γ-Conglutin, a lupin seed protein, is an intriguing protein both in terms of the complexity of its molecular structure and a broad spectrum of unique health-promoting properties manifested in animal and human trials. Moreover, this protein is an evolutionary cornerstone whose physiological significance for the plant has not been determined yet. Herein, a comprehensive characterization of γ-conglutin glycosylation is presented and includes (i) the identification of the N-glycan-bearing site, (ii) the qualitative and quantitative composition of glycan-building saccharides, as well as (iii) the effect of oligosaccharide removal on structural and thermal stability. The obtained results indicate the presence of glycans belonging to different classes attached to the Asn98 residue. In addition, the detachment of the oligosaccharide significantly affects secondary structure composition, which disturbs the oligomerization process. The structural changes were also reflected in biophysical parameters, i.e., at a pH value of 4.5, an increase in γ-conglutin thermal stability was observed for the deglycosylated monomeric form. Collectively, the presented results provide evidence of the high complexity of the post-translational maturation and suggest the possibility of a functional effect that glycosylation might have on γ-conglutin structure integrity.
- Klíčová slova
- N-glycosylation, lupin seed, mass spectrometry, post-translational modification, thermal stability, γ-conglutin,
- MeSH
- glykosylace MeSH
- lidé MeSH
- Lupinus * chemie MeSH
- rostlinné proteiny * metabolismus MeSH
- semena rostlinná chemie MeSH
- zásobní proteiny semen metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- rostlinné proteiny * MeSH
- zásobní proteiny semen MeSH
Old World lupins constitute an interesting model for evolutionary research due to diversity in genome size and chromosome number, indicating evolutionary genome reorganization. It has been hypothesized that the polyploidization event which occurred in the common ancestor of the Fabaceae family was followed by a lineage-specific whole genome triplication (WGT) in the lupin clade, driving chromosome rearrangements. In this study, chromosome-specific markers were used as probes for heterologous fluorescence in situ hybridization (FISH) to identify and characterize structural chromosome changes among the smooth-seeded (Lupinus angustifolius L., Lupinus cryptanthus Shuttlew., Lupinus micranthus Guss.) and rough-seeded (Lupinus cosentinii Guss. and Lupinus pilosus Murr.) lupin species. Comparative cytogenetic mapping was done using FISH with oligonucleotide probes and previously published chromosome-specific bacterial artificial chromosome (BAC) clones. Oligonucleotide probes were designed to cover both arms of chromosome Lang06 of the L. angustifolius reference genome separately. The chromosome was chosen for the in-depth study due to observed structural variability among wild lupin species revealed by BAC-FISH and supplemented by in silico mapping of recently released lupin genome assemblies. The results highlighted changes in synteny within the Lang06 region between the lupin species, including putative translocations, inversions, and/or non-allelic homologous recombination, which would have accompanied the evolution and speciation.
- Klíčová slova
- FISH, chromosome evolution, comparative-mapping, cytogenetics, karyotype evolution, lupin, oligo-painting, oligonucleotide probes, wild species,
- MeSH
- chromozomy rostlin genetika MeSH
- genom rostlinný * MeSH
- hybridizace in situ fluorescenční MeSH
- Lupinus genetika MeSH
- mapování chromozomů * MeSH
- umělé bakteriální chromozomy MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
There is increasing experimental evidence that strigolactones, a class of carotenoid-derived sesquiterpenoid hormones, and their downstream signal components play a role in plant resilience to abiotic stress. Strigolactones positively influence plant coping mechanisms in response to abiotic stressors like drought and high salinity. In this study, we examined the effects of rac-GR24 (a synthetic strigolactone analog) and strigolactone inhibitors on the physiological and molecular responses associated with thermotolerance during seed germination and seedling development in Lupinus angustifolius under heat stress. Photosystem I & II functions were also evaluated via Chl a fluorescence transient analysis in heat stressed lupine seedlings. Our results suggest a putative role for GR24 in mediating tolerance to heat stress during seed germination and seedling development albeit these responses appeared independent of D14-mediated signalling. Seeds primed with GR24 had the highest of all germination indices, enhanced proline content and reduced peroxidation of lipids. GR24 also enhanced the activities of enzymes of the antioxidant and glyoxalase systems in lupine seedlings. The JIP-test indicated that GR24 conferred resistance to heat stress-induced damage to the oxygen evolution complex while also preventing the inactivation of PSII reaction centres thus ensuring PSII thermotolerance.
