BACKGROUND: Oral squamous cell carcinoma (OSCC) severely affects the quality of life and the 5-year survival rate is low. Exploring the potential miRNA-mRNA regulatory network and analyzing hub genes and clinical data can provide a theoretical basis for further elucidating the pathogenesis of OSCC. METHODS: The miRNA expression datasets of GSE113956 and GSE124566 and mRNA expression datasets of GSE31056, GSE37991 and GSE13601 were obtained from the Gene Expression Omnibus databases. The differentially expressed miRNAs (DEMs) and mRNAs (DEGs) were screened using GEO2R. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed by DAVID database. The PPI network was established through STRING database and the hub genes were preliminarily screened out by Cytoscape software. After identifying the hub genes in the TCGA database, we predicted the potential DEM transcription factors, constructed a miRNA-mRNA regulatory network, and analyzed the relationship between the hub genes and clinical data. RESULTS: A total of 28 DEMs and 764 DEGs were screened out, which were composed of 285 up-regulated genes and 479 down-regulated genes. Enrichment analysis showed that up-regulation of DEGs were mainly enriched in extracellular matrix organization and cancer-related pathway, while down-regulation of DEGs were mainly enriched in muscular system process and adrenaline signal transduction. After preliminary screening by PPI network and identification in TCGA, the up-regulated FN1, COL1A1, COL1A2, AURKA, CCNB1, CCNA2, SPP1, CDC6, and down-regulated ACTN2, TTN, IGF1, CAV3, MYL2, DMD, LDB3, CSRP3, ACTA1, PPARG were identified as hub genes. The miRNA-mRNA regulation network showed that hsa-miR-513b was the DEM with the most regulation, and COL1A1 was the DEG with the most regulation. In addition, CDC6, AURKA, CCNB1 and CCNA2 were related to overall survival and tumor differentiation. CONCLUSIONS: The regulatory relationship of hsa-miR-513b/ CDC6, CCNB1, CCNA2 and the regulatory relationship of hsa-miR-342-5p /AURKA were not only verified in the miRNA-mRNA regulatory network but also related to overall survival and tumor differentiation. These results indicated that they participated in the cellular regulatory process, and provided a molecular mechanism model for the study of pathogenesis.
- Klíčová slova
- bioinformatics, oral squamous cell carcinoma (OSCC), regulatory network,
- MeSH
- adrenalin MeSH
- aurora kinasa A genetika metabolismus MeSH
- dlaždicobuněčné karcinomy hlavy a krku * genetika MeSH
- genové regulační sítě MeSH
- kvalita života MeSH
- lidé MeSH
- messenger RNA genetika metabolismus MeSH
- mikro RNA * genetika MeSH
- nádory úst * genetika MeSH
- PPAR gama genetika metabolismus MeSH
- regulace genové exprese u nádorů MeSH
- stanovení celkové genové exprese MeSH
- transkripční faktory genetika MeSH
- výpočetní biologie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- adrenalin MeSH
- aurora kinasa A MeSH
- messenger RNA MeSH
- mikro RNA * MeSH
- PPAR gama MeSH
- transkripční faktory MeSH
Primary cilia play critical roles in development and disease. Their assembly and disassembly are tightly coupled to cell cycle progression. Here, we present data identifying KIF14 as a regulator of cilia formation and Hedgehog (HH) signaling. We show that RNAi depletion of KIF14 specifically leads to defects in ciliogenesis and basal body (BB) biogenesis, as its absence hampers the efficiency of primary cilium formation and the dynamics of primary cilium elongation, and disrupts the localization of the distal appendage proteins SCLT1 and FBF1 and components of the IFT-B complex. We identify deregulated Aurora A activity as a mechanism contributing to the primary cilium and BB formation defects seen after KIF14 depletion. In addition, we show that primary cilia in KIF14-depleted cells are defective in response to HH pathway activation, independently of the effects of Aurora A. In sum, our data point to KIF14 as a critical node connecting cell cycle machinery, effective ciliogenesis, and HH signaling.
