OBJECTIVE: The aim of this study was to: (1) evaluate the anti-inflammatory effects of cannabidiol (CBD) on primary cultures of human gingival fibroblasts (HGFs) and (2) to clinically monitor the effect of CBD in subjects with periodontitis. BACKGROUND: The use of phytocannabinoids is a new approach in the treatment of widely prevalent periodontal disease. MATERIALS AND METHODS: Cannabinoid receptors were analyzed by western blot and interleukin production detected using enzyme immunoassay. Activation of the Nrf2 pathway was studied via monitoring the mRNA level of heme oxygenase-1. Antimicrobial effects were determined by standard microdilution and 16S rRNA screening. In the clinical part, a placebo-control double-blind randomized study was conducted (56 days) in three groups (n = 90) using dental gel without CBD (group A) and with 1% (w/w) CBD (group B) and corresponding toothpaste (group A - no CBD, group B - with CBD) for home use to maintain oral health. Group C used dental gel containing 1% chlorhexidine digluconate (active comparator) and toothpaste without CBD. RESULTS: Human gingival fibroblasts were confirmed to express the cannabinoid receptor CB2. Lipopolysaccharide-induced cells exhibited increased production of pro-inflammatory IL-6 and IL-8, with deceasing levels upon exposure to CBD. CBD also exhibited antimicrobial activities against Porphyromonas gingivalis, with an MIC of 1.5 μg/mL. Activation of the Nrf2 pathway was also demonstrated. In the clinical part, statistically significant improvement was found for the gingival, gingival bleeding, and modified gingival indices between placebo group A and CBD group B after 56 days. CONCLUSIONS: Cannabidiol reduced inflammation and the growth of selected periodontal pathogenic bacteria. The clinical trial demonstrated a statistically significant improvement after CBD application. No adverse effects of CBD were reported by patients or observed upon clinical examination during the study. The results are a promising basis for a more comprehensive investigation of the application of non-psychotropic cannabinoids in dentistry.

• Klíčová slova
• cannabidiol, inflammation, microbiota, oral hygiene, periodontium, phytocannabinoid,
• MeSH
• antiflogistika terapeutické užití   farmakologie   MeSH
• chlorhexidin terapeutické užití   farmakologie   analogy a deriváty   MeSH
• dospělí MeSH
• dvojitá slepá metoda MeSH
• faktor 2 související s NF-E2 MeSH
• fibroblasty * účinky léků   MeSH
• gingiva * účinky léků   MeSH
• gingivitida * farmakoterapie   MeSH
• hemoxygenasa-1 MeSH
• interleukin-6 analýza   MeSH
• interleukin-8 účinky léků   MeSH
• kanabidiol * farmakologie   terapeutické užití   MeSH
• kultivované buňky MeSH
• lidé středního věku MeSH
• lidé MeSH
• parodontitida farmakoterapie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• antiflogistika MeSH
• chlorhexidin MeSH
• chlorhexidine gluconate MeSH Prohlížeč
• faktor 2 související s NF-E2 MeSH
• hemoxygenasa-1 MeSH
• interleukin-6 MeSH
• interleukin-8 MeSH
• kanabidiol * MeSH
• NFE2L2 protein, human MeSH Prohlížeč

BACKGROUND: Plaque-induced gingivitis is the most prevalent periodontal disease associated with pathogenic biofilms. The host immune system responds to pathogens through pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs) and their co-receptor cluster of differentiation 14 (CD14). AIM: This study investigated the association between the functional polymorphism in the CD14 gene and the dental plaque microbiota in children with gingivitis. DESIGN: A total of 590 unrelated children (307 with plaque-induced gingivitis and 283 controls, aged 13-15 years) were enrolled in this case-control study. Dental plaque was processed using a ParoCheck® 20 detection kit. The CD14 -260C/T (rs2569190) polymorphism was determined with the PCR-RFLP method. RESULTS: Gingivitis was detected in 64.2% of boys and 35.8% of girls (P < .001). Children with gingivitis had a significantly higher occurrence of dental caries (P < .001). No significant differences in the CD14 -260C/T allele and genotype distribution among individuals with or without gingivitis in the whole cohort were found. Children with gingivitis and P gingivalis, however, were significantly more frequent carriers of the CT and TT genotypes than children with gingivitis without P gingivalis or healthy controls (P < .05). CONCLUSIONS: The CD14 -260C/T polymorphism acts in cooperation with P gingivalis to trigger plaque-induced gingivitis in Czech children.

