The genus Vibrio is characterized by a large number of species and some of them are human pathogens causing gastrointestinal and wound infections through the ingestion or manipulation of contaminated fishes and shellfish including Vibrio parahaemolyticus and Vibrio alginolyticus. In this study, we reported the phenotypic and molecular characterization of 9 V. parahaemolyticus and 27 V. alginolyticus strains isolated from outbreaks affecting cultured Gilthead sea bream (Sparus aurata L.) and Sea bass (Dicentrarchus labrax) along the Tunisian coast from 2008 to 2009. All isolates were tested for the presence of DNase, caseinase, protease, lipase, amylase, gelatinase, hemolytic activity and antibacterial resistance to different drugs. Randomly amplified polymorphic DNA was used to examine the genetic relatedness among the V. parahaemolyticus and V. alginolyticus strains.

• MeSH
• DNA fingerprinting MeSH
• epidemický výskyt choroby veterinární   MeSH
• fenotyp MeSH
• infekce bakteriemi rodu Vibrio epidemiologie   mikrobiologie   veterinární   MeSH
• mořan zlatý mikrobiologie   MeSH
• nemoci ryb epidemiologie   mikrobiologie   MeSH
• Percoidea mikrobiologie   MeSH
• technika náhodné amplifikace polymorfní DNA metody   MeSH
• Vibrio alginolyticus genetika   izolace a purifikace   MeSH
• Vibrio parahaemolyticus genetika   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Tunisko MeSH

In 1991 a cholera epidemic occurred in Nigeria. The features of this cholera outbreak in a single hospital in Cross River, Nigeria, were examined. Microbiologic techniques included the use of thiosulphate citrate bile-salts sucrose (TCBS) medium for culture of all stool specimens. Vibrio isolates from diarrheic patients included V. cholerae-O1 (75), V. cholerae non-O1 (10) and V. parahaemolyticus (21). The illnesses were diverse, ranging from mild to severe, and in most instances requiring hospitalization, rehydration as well as antibiotic treatment. Eighty patients were hospitalized and six died mainly from hypovolemic shock and acute renal failure arising from excessive fluid loss. The low vibrio-associated mortality observed in this outbreak may have been influenced by the proximity and easy transit access to the health care facilities offered by the teaching hospital. This contrasts with the high mortality figures reported by Health Centers in the rural areas during the same period. Some features of vibrio diarrheas were comparable with those of other enteric pathogens. Poorly developed water and sewage disposal systems, contact with sea water, consumption of fishery products and leftover foods were the main risk factors identified.

• MeSH
• cholera farmakoterapie   epidemiologie   mikrobiologie   přenos   MeSH
• dítě MeSH
• dospělí MeSH
• epidemický výskyt choroby * MeSH
• gastroenteritida farmakoterapie   epidemiologie   mikrobiologie   MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• mikrobiologické techniky MeSH
• mladiství MeSH
• potravinářská mikrobiologie MeSH
• předškolní dítě MeSH
• průjem farmakoterapie   epidemiologie   mikrobiologie   MeSH
• rizikové faktory MeSH
• sanitace MeSH
• senioři MeSH
• těhotenství MeSH
• Vibrio cholerae účinky léků   izolace a purifikace   patogenita   MeSH
• Vibrio parahaemolyticus účinky léků   izolace a purifikace   patogenita   MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• senioři MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Nigérie epidemiologie   MeSH

Seashore water samples collected along the coastline in Bulgaria and Rumania contained in large numbers OK serovars of V. parahaemolyticus; some of these had been isolated repeatedly over an extended time period: 01 K32, 03 K30, 03 K48, 04 K37, 04 K53, 05 K17, 05 K30. The serovar 05 K17 was virtually present in all water samples and was also isolated from a case of purulent ear infection in a child from Burgas. In contrast, strains recovered from Asian and African coastal water had different K antigens and were never identified in Europe. Two strains of V. parahaemolyticus (serovars 05 K15 and 07 K10) had positive swarming growth resembling that of V. alginolyticus. The first of these was Kanagawa-positive and was isolated from a case of severe diarrhea in Brazzaville. Vibrio parahaemolyticus isolates came from marine or brackish water specimens collected on sand banks, 3 strains were recovered from marine or brackish water in Africa. Vibrio harveyi, a sucrose-negative species important from differential diagnostic aspects, has been isolated from seashore water samples collected on coarse-sand or pebbly beaches.