- MeSH
- adaptorové proteiny signální transdukční metabolismus MeSH
- aurora kinasa A antagonisté a inhibitory genetika metabolismus MeSH
- bazální tělíska metabolismus MeSH
- buněčný cyklus genetika MeSH
- chromatografie kapalinová MeSH
- cilie genetika metabolismus patologie MeSH
- HEK293 buňky MeSH
- interfáze fyziologie MeSH
- intracelulární signální peptidy a proteiny genetika metabolismus MeSH
- kineziny genetika metabolismus MeSH
- lidé MeSH
- mitóza genetika MeSH
- onkogenní proteiny genetika metabolismus MeSH
- protein-serin-threoninkinasy genetika metabolismus MeSH
- proteiny hedgehog metabolismus MeSH
- RNA interference MeSH
- signální transdukce genetika MeSH
- sodíkové kanály metabolismus MeSH
- tandemová hmotnostní spektrometrie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adaptorové proteiny signální transdukční MeSH
- AURKA protein, human MeSH Prohlížeč
- aurora kinasa A MeSH
- citron-kinase MeSH Prohlížeč
- FBF1 protein, human MeSH Prohlížeč
- intracelulární signální peptidy a proteiny MeSH
- KIF14 protein, human MeSH Prohlížeč
- kineziny MeSH
- onkogenní proteiny MeSH
- protein-serin-threoninkinasy MeSH
- proteiny hedgehog MeSH
- SCLT1 protein, human MeSH Prohlížeč
- sodíkové kanály MeSH
Errors in chromosome segregation during female meiosis I occur frequently, and aneuploid embryos account for 1/3 of all miscarriages in humans [1]. Unlike mitotic cells that require two Aurora kinase (AURK) homologs to help prevent aneuploidy (AURKA and AURKB), mammalian germ cells also require a third (AURKC) [2, 3]. AURKA is the spindle-pole-associated homolog, and AURKB/C are the chromosome-localized homologs. In mitosis, AURKB has essential roles as the catalytic subunit of the chromosomal passenger complex (CPC), regulating chromosome alignment, kinetochore-microtubule attachments, cohesion, the spindle assembly checkpoint, and cytokinesis [4, 5]. In mouse oocyte meiosis, AURKC takes over as the predominant CPC kinase [6], although the requirement for AURKB remains elusive [7]. In the absence of AURKC, AURKB compensates, making defining potential non-overlapping functions difficult [6, 8]. To investigate the role(s) of AURKB and AURKC in oocytes, we analyzed oocyte-specific Aurkb and Aurkc single- and double-knockout (KO) mice. Surprisingly, we find that double KO female mice are fertile. We demonstrate that, in the absence of AURKC, AURKA localizes to chromosomes in a CPC-dependent manner. These data suggest that AURKC prevents AURKA from localizing to chromosomes by competing for CPC binding. This competition is important for adequate spindle length to support meiosis I. We also describe a unique requirement for AURKB to negatively regulate AURKC to prevent aneuploidy. Together, our work reveals oocyte-specific roles for the AURKs in regulating each other's localization and activity. This inter-kinase regulation is critical to support wild-type levels of fecundity in female mice.
- Klíčová slova
- Aurora kinase, aneuploidy, fertility, meiosis, meiotic maturation, oocyte,
- MeSH
- aneuploidie MeSH
- aurora kinasa A genetika metabolismus MeSH
- aurora kinasa B genetika metabolismus MeSH
- aurora kinasa C genetika metabolismus MeSH
- fertilita genetika MeSH
- meióza * MeSH
- myši MeSH
- oocyty metabolismus MeSH
- segregace chromozomů genetika MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Názvy látek
- Aurka protein, mouse MeSH Prohlížeč
- Aurkb protein, mouse MeSH Prohlížeč
- Aurkc protein, mouse MeSH Prohlížeč
- aurora kinasa A MeSH
- aurora kinasa B MeSH
- aurora kinasa C MeSH
The mitotic kinase Aurora-A and its partner protein TPX2 (Targeting Protein for Xenopus kinesin-like protein 2) are overexpressed in cancers, and it has been proposed that they work together as an oncogenic holoenzyme. TPX2 is responsible for activating Aurora-A during mitosis, ensuring proper cell division. Disruption of the interface with TPX2 is therefore a potential target for novel anticancer drugs that exploit the increased sensitivity of cancer cells to mitotic stress. Here, we investigate the interface using coprecipitation assays and isothermal titration calorimetry to quantify the energetic contribution of individual residues of TPX2. Residues Tyr8, Tyr10, Phe16, and Trp34 of TPX2 are shown to be crucial for robust complex formation, suggesting that the interaction could be abrogated through blocking any of the three pockets on Aurora-A that complement these residues. Phosphorylation of Aurora-A on Thr288 is also necessary for high-affinity binding, and here we identify arginine residues that communicate the phosphorylation of Thr288 to the TPX2 binding site. With these findings in mind, we conducted a high-throughput X-ray crystallography-based screen of 1255 fragments against Aurora-A and identified 59 hits. Over three-quarters of these hits bound to the pockets described above, both validating our identification of hotspots and demonstrating the druggability of this protein-protein interaction. Our study exemplifies the potential of high-throughput crystallography facilities such as XChem to aid drug discovery. These results will accelerate the development of chemical inhibitors of the Aurora-A/TPX2 interaction.
- MeSH
- aurora kinasa A chemie metabolismus MeSH
- jaderné proteiny chemie metabolismus MeSH
- krystalografie rentgenová MeSH
- lidé MeSH
- ligandy MeSH
- mapy interakcí proteinů účinky léků MeSH
- objevování léků MeSH
- proteiny asociované s mikrotubuly chemie metabolismus MeSH
- proteiny buněčného cyklu chemie metabolismus MeSH
- simulace molekulového dockingu MeSH
- thiazolidiny chemie farmakologie MeSH
- vazba proteinů účinky léků MeSH
- vazebná místa účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aurora kinasa A MeSH
- jaderné proteiny MeSH
- ligandy MeSH
- proteiny asociované s mikrotubuly MeSH
- proteiny buněčného cyklu MeSH
- thiazolidiny MeSH
- TPX2 protein, human MeSH Prohlížeč
Aurora-A kinase (AURKA), a member of the serine/threonine protein kinase family, is involved in multiple steps of mitotic progression. It regulates centrosome maturation, mitotic spindle formation, and cytokinesis. While studied extensively in somatic cells, little information is known about AURKA in the early cleavage mouse embryo with respect to acentrosomal spindle assembly. In vitro experiments in which AURKA was inactivated with specific inhibitor MLN8237 during the early stages of embryogenesis documented gradual arrest in the cleavage ability of the mouse embryo. In the AURKA-inhibited 1-cell embryos, spindle formation and anaphase onset were delayed and chromosome segregation was defective. AURKA inhibition increased apoptosis during early embryonic development. In conclusion these data suggest that AURKA is essential for the correct chromosome segregation in the first mitosis as a prerequisite for normal later development after first cleavage.
- Klíčová slova
- Apoptosis, Aurora A, MLN8237, Mouse zygote, Spindle,
- MeSH
- aurora kinasa A antagonisté a inhibitory metabolismus MeSH
- azepiny farmakologie MeSH
- časosběrné zobrazování MeSH
- fluorescenční mikroskopie MeSH
- fosforylace účinky léků MeSH
- inhibitory proteinkinas farmakologie MeSH
- konfokální mikroskopie MeSH
- kultivace embrya MeSH
- mitóza účinky léků fyziologie MeSH
- myši inbrední BALB C MeSH
- myši inbrední C57BL MeSH
- pyrimidiny farmakologie MeSH
- segregace chromozomů účinky léků fyziologie MeSH
- zvířata MeSH
- zygota účinky léků fyziologie MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- aurora kinasa A MeSH
- azepiny MeSH
- inhibitory proteinkinas MeSH
- MLN 8237 MeSH Prohlížeč
- pyrimidiny MeSH
Aurora kinase A (AURKA) is a centrosomal protein that is overexpressed in a number of human malignancies and can contribute to tumor progression. As we used this protein as a target of DNA immunization, we increased its immunogenicity by the addition of the PADRE helper epitope and decreased its potential oncogenicity by mutagenesis of the kinase domain. For in vitro analysis of induced immune responses in mice, we identified the Aurka(220-228) nonapeptide representing an H-2Kb epitope. As DNA vaccination against the Aurka self-antigen by a gene gun did not show any antitumor effect, we combined DNA immunization with anti-CD25 treatment that depletes mainly regulatory T cells. Whereas 1 anti-CD25 dose injected before DNA vaccination did not enhance the activation of Aurka-specific splenocytes, 3 doses administered on days of immunizations augmented about 10-fold immunity against Aurka. However, an opposite effect was found for antitumor immunity-only 1 anti-CD25 dose combined with DNA vaccination reduced tumor growth. Moreover, the administration of 3 doses of anti-CD25 antibody alone accelerated tumor growth. Analysis of tumor-infiltrating cells showed that 3 anti-CD25 doses not only efficiently depleted regulatory T cells but also activated helper T cells and CD3(-)CD25(+) cells. Next, we found that blockade of the PD-1 receptor initiated 1 week after the first immunization was necessary for significant inhibition of tumor growth with therapeutic DNA vaccination against Aurka combined with depletion of CD25 cells. Our results suggest that combined cancer immunotherapy should be carefully evaluated to achieve the optimal antitumor effect.
- MeSH
- aktivace lymfocytů MeSH
- antigeny CD279 antagonisté a inhibitory MeSH
- aurora kinasa A genetika imunologie metabolismus MeSH
- buňky - růstové procesy účinky léků MeSH
- CD8-pozitivní T-lymfocyty imunologie MeSH
- DNA vakcíny MeSH
- epitopy T-lymfocytární genetika imunologie metabolismus MeSH
- H-2 antigeny metabolismus MeSH
- HEK293 buňky MeSH
- imunizace MeSH
- imunoterapie * MeSH
- kombinovaná terapie MeSH
- lidé MeSH
- monoklonální protilátky terapeutické užití MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- protinádorové vakcíny imunologie MeSH
- receptor interleukinu-2 - alfa-podjednotka imunologie MeSH
- regulační T-lymfocyty imunologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny CD279 MeSH
- aurora kinasa A MeSH
- DNA vakcíny MeSH
- epitopy T-lymfocytární MeSH
- H-2 antigeny MeSH
- H-2Kb protein, mouse MeSH Prohlížeč
- monoklonální protilátky MeSH
- protinádorové vakcíny MeSH
- receptor interleukinu-2 - alfa-podjednotka MeSH
Regulation of mRNA translation by cytoplasmic polyadenylation is known to be important for oocyte maturation and further development. This process is generally controlled by phosphorylation of cytoplasmic polyadenylation element binding protein 1 (CPEB1). The aim of this study is to determine the role of Aurora kinase A in CPEB1 phosphorylation and the consequent CPEB1-dependent polyadenylation of maternal mRNAs during mammalian oocyte meiosis. For this purpose, we specifically inhibited Aurora kinase A with MLN8237 during meiotic maturation of porcine oocytes. Using poly(A)-test PCR method, we monitored the effect of Aurora kinase A inhibition on poly(A)-tail extension of long and short cyclin B1 encoding mRNAs as markers of CPEB1-dependent cytoplasmic polyadenylation. Our results show that inhibition of Aurora kinase A activity impairs neither cyclin B1 mRNA polyadenylation nor its translation and that Aurora kinase A is unlikely to be involved in CPEB1 activating phosphorylation.
- MeSH
- aurora kinasa A metabolismus MeSH
- cyklin B1 genetika MeSH
- faktory štěpení a polyadenylace mRNA chemie metabolismus MeSH
- fosforylace MeSH
- meióza * MeSH
- messenger RNA metabolismus MeSH
- oocyty enzymologie metabolismus MeSH
- polyadenylace MeSH
- Sus scrofa metabolismus MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aurora kinasa A MeSH
- cyklin B1 MeSH
- faktory štěpení a polyadenylace mRNA MeSH
- messenger RNA MeSH