• Klíčová slova
• CD14, bacteria, gingivitis, polymorphism,
• MeSH
• dítě MeSH
• gingivitida * genetika   MeSH
• lidé MeSH
• lipopolysacharidové receptory * MeSH
• mladiství MeSH
• polymorfismus genetický MeSH
• Porphyromonas gingivalis MeSH
• studie případů a kontrol MeSH
• zubní kaz * MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• CD14 protein, human MeSH Prohlížeč
• lipopolysacharidové receptory * MeSH

Oral health is important not only due to the diseases emerging in the oral cavity but also due to the direct relation to systemic health. Thus, early and accurate characterization of the oral health status is of utmost importance. There are several salivary biomarkers as candidates for gingivitis and periodontitis, which are major oral health threats, affecting the gums. These need to be verified and validated for their potential use as differentiators of health, gingivitis and periodontitis status, before they are translated to chair-side for diagnostics and personalized monitoring. We aimed to measure 10 candidates using high sensitivity ELISAs in a well-controlled cohort of 127 individuals from three groups: periodontitis (60), gingivitis (31) and healthy (36). The statistical approaches included univariate statistical tests, receiver operating characteristic curves (ROC) with the corresponding Area Under the Curve (AUC) and Classification and Regression Tree (CART) analysis. The main outcomes were that the combination of multiple biomarker assays, rather than the use of single ones, can offer a predictive accuracy of > 90% for gingivitis versus health groups; and 100% for periodontitis versus health and periodontitis versus gingivitis groups. Furthermore, ratios of biomarkers MMP-8, MMP-9 and TIMP-1 were also proven to be powerful differentiating values compared to the single biomarkers.

• MeSH
• biologické markery metabolismus   MeSH
• dospělí MeSH
• ELISA metody   MeSH
• gingivitida diagnóza   metabolismus   MeSH
• kohortové studie MeSH
• lidé středního věku MeSH
• lidé MeSH
• matrixová metaloproteinasa 8 metabolismus   MeSH
• matrixová metaloproteinasa 9 metabolismus   MeSH
• orální zdraví * MeSH
• parodontitida diagnóza   metabolismus   MeSH
• plocha pod křivkou MeSH
• průřezové studie MeSH
• ROC křivka MeSH
• sliny metabolismus   MeSH
• studie případů a kontrol MeSH
• tkáňový inhibitor metaloproteinasy 1 metabolismus   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické markery MeSH
• matrixová metaloproteinasa 8 MeSH
• matrixová metaloproteinasa 9 MeSH
• MMP8 protein, human MeSH Prohlížeč
• MMP9 protein, human MeSH Prohlížeč
• TIMP1 protein, human MeSH Prohlížeč
• tkáňový inhibitor metaloproteinasy 1 MeSH

AIM: We analyzed the VDR TaqI (rs731236) gene polymorphism in children with and those without dental caries. METHODS: A total of 388 subjects, 153 caries-free (with decayed/missing/filled teeth [DMFT] = 0) and 235 children with dental caries (DMFT ≥1), were genotyped by the TaqMan method. RESULTS: Although no significant differences in VDR TaqI allele and genotype frequencies between caries-free and caries-affected children were detected, a significant association between this polymorphism and gingivitis was found (p < 0.05). CONCLUSIONS: In contrast to previous studies from China and Turkey, the VDR TaqI gene variant cannot be used as a marker for identification of Czech children with increased dental caries risk.