- Klíčová slova
- Antioxidant enzymes, Chlorophyll a fluorescence, GR24, Germination indices, Glyoxalase system, Heat stress tolerance, JIP-Test, PSII thermotolerance, Strigolactones,
- MeSH
- fotosystém II (proteinový komplex) fyziologie MeSH
- heterocyklické sloučeniny tricyklické farmakologie MeSH
- klíčení * MeSH
- laktony farmakologie MeSH
- Lupinus fyziologie MeSH
- reakce na tepelný šok * MeSH
- semena rostlinná fyziologie MeSH
- semenáček fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fotosystém II (proteinový komplex) MeSH
- GR24 strigolactone MeSH Prohlížeč
- heterocyklické sloučeniny tricyklické MeSH
- laktony MeSH
Lupine seeds have the potential to be an alternative to imported dietary proteins. In rabbits, it has been indicated that White lupine seed (WLS) is a suitable protein source. Other lupine species, for example, narrow-leaved lupine seed (NLS), have not yet been tested in rabbit diets. Two experiments were carried out to evaluate the effect of the dietary inclusion of NLS on growth performance, sanitary risk index (SRI), coefficients of total tract apparent digestibility (CTTAD) and nitrogen output in fattening rabbits. Narrow-leaved lupine was compared with WLS as a main protein source. For Experiment I, a total of 198 Hyplus rabbits (37 days of age) were allocated into two groups (99 rabbits per group), fed the WLS I diet (120 g/kg of WLS cv. Amiga) or the NLS I diet (150 g/kg of NLS cv. Probor), and used for performance and carcass trait evaluations. In addition, the CTTAD of the diets and the nitrogen output were determined in 10 Hyplus rabbits per treatment (37 days of age). For Experiment II, a total of 180 Hyplus rabbits (32 days of age) were allocated into two groups (90 rabbits per group), fed the WLS II diet (120 g/kg of WLS cv. Amiga) or the NLS II diet (130 g/kg of NLS cv. Primadona), and used for performance and carcass trait evaluations. In addition, the CTTAD of the diets was determined in 10 Hyplus rabbits per treatment (32 days of age). Regardless of the treatment, the dietary inclusion of NLS had a negative effect on growth of the rabbits. The nitrogen excretion and coefficients of nitrogen retention of rabbits were not affected by the treatments. In Experiment I, SRI (37 to 80 days of age) was higher in rabbits fed the NLS I diet than in those fed the WLS I diet (38.4% v. 23.2%, respectively; P = 0.031). Similarly, in Experiment II (32 to 74 days of age), SRI was higher in rabbits fed the NLS II diet than in rabbits fed the WLS II diet (37.8% v. 23.3%, respectively; P = 0.052). In conclusion, regardless of the variety, the dietary inclusion of NLS had no negative effect on the nitrogen output or dressing-out percentage of rabbits when compared to those of rabbits fed the WLS diets. With respect to the SRI and performance, however, NLS did not provide a satisfactory outcome.
- Klíčová slova
- growth, health, legumes, nitrogen retention, rabbit,
- MeSH
- dieta veterinární MeSH
- dietní proteiny analýza MeSH
- dusík metabolismus MeSH
- gastrointestinální trakt fyziologie MeSH
- králíci růst a vývoj fyziologie MeSH
- krmivo pro zvířata analýza MeSH
- listy rostlin MeSH
- Lupinus * MeSH
- náhodné rozdělení MeSH
- semena rostlinná MeSH
- trávení MeSH
- zdravotní stav MeSH
- zvířata MeSH
- Check Tag
- králíci růst a vývoj fyziologie MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- dietní proteiny MeSH
- dusík MeSH
This work aimed to extend our current knowledge by obtaining novel information regarding the effect of the dietary inclusion of dehulled white lupine seeds (DWL) on the physical characteristics and sensory qualities of rabbit meat, as well as growth and carcass traits. The DWL diet was compared with a soybean meal-based diet. Feeding the DWL diet to rabbits led to lower Warner-Bratzler shear force values measured in grilled meat samples (Longissimus lumborum muscle) across fibers and this finding was confirmed in the sensory evaluation of texture in terms of higher tenderness and fibrosity of meat samples. No differences were detected by panelists in terms of intensity of aroma, the presence of a flavor typical for cooked meat, or the presence of a flavor typical for rabbit meat. Additionally, saturation and thrombogenic indexes from hind leg meat of rabbits fed the DWL diet were reduced. There was a better feed conversion ratio in rabbits fed the DWL diet.
- Klíčová slova
- Dehulled white lupine, Meat, Physical characteristics, Rabbit, Sensory properties,
- MeSH
- dieta veterinární MeSH
- fyziologie výživy zvířat MeSH
- králíci MeSH
- krmivo pro zvířata analýza MeSH
- Lupinus chemie MeSH
- maso analýza normy MeSH
- semena rostlinná chemie MeSH
- složení těla účinky léků MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- randomizované kontrolované studie MeSH
The effect of dietary inclusion of white lupin seed (WLS) on the milk composition and yield of rabbit does as well as the performance of their litters was studied. Two lactation diets having identical digestible protein (DP):DE ratio and two weaning diets having identical DP:DE ratio were formulated. The first lactation diet (SL) contained soybean meal (SBM; 13.0%) and sunflower meal (5.0%) as the main CP sources, whereas the second lactation diet (LL) was based on WLS (25.0%). As a result, the LL diet had a greater ether extract (EE) content than did the SL diet. The first weaning diet (SW) included SBM (7.0%) as the main CP source, whereas the second weaning diet (LW) diet was based on WLS (12.0%). No additional fat was added to any of the diets. A total of 32 (16 per treatment) Hyplus PS 19 does (4,225 ± 607 g BW, at the second parturition) were fed 1 of the 2 lactation diets. The litters were standardized to 9 kits (564 ± 81 g BW) on the day of birth and were fed 1 of the 2 weaning diets from d 17 to 69 of age. At d 30 of age (weaning), 66 rabbits on each weaning diet (689 ± 71 g BW; 3 per cage) were used to evaluate performance. Feed intake and doe BW were not affected by the dietary treatments. Milk yield tended to be higher between d 1 and 30 of lactation in does fed the LL diet (P = 0.094), a finding that is related to the higher dietary EE content and intake in the LL diet. When expressed per kilogram of metabolic weight, milk output (P < 0.05) and fat output (P < 0.05) were greater in these does. Improved G:F (P < 0.05) between d 1 and 21 of lactation and greater ADG (P = 0.072) and milk efficiency (P < 0.05) of litters was observed in does fed the LL diet. The milk of does fed the LL diet contained less linoleic acid (P < 0.05) and arachidonic acid (C 20:4n-6; P < 0.05) and more oleic acid (P < 0.05), α-linolenic acid (P < 0.05), and eicosapentaenic acid (P < 0.05), with a corresponding increase in the total PUFA n-3:C 20:4n-6 ratio (P < 0.05). The performance of fattening rabbits was not affected by dietary treatment. The number of ill plus dead rabbits caused by digestive disease was lower (P < 0.05) in rabbits fed the LW diet. Therefore, WLS is a suitable dietary CP source for lactating does that can replace traditionally used CP sources without adverse effects on feed intake and milk yield or on the growth and viability of their litters. Due to its fatty acid (FA) composition, the use of WLS in the lactation diet has the potential to improve the milk FA composition of does.
- MeSH
- dieta veterinární MeSH
- fyziologie výživy v mateřství MeSH
- fyziologie výživy zvířat MeSH
- králíci * růst a vývoj fyziologie MeSH
- krmivo pro zvířata analýza MeSH
- laktace účinky léků fyziologie MeSH
- Lupinus * MeSH
- mastné kyseliny chemie metabolismus MeSH
- mléko chemie fyziologie MeSH
- semena rostlinná * MeSH
- zvířata MeSH
- Check Tag
- králíci * růst a vývoj fyziologie MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- mastné kyseliny MeSH
The complete nucleotide sequence of the ssRNA genome of a lupine potyvirus (LP) isolate was determined. It comprised 10,113 nucleotides excluding the poly(A) tail. Phylogenetic analysis of CP protein sequences identified pepper veinal mottle virus, narcissus yellow stripe virus, and chili veinal mottle virus as the closest relatives, sharing coat protein amino acid sequence identities of only about 64% with the LP isolate. Thus, LP can be regarded as a member of a newly described potyvirus species, for which the name Lupine mosaic virus (LuMV) is proposed.
- MeSH
- fylogeneze MeSH
- genom virový MeSH
- Lupinus virologie MeSH
- nemoci rostlin virologie MeSH
- Potyvirus genetika izolace a purifikace MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
A total of 20 weaned rabbits (33 days old) (10 per treatment) were fed one of two diets that included 150 g of sunflower meal (SF)/kg of diet or 120 g of whole white lupin (WL)/kg of diet for 42 days. The WL diet contained less saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA) but more monounsaturated fatty acids (MUFA) than the SF diet. The WL diet significantly decreased SFA and PUFA content, as well as the PUFA n-6/PUFA n-3 ratio and saturation, atherogenic and thrombogenic indexes in hind leg meat. The fatty acid composition in perirenal fat was similar to that of hind leg meat; however, significantly higher MUFA levels were observed in rabbits fed the WL diet. Thus, feeding rabbits the WL diet affected the fatty acid profile of hind leg meat and perirenal fat in a favourable manner.
- MeSH
- ateroskleróza prevence a kontrola MeSH
- dieta veterinární MeSH
- dietní tuky analýza MeSH
- Helianthus MeSH
- kosterní svaly chemie MeSH
- králíci MeSH
- krmivo pro zvířata MeSH
- lidé MeSH
- Lupinus * MeSH
- maso analýza MeSH
- mastné kyseliny analýza MeSH
- nitrobřišní tuk chemie MeSH
- potravní doplňky * MeSH
- semena rostlinná * MeSH
- trombóza prevence a kontrola MeSH
- zdraví MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- dietní tuky MeSH
- mastné kyseliny MeSH
A soil-plant transfer study was performed using soil from a former uranium ore processing factory in South Bohemia. We present the results from greenhouse experiments which include estimates of the time required for phytoremediation. The accumulation of (226)Ra by different plant species from a mixture of garden soil and contaminated substrate was extremely variable, ranging from 0.03 to 2.20 Bq (226)Ra/g DW. We found differences in accumulation of (226)Ra between plants from the same genus and between cultivars of the same plant species. The results of (226)Ra accumulation showed a linear relation between concentration of (226)Ra in plants and concentration of (226)Ra in soil mixtures. On the basis of these results we estimated the time required for phytoremediation, but this appears to be too long for practical purposes.
- MeSH
- biodegradace * MeSH
- Lupinus metabolismus MeSH
- půda analýza MeSH
- radium metabolismus MeSH
- rostliny metabolismus MeSH
- skleníkový efekt MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- půda MeSH
- radium MeSH
- MeSH
- DNA řízené RNA-polymerasy genetika MeSH
- Lupinus virologie MeSH
- molekulární sekvence - údaje MeSH
- nemoci rostlin virologie MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- Potyvirus klasifikace genetika izolace a purifikace MeSH
- sekvenční analýza DNA MeSH
- virové plášťové proteiny genetika MeSH
- virové proteiny genetika MeSH
- Publikační typ
- dopisy MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- DNA řízené RNA-polymerasy MeSH
- virové plášťové proteiny MeSH
- virové proteiny MeSH