• MeSH
• feces mikrobiologie   MeSH
• gastroenteritida diagnóza   MeSH
• infekce bakteriemi rodu Vibrio diagnóza   MeSH
• lidé MeSH
• mikrobiologie vody MeSH
• mořská voda MeSH
• ryby mikrobiologie   MeSH
• sérotypizace MeSH
• Vibrio parahaemolyticus klasifikace   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• gastroenteritida mikrobiologie   MeSH
• lidé MeSH
• mikrobiologie vody * MeSH
• mořská voda * MeSH
• Vibrio parahaemolyticus izolace a purifikace   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

At total of 15 subjects taken ill with food poisoining caused by parahaemolytic and NAG vibrios after eating boiled pork sausage and boiled veal stomach were examined. The meat products were secondarily contamined in the sausage factory due to the use od non-disinfected lake water which was found to be a natural medium of the vibrios. When the use of this water was prohibited, the contamination of the factory and the meat products disappeared and no further cases of food poisoning due to meat products were notified. The disease has a toxic character, runs a bening course and end after 2--3 days by restoration to full health.

• MeSH
• časové faktory MeSH
• infekce bakteriemi rodu Vibrio etiologie   MeSH
• látky znečišťující vodu analýza   MeSH
• lidé MeSH
• masný průmysl MeSH
• maso škodlivé účinky   MeSH
• nemoci přenášené potravou etiologie   MeSH
• Vibrio parahaemolyticus izolace a purifikace   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• látky znečišťující vodu MeSH

At total of 15 subjects taken ill with food poisoining caused by parahaemolytic and NAG vibrios after eating boiled pork sausage and boiled veal stomach were examined. The meat products were secondarily contamined in the sausage factory due to the use od non-disinfected lake water which was found to be a natural medium of the vibrios. When the use of this water was prohibited, the contamination of the factory and the meat products disappeared and no further cases of food poisoning due to meat products were notified. The disease has a toxic character, runs a bening course and ends after 2--3 days by restoration to full health.

• MeSH
• feces mikrobiologie   MeSH
• kontaminace potravin MeSH
• lidé MeSH
• maso MeSH
• nemoci přenášené potravou etiologie   MeSH
• potravinářská mikrobiologie MeSH
• Vibrio parahaemolyticus izolace a purifikace   MeSH
• Vibrio izolace a purifikace   MeSH
• znečištění vody MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Vibrio parahaemolyticus was isolated in two subjects with acute gastroenteritis. The patients' history included stay in Pakistan or India. They probably contracted the infection on board an aeroplane where they ingested cold meal containing sea products. The possibility of this conditioned pathogen occurring also in an inland country is pointed out.

• MeSH
• akutní nemoc MeSH
• dospělí MeSH
• gastroenteritida etiologie   MeSH
• infekce bakteriemi rodu Vibrio etiologie   MeSH
• kontaminace potravin MeSH
• lidé MeSH
• měkkýši, korýši MeSH
• Vibrio parahaemolyticus izolace a purifikace   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• Geografické názvy
• Československo MeSH

Vibrio parahaemolyticus was isolated in two subjects with acute gastroenteritis. The patients history included stay in Pakistan or India. They probably contracted the infection on board an aeroplane where they ingested cold meal containing sea products. The possibility of this conditioned pathogen occurring also in an inland country is pointed out.

• MeSH
• dospělí MeSH
• infekce bakteriemi rodu Vibrio etiologie   MeSH
• lidé MeSH
• Vibrio parahaemolyticus * MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Československo MeSH