• MeSH
• alely MeSH
• DMF Index MeSH
• genetická predispozice k nemoci genetika   MeSH
• genetické asociační studie MeSH
• genotyp MeSH
• gingivitida epidemiologie   genetika   MeSH
• jednonukleotidový polymorfismus * MeSH
• lidé MeSH
• mladiství MeSH
• odds ratio MeSH
• receptory kalcitriolu genetika   MeSH
• restrikční endonukleasy typu II MeSH
• studie případů a kontrol MeSH
• zubní kaz epidemiologie   genetika   MeSH
• Check Tag
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH
• Názvy látek
• receptory kalcitriolu MeSH
• restrikční endonukleasy typu II MeSH
• TCGA-specific type II deoxyribonucleases MeSH Prohlížeč
• VDR protein, human MeSH Prohlížeč

Our ability of screening broad communities for clinically asymptomatic diseases critically drives population health. Sensory chewing gums are presented targeting the tongue as 24/7 detector allowing diagnosis by "anyone, anywhere, anytime". The chewing gum contains peptide sensors consisting of a protease cleavable linker in between a bitter substance and a microparticle. Matrix metalloproteinases in the oral cavity, as upregulated in peri-implant disease, specifically target the protease cleavable linker while chewing the gum, thereby generating bitterness for detection by the tongue. The peptide sensors prove significant success in discriminating saliva collected from patients with peri-implant disease versus clinically asymptomatic volunteers. Superior outcome is demonstrated over commercially available protease-based tests in saliva. "Anyone, anywhere, anytime" diagnostics are within reach for oral inflammation. Expanding this platform technology to other diseases in the future features this diagnostic as a massive screening tool potentially maximizing impact on population health.Early detection of gum inflammation caused by dental implants helps prevent tissue damage. Here, the authors present a peptide sensor that generates a bitter taste when cleaved by proteases present in peri-implant disease, embed it in a chewing gum, and compare the probe to existing sensors using patient saliva.

• MeSH
• chuť * MeSH
• gingivitida diagnóza   metabolismus   MeSH
• lidé MeSH
• matrixové metaloproteinasy metabolismus   MeSH
• parodontitida diagnóza   metabolismus   MeSH
• peptidy metabolismus   MeSH
• sliny enzymologie   MeSH
• zubní implantáty * MeSH
• žvýkačka * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• matrixové metaloproteinasy MeSH
• peptidy MeSH
• zubní implantáty * MeSH
• žvýkačka * MeSH

BACKGROUND: Human immunodeficiency virus (HIV) continues to be a serious health issue and one of the world most devastating epidemics. An estimated 1.5 million people died from AIDS-related illnesses in 2013, and an estimated 37 million people with AIDS have died worldwide since the epidemic has begun. HIV infection is known for its oral manifestations which causes discomfort and pain for infected individuals. The objective of this study was to document oral conditions of HIV positive patients and the pattern and frequency of oral and dental lesions. METHODS: All patients with confirmed HIV infection who were treated at the Department of Dentistry, University Hospital in Hradec Králové, were examined. RESULTS: During the study period, 29 HIV positive patients were examined and treated--19 men, 10 women, with mean age of 32.9 years (range 22-58 years). 72.41% patients received ART. In total, all patients underwent 186 visits. The most frequent treatments were associated with teeth and periodontal lesions (71.80%), oral mucosal lesions were diagnosed and treated only in 3.96% cases. CONCLUSION: Since the introduction of ART, the frequency of oral mucosal lesions is minimal in patients with HIV infection.

