Translocation, Genetic * MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Publication type
• Journal Article MeSH
• Case Reports MeSH
• Research Support, Non-U.S. Gov't MeSH

• MeSH
• Physiology MeSH

• Conspectus
• Fyziologie člověka a srovnávací fyziologie
• Učební osnovy. Vyučovací předměty. Učebnice
• NML Fields
• fyziologie
• NML Publication type
• učebnice vysokých škol

Male germ cells experience a drastic chromatin remodeling through the nucleo-histone to nucleo-protamine (NH-NP) transition necessary for proper sperm functionality. Post-translational modifications (PTMs) of H4 Lys5, such as acetylation (H4K5ac), play a crucial role in epigenetic control of nucleosome disassembly facilitating protamine incorporation into paternal DNA. It has been shown that butyrylation on the same residue (H4K5bu) participates in temporal regulation of NH-NP transition in mice, delaying the bromodomain testis specific protein (BRDT)-dependent nucleosome disassembly and potentially marking retained nucleosomes. However, no information was available so far on this modification in human sperm. Here, we report a dual behavior of H4K5bu and H4K5ac in human normal spermatogenesis, suggesting a specific role of H4K5bu during spermatid elongation, coexisting with H4K5ac although with different starting points. This pattern is stable under different testicular pathologies, suggesting a highly conserved function of these modifications. Despite a drastic decrease of both PTMs in condensed spermatids, they are retained in ejaculated sperm, with 30% of non-colocalizing nucleosome clusters, which could reflect differential paternal genome retention. Whereas no apparent effect of these PTMs was observed associated with sperm quality, their presence in mature sperm could entail a potential role in the zygote.

• MeSH
• Acetylation MeSH
• Chromatin * metabolism   MeSH
• Histones metabolism   MeSH
• Humans MeSH
• Mice MeSH
• Nucleosomes * metabolism   MeSH
• Protein Processing, Post-Translational MeSH
• Protamines metabolism   MeSH
• Chromatin Assembly and Disassembly MeSH
• Semen metabolism   MeSH
• Spermatids metabolism   MeSH
• Spermatogenesis physiology   MeSH
• Spermatozoa metabolism   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH