The aim of this work was to experimentally evaluate the effect of feeding industrial emissions from a copper-producing plant upon Cu accumulation in the testes of six Merino breeding rams. In the experimental animals (4) the daily copper intake from the exhalations was 15 mg/kg l.w. Administration was continued until the haemolytic crisis appeared (on day 42 in one animal and on day 50 of the experiment in the other animals). The tissue of the testes was examined by both optic and electron microscopy. The patho-anatomical picture of the organs was characterized by general icterus which was also evident on the sectional surfaces of the testes. In the right and left testis of the experimental animals Cu levels were found that were twice higher than those of the controls (Tab. IV). The toxic effect of copper became evident by the destruction of all developmental stages of germinative cells mainly by that the integrity of the cell membranes was damaged thus resulting in a gradual release of the germinative cells, into the tubular lumen. In extreme cases the tubules were lined with nuclei only and the residual cytoplasm of Sertoli cells (Sertoli cells--only syndrome). Ruptures of the cytoplasmic membranes were also observed on the Leydig cells. Thickening of the tubular lamina propria was caused by homogenization and enormous invagination of the lamellar layer, which was rather predominant on the periphery of the organ (Fig. 2). In the deeper layers of the testes reduction of the cellular and non-cellular layers of the tubular walls occurred which frequently resulted in the rupture of the basement membrane.(ABSTRACT TRUNCATED AT 250 WORDS)

• MeSH
• látky znečišťující životní prostředí toxicita   MeSH
• měď toxicita   MeSH
• ovce anatomie a histologie   MeSH
• testis účinky léků   patologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• látky znečišťující životní prostředí MeSH
• měď MeSH

This work evaluated the effect of interactions between copper (Cu) and selenium (Se) in the course of experimentally-produced Cu intoxication with industrial emissions on the animals' life spans and the Cu and Se concentrations in serum, liver and kidneys. We selected 15 Valaska ewes 1.5 y old. After the morning feeding all the animals were given emissions from a nearby Cu plant that contained 16.5% Cu and 0.0093% Se. Seven of the animals (group B) were given 5 ml SELEVIT injectable (Biotika, Slovenska Lupca)/head before starting the experiment and again on days 30 and 60. In the animals without Se supplementation (group A) the daily Cu and Se intakes from the emission and food were 466.0 mg and 2.56 mg, respectively. The average life span of the sheep in group A was 77 d and in group B it was 79.83 d. The first death due to intoxication occurred on the day 65 in group A and on day 69 in group B. During the emission feeding period no statistically significant differences were observed in cupraemia between the ewes with Se supplementation and those without it. Hypercupraemia was found in both groups from day 50 till death. Only at the end of the experiment was the serum Cu of group A statistically lower than that in group B. Cu concentrations in the liver and kidneys (dry matter) of the sheep that died with Cu intoxication in group A was 2138 +/- 1090.96 mg/kg and 272.94 +/- 197.16 mg/kg, and in group B they were 2603 +/- 1332.9 mg/kg and 341.65 +/- 307.45 mg/kg, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

• MeSH
• játra metabolismus   MeSH
• látky znečišťující vzduch v pracovním prostředí metabolismus   otrava   MeSH
• ledviny metabolismus   MeSH
• měď metabolismus   otrava   MeSH
• nemoci ovcí chemicky indukované   metabolismus   MeSH
• ovce MeSH
• selen metabolismus   farmakologie   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• látky znečišťující vzduch v pracovním prostředí MeSH
• měď MeSH
• selen MeSH

We studied the dynamics of Cu, Fe, Zn, Mo, As, Cd, Pb and Se in the serum of sheep during experimental copper (Cu) intoxication from emissions from a Cu factory. From the food and the dosed emission, the daily intake was 466.8 mg Cu, 1253.93 mg Fe, 11.67 mg Zn, 0.74 mg Mo, 34.75 mg As, 0.091 mg Cd, 2.13 mg Pb, and 2.57 mg Se/experimental ewe. After the onset of Cu intoxication the first ewe died on the 65th d and the last one on the 84th d of the experiment. Hypercupremia was observed from the 50th d after the beginning of emission administration. Significant changes were observed in serum Cu on the 50th, 70th and 77th d (P less than 0.01). Changes in the dynamics of the other elements were recorded at the end of the cumulative phase and during the hemolytic crisis by also determining Fe, Zn, Mo, As, Cd, Pb and Se in serum of the Cu-poisoned sheep.

• MeSH
• arsen krev   MeSH
• kadmium krev   MeSH
• látky znečišťující vzduch v pracovním prostředí krev   otrava   MeSH
• měď krev   otrava   MeSH
• minerály krev   MeSH
• molybden krev   MeSH
• olovo krev   MeSH
• ovce MeSH
• selen krev   MeSH
• železo krev   MeSH
• zinek krev   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• arsen MeSH
• kadmium MeSH
• látky znečišťující vzduch v pracovním prostředí MeSH
• měď MeSH
• minerály MeSH
• molybden MeSH
• olovo MeSH
• selen MeSH
• železo MeSH
• zinek MeSH

Cu, Fe, Zn, Mo, Se, As, Cd and Pb distribution was followed in the organisms of seven lambing ewes after these animals had been on a diet with industrial contaminants generated by the copper and zinc works. The amount of ingested contaminants per lambing ewe was 31.99 g a day pursuant to the starting liveweight. Per-head daily intake of Cu, Fe, Zn, Mo, Se, As, Cd and Pb in mg: 402.02; 95.97; 6158.07; 1.436; 2.975; 15.38; 0.597; 22.14. The first ewe with symptoms of zinc intoxication died on day 42 and the last on day 58. The highest Zn concentrations were recorded in the dry matter of dead ewe liver (1167.3 +/- 314.1 mg per kg). An amount of 1048 +/- 283.7 mg Zn per kg was cumulated in the kidneys in the process of contaminant administration. High Zn contents were also observed in the uterus and spleen. The highest amounts of Cu were cumulated in liver (445.6 238.1 mg per kg). Spleen was an organ with the highest concentrations of Fe, the ovaries cumulated the highest amounts of Mo and bony tissue and ovaries the highest amounts of Se. An exposure of the organisms to As, Cd and Pb in ewes intoxicated with zinc from a source of industrial pollutants, was reflected in their high contents in the ovaries, kidneys, liver and bony tissue. These results demonstrate that in sheep the liver, kidneys, uterus, spleen and ovaries are the most suitable organs if it is to prove zinc intoxication caused by pollutants from the copper and zinc works. Liver, kidneys, ovaries, uterus, spleen, skeletal muscles and bony tissue seem to be suitable for an evaluation of Cu, Fe, Mo, Se, As, Cd and Pb distribution in the organisms of ewes which died from zinc intoxication caused by pollutants from the above-mentioned source.

• MeSH
• kovy farmakokinetika   MeSH
• látky znečišťující životní prostředí farmakokinetika   MeSH
• ovce metabolismus   MeSH
• tkáňová distribuce MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• kovy MeSH
• látky znečišťující životní prostředí MeSH

The study dealt with the evaluation of the contents of the total serum immunoglobulins (CS-Ig), serum albumin, and with the phagocytic activity of blood leucocytes in trichophytosis-affected calves (aged 4 months), in dependence on the doses (revaccination) of the live avirulent vaccine against trichophytosis (the calf group R) and its administration with a zinc based-injection preparation (calf group ZnR). In calf group ZnR (5 mg Zn.kg-1 l. w.) there was a potentiating effect on the percentual proportion of potential blood phagocytes (compared with data before administration: P less than 0.01 on the first day, P less than 0.05 on the third, eighth and thirtieth day; in comparison with the group of calves R: P less than 0.01 during a 24h experiment) and on the percentage of phagocytic cells (compared with data before administration: P less than 0.05 the first and 30th day; compared with calf group R: P less than 0.05 in a 24h experiment. No effect on the index of phagocytic activity was observed. In both groups positive statistical significance was stated in the level of CS-Ig, as compared with the value before administration (on the 8th, 15th, 30th and 60th day of investigation, P less than 0.05, resp. 0.01), but there was no significance when the groups were compared with each other. Significant increase was stated in serum immunoglobulin concentration on the 15th day of examination within each group of calves. Its concentration in calf group ZnR was lower at the beginning of the experiment and at the end of the experiment it was almost exactly the same as in calf group R.

• MeSH
• fagocytóza * MeSH
• fungální vakcíny aplikace a dávkování   MeSH
• imunoglobuliny analýza   MeSH
• nemoci skotu imunologie   prevence a kontrola   MeSH
• sérový albumin analýza   MeSH
• skot MeSH
• tinea imunologie   prevence a kontrola   veterinární   MeSH
• Trichophyton imunologie   MeSH
• vakcinace veterinární   MeSH
• zinek aplikace a dávkování   MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• fungální vakcíny MeSH
• imunoglobuliny MeSH
• sérový albumin MeSH
• zinek MeSH

The zeolite (clinoptilolite) sorption of arsenic, cadmium, and lead ions from rumen fluid and abomasum juice was investigated in laboratory conditions. Zeolite was found to sorb 91% of lead and 45% of cadmium from rumen fluid in 24 hours. The sorption effectiveness was even higher from abomasum juice where zeolite sorbed 98% lead in 24 hours.

• MeSH
• adsorpce MeSH
• bachor * MeSH
• silikáty hliníku * MeSH
• skot MeSH
• techniky in vitro MeSH
• zeolity MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• silikáty hliníku * MeSH
• zeolity MeSH

The life span and cumulation of heavy metals in the liver of 12 ewes of the Improved Wallachian breed were investigated in relation to feeding pollutants from a copper works. The total intake of copper, iron, zinc, arsenic, cadmium and lead was 466.8; 1253.93; 111.67; 34.75; 0.091; 2.13 mg per experimental animal/day, respectively. The average life span of ewes in the experimental group was 77 days. The difference between the first and the last animal which died from copper intoxication was 18 days. The concentration of copper in the liver of sheep who died from intoxication by this metal (2138.28 +/- 1090.96 mg. kg-1 in dry matter) was significantly higher (p less than 0.01) if compared with the control ewes (212.9 +/- 58.33 mg. .kg-1 in dry matter). In the course of observations no direct dependence between the concentration of copper in the liver and the life span of experimental animals was confirmed. The content of iron and zinc in the liver of experimental animals was within the reference range; the difference in zinc was statistically significant when compared with the control group (p less than 0.01). The intake of pollutants from the copper works increased significantly the concentration of arsenic and lead in the liver of experimental animals (p less than 0.01) if compared with the control group. The amount of cadmium in the liver of experimental ewes was 0.304 +/- 0.22 mg. .kg-1 and in the control ewes 0.285 +/- 0.10 mg.kg-1 in dry matter; no significant difference was determined at the same time.

• MeSH
• játra analýza   MeSH
• kovy analýza   MeSH
• látky znečišťující vzduch v pracovním prostředí otrava   MeSH
• měď otrava   MeSH
• nemoci ovcí chemicky indukované   metabolismus   MeSH
• ovce MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• kovy MeSH
• látky znečišťující vzduch v pracovním prostředí MeSH
• měď MeSH

Thermal stress was induced experimentally in the hybrid pigs LW X L, weighing 45 to 52 kg. The trials were repeated in boxes heated to the temperature of 43 to 47 degrees C. The pigs were examined for the values of tremor, pH, partial pressure of carbon dioxide (pCO2), base excess (BE), buffer base (BB), standard and actual bicarbonate (SB and AB), total carbon dioxide (tCO2) and partial oxygen pressure in blood (pO2). Thermal stress caused marked tachycardia, polypnoea, higher body temperature, increased blood pH, reduction of partial carbon dioxide pressure. Respiratory alkalosis was accompanied by cardiovascular weakness, general excitation followed by apathy, cyanosis and dyspnoea.

• MeSH
• acidobazická rovnováha * MeSH
• prasata metabolismus   MeSH
• vysoká teplota * MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH

If easily digestible saccharides are deficient in the feed ration of bulls with the live weight of 300 kg and at simultaneous single application of urea at a rate of 0.2 g per 1 kg live weight, zeolite (with 50.6% clinoptilolite content) administered at a rate of 2.5% per 1 kg dry matter influenced significantly (P less than 0.05) the ammonia concentration in rumen, v. portae and v. jugularis. The rumen contents and blood were sampled at the intervals of 0, 15, 30, 60, 90, 120, 180 and 360 minutes after feeding. Basal feed ration consisted of 1 kg feed mixture and 3 kg meadow hay. After urea administration, zeolite reduced the ammonia concentration in rumen by 20-40% in comparison with the control group and in v. portae by 60-70%. In v. jugularis in the 90th minute after feeding significant hyperammonemia was observed in bulls with no zeolite supplement. Zeolite administration did not influence urea concentration in plasma.

• MeSH
• amoniak krev   metabolismus   MeSH
• bachor metabolismus   MeSH
• krmivo pro zvířata * MeSH
• močovina krev   metabolismus   MeSH
• silikáty hliníku farmakologie   MeSH
• skot metabolismus   MeSH
• zeolity MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• skot metabolismus   MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• amoniak MeSH
• močovina MeSH
• silikáty hliníku MeSH
• zeolity MeSH

The effect of zeolite (clinoptilolite) on experimentally induced metabolic acidosis was studied in sheep under experimental conditions. Sucrose was administered at the doses of 10 and 15 g per kg l. w. either alone or, in other groups, together with 0.45 g zeolite per kg l. w. The content of volatile fatty acids in rumen contents and the indices of acid-base homeostasis of blood were monitored in the subsequent 24 and/or 48 hours. The examinations showed that simultaneous administration of zeolite with sucrose failed to prevent the rise of metabolic acidosis but the drop in the indices of acid-base homeostasis was less severe, or was delayed by three to six hours. The dose of 0.45 g zeolite per kg l. w. was found to be insufficient for the prevention of a medium-severe or severe course of metabolic acidosis.

• MeSH
• acidóza metabolismus   prevence a kontrola   veterinární   MeSH
• bachor metabolismus   MeSH
• koncentrace vodíkových iontů MeSH
• mastné kyseliny metabolismus   MeSH
• nemoci ovcí metabolismus   prevence a kontrola   MeSH
• ovce MeSH
• silikáty hliníku terapeutické užití   MeSH
• zeolity MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• mastné kyseliny MeSH
• silikáty hliníku MeSH
• zeolity MeSH