• Klíčová slova
• Dental lesions, HIV, Oral mucosal lesions,
• MeSH
• antiretrovirové látky terapeutické užití   MeSH
• dospělí MeSH
• gingivitida epidemiologie   MeSH
• HIV infekce farmakoterapie   epidemiologie   MeSH
• Kaposiho sarkom epidemiologie   MeSH
• kohortové studie MeSH
• lichen planus orální epidemiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• nádory úst epidemiologie   MeSH
• nekróza zubní dřeně epidemiologie   MeSH
• nemoci úst epidemiologie   MeSH
• nemoci zubů epidemiologie   MeSH
• orální kandidóza epidemiologie   MeSH
• parodontitida epidemiologie   MeSH
• pulpitida epidemiologie   MeSH
• sialoadenitida epidemiologie   MeSH
• stomatitida herpetická epidemiologie   MeSH
• vředy v ústech epidemiologie   MeSH
• zubní kaz epidemiologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH
• Názvy látek
• antiretrovirové látky MeSH

CRP is a plasma protein that reflects a measure of the acute phase response to inflammation and is one of the markers of choice in monitoring this response. CRP can be used for the prediction and early detection of periodontal disease. The aim of this study was to compare and evaluate the systemic levels of CRP in the peripheral blood samples of patients with chronic and aggressive periodontitis, gingivitis, and gingival recessions and compare them with periodontal clinical parameters. All patients (N = 158) were examined prior to the initiation of periodontal treatment. Patients were divided into four groups. Group A consisted of 26 patients with aggressive periodontitis, Group B consisted of 111 patients with chronic periodontitis, Group C consisted of 13 patients with gingivitis, and Group D consisted of 8 patients with gingival recessions. Our study results indicate that CRP levels increase subsequently with the severity of the periodontal disease and that the bleeding on probing index showed much better positive correlation with the CRP levels compared to the pocket depth index in both periodontitis patients groups, especially in aggressive periodontitis patients.

• MeSH
• agresivní parodontitida metabolismus   MeSH
• C-reaktivní protein metabolismus   MeSH
• chronická parodontitida metabolismus   MeSH
• dospělí MeSH
• gingivitida metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• ústup dásní metabolismus   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• C-reaktivní protein MeSH

The aim of the study was to test the hypothesis that oral plasma cell granuloma may represent a mucosal manifestation of immunoglobulin (Ig)G4-related disease (IgG4-RD) in the oral cavity. The study sample comprised two males and four females, aged 54-79 years (median 62 years). The lesions were localized on gingival/alveolar mucosa (four cases), hard palate, and floor of the mouth, measuring 17-40 mm (median 31 mm). The duration of the lesions ranged from 3 months to several years. Information on IgG4 serum levels was available for two patients, and these were increased to 1.85 and 1.65 g/L, respectively. The follow-up period ranged 11-30 months (median 13 months). None of the lesions recurred, and none of the patients developed any manifestation of IgG4-RD. Microscopically, all cases presented as nodular lesions composed of numerous polyclonal plasma cells admixed with lymphocytes, histiocytes, mast cells, and eosinophils, set within collagenized stroma in variable proportions. Obliterative phlebitis was observed in two cases. The number of IgG4-positive plasma cells ranged between 51 and 142 per HPF (median 114), while the IgG4/IgG ratio values ranged between 0.16 and 0.72 (median 0.44) and were above 0.40 in three cases. Based on international criteria, two cases were diagnosed as definite and one as probable IgG4-RD. Oral plasma cell granuloma is a heterogeneous group of lesions, and a subset may represent a mucosal manifestation of IgG4-RD in the oral cavity.

• MeSH
• časové faktory MeSH
• diferenciální diagnóza MeSH
• eozinofily patologie   MeSH
• gingivitida diagnóza   imunologie   patologie   MeSH
• histiocyty patologie   MeSH
• imunoglobulin G metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mastocyty patologie   MeSH
• nemoci imunitního systému diagnóza   imunologie   patologie   MeSH
• nemoci úst diagnóza   imunologie   patologie   MeSH
• plazmatické buňky patologie   MeSH
• plazmocelulární granulom diagnóza   imunologie   patologie   MeSH
• senioři MeSH
• ústní sliznice imunologie   patologie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• imunoglobulin G MeSH

BACKGROUND: Langerhans cell histiocytosis is a rare disease characterized by monoclonal proliferation and migration of special dendritic cells. This disease primarily affects bones, but occurs less frequently in other organ systems or may manifest as a multisystem disease. CASE REPORTS: Extraoral and intraoral symptoms of Langerhans cell histiocytosis are described in a 13-month-old female and a 5-month-old male infant. Dermatitis was found on the scalp, abdomen, flexures and in intertrigineous areas in both patients. The intraoral examination of the 13-month-old infant showed premature eruption of all maxillary deciduous molars, loosening and significant damage of periodontal tissues (gingivitis with bleeding, swelling of palatal mucosa, periodontal pockets) resembling severe periodontitis. In the oral cavity of the 5-month-old predentate infant bilateral swellings of maxillary alveolar mucosa with deep ulcerations were seen. The oral and skin symptoms in both infants were indications for biopsy. Langerhans cell histiocytosis was confirmed histologically and immunohistochemically. CONCLUSION: Oral findings in Langerhans cell histiocytosis may be the only clinical symptom of the disease; therefore the role of dentists in establishing diagnosis is very important.

• Klíčová slova
• Bone lesions, Dermatitis, Granulomatous gingivitis, Infants, Langerhans cell histiocytosis, Periodontitis,
• MeSH
• biopsie MeSH
• dermatitida diagnóza   MeSH
• dermatózy skalpu diagnóza   MeSH
• diferenciální diagnóza MeSH
• gingivitida diagnóza   MeSH
• histiocytóza z Langerhansových buněk diagnóza   MeSH
• kojenec MeSH
• krvácení dásní diagnóza   MeSH
• lidé MeSH
• moláry patofyziologie   MeSH
• následné studie MeSH
• nemoci úst diagnóza   MeSH
• parodontální chobot diagnóza   MeSH
• parodontitida diagnóza   MeSH
• prořezávání zubů fyziologie   MeSH
• stomatitida diagnóza   MeSH
• vředy v ústech diagnóza   MeSH
• zuby mléčné patofyziologie   MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• přehledy MeSH

OBJECTIVES: The aim of the present cross-sectional study was to assess oral health in adolescents selected from the ELSPAC (European Longitudinal Study of Pregnancy and Childhood) Bmo group and complete thus the ELSPAC series of studies on child general health. MATERIAL AND METHODS: Randomly selected children from the ELSPAC group (n=780) were examined clinically for dental and periodontal status, dental plaque, dental calculus and orthodontic anomalies. The following clinical parameters were assessed: DMFT score and its components, gingival index (GI), plaque index (PI) and calculus index (CSI). GI, PI and CSI were recorded on selected teeth. The presence/absence of orthodontic anomalies and their severity were recorded. ANOVA test for quantitative and XZ2 test for qualitative parameters evaluation were used. RESULTS: Mean DMFT of the group was 2.82 (SE 0.36), share of caries-free children 25.4%. Mean GI index of the cohort was 0.204 (SE 0.011), grade 0 was found in 36.9% children, grade 1 in 43.0%, and grade 2 in 19.5%. Statistical significant associations (p < 0.05) were observed in GI and DMFT, GI and DT value, GI and severity of orthodontic anomaly; significant difference was found in GI of caries-free and treated children vs. treatment need and in PI value between children with gingivitis vs healthy ones. CONCLUSION: The results demonstrated a relatively high caries experience, low level of gingival inflammation and relation between GI and DMFT, particularly in D component, and between GI and orthodontic anomalies.

• MeSH
• DMF Index MeSH
• gingivitida epidemiologie   MeSH
• index orální hygieny MeSH
• index potřeby ortodontické léčby statistika a číselné údaje   MeSH
• indexy plaku MeSH
• kohortové studie MeSH
• lidé MeSH
• longitudinální studie MeSH
• malokluze epidemiologie   MeSH
• mladiství MeSH
• nemoci parodontu epidemiologie   MeSH
• odhad potřeb statistika a číselné údaje   MeSH
• parodontální index MeSH
• prospektivní studie MeSH
• průřezové studie MeSH
• zubní kámen epidemiologie   MeSH
• zubní kaz epidemiologie   MeSH
• zubní plak epidemiologie   MeSH
• Check Tag
• lidé MeSH
• mladiství MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH