Rabbit IgG raised against whole cells of Yersinia enterocolitica O:3, O:9 and against a group of pathogenic Y. enterocolitica strains (serotypes O:3, O:5,27, O:8. and O:9) were prepared. The antibody limiting titers were within the range of 1:9.5 x 10(4)-1:7.5 x 10(5). The immunoblotting analysis of Yersinia lipopolysacchides separated by SDS-PAGE showed that IgG against the single serotype O:3 interacted with high-molar-mass LPS of O:3 whereas other antibodies were bound to low-molar-mass LPS of serotypes O:3, O:5,27, O:9 and strain Y. enterocolitica (CNCTC Y 2/68). IgG against the group of pathogenic serotypes also weakly interacted with low-molar-mass LPS of serotypes O:5, O:6,30, and O:10. The cross-reactivity of the antibodies with Y. pseudotuberculosis Ia and/or Y. rohdei b, d, e, f, i, which was observed by means of dot-blotting procedure using the whole bacterial cells as an antigen, was shown not to be caused by LPS of these bacteria. The prepared antibodies were used in the development of indirect competitive ELISA. At the optimum concentration of the immunoreactants the detection limits were within the range of 3-7 x 10(6) colony-forming units per mL.

• MeSH
• Antigens, Bacterial immunology   MeSH
• Immune Sera biosynthesis   MeSH
• Silver Staining MeSH
• Enzyme-Linked Immunosorbent Assay economics   methods   MeSH
• Endopeptidase K metabolism   MeSH
• Immunization MeSH
• Immunoblotting MeSH
• Immunoglobulin G immunology   isolation & purification   MeSH
• Immunologic Techniques MeSH
• Rabbits MeSH
• Lipopolysaccharides analysis   MeSH
• Antibodies, Bacterial analysis   immunology   MeSH
• Antibody Specificity * MeSH
• Yersinia enterocolitica chemistry   immunology   isolation & purification   MeSH
• Yersinia immunology   isolation & purification   MeSH
• Cross Reactions MeSH
• Animals MeSH
• Check Tag
• Rabbits MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Evaluation Study MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Antigens, Bacterial MeSH
• Immune Sera MeSH
• Endopeptidase K MeSH
• Immunoglobulin G MeSH
• Lipopolysaccharides MeSH
• Antibodies, Bacterial MeSH

Some patients with immunodeficiency develop clinical features of autoimmune disorders. A previously asymptomatic antibody deficiency can underlie the development of autoimmune diseases and a severe course of infection, with a risk of sepsis; such cases are known in selective IgA deficiency. On the other hand, little information is available on selective IgG subclass deficiencies. An unexpectedly severe course of Campylobacter infection in a 19-year-old woman with a previously undiagnosed complex immune disorder, including selective IgG1 immunodeficiency, Hashimoto's autoimmune thyroiditis with hypothyroidism combined with Addison's disease presumably due to autoimmune adrenalitis, autoimmunity and allergy is described. The pathophysiological mechanisms of autoimmunity in latent humoral defects are discussed.

• MeSH
• Addison Disease complications   MeSH
• Hypersensitivity complications   MeSH
• Campylobacter jejuni isolation & purification   MeSH
• IgG Deficiency complications   MeSH
• Adult MeSH
• Hashimoto Disease complications   MeSH
• Campylobacter Infections complications   microbiology   MeSH
• Humans MeSH
• Multiple Organ Failure * MeSH
• Sepsis complications   microbiology   MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Case Reports MeSH
• Research Support, Non-U.S. Gov't MeSH

High-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) were used to analyze the phospholipids and fatty acids of four Arcobacter species (becoming routinely isolated from a wide variety of food sources, especially of animal origin) to provide information for the identification within these species. Phospholipid differences were observed in the HPLC profiles. GC-MS analysis provided a complete fatty acid composition for each arcobacter that after pattern recognition analysis allows taxonomic classification of each species.

• MeSH
• Principal Component Analysis MeSH
• Arcobacter classification   metabolism   MeSH
• Phospholipids analysis   MeSH
• Classification MeSH
• Fatty Acids analysis   MeSH
• Gas Chromatography-Mass Spectrometry MeSH
• Chromatography, High Pressure Liquid MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Phospholipids MeSH
• Fatty Acids MeSH

The PCR amplicons (about 1450 bp in length) of flaA gene fragments of 11 isolates of urease-positive thermophilic Campylobacter (UPTC) isolated from the natural environment not including wild birds in Northern Ireland were demonstrated to be shorter than those of C. jejuni 81116 and six isolates of C. jejuni and C. coli (about 1700 bp) isolated in Northern Ireland and Japan. When the nucleotide lengths of the possible open reading frame (ORF) of the flaA genes were determined, those from the 11 UPTC isolates were estimated to be 1464-1503 bp, and those from the six C. jejuni and C. coli isolates and C. jejuni 81116 strain to be 1716-1728 bp. Nucleotide sequence and deduced amino acid sequence alignments of the possible ORFs demonstrated that the ORFs from the 11 UPTC isolates lack about 80 amino acid residues, mainly from the approximate residue numbers 390-470 of the large variable region in the flaA protein of the seven isolates of C. jejuni and C. coli, and do not have any internal termination codons. High amino acid sequence similarity of both amino- and carboxy-termini of the ORFs of the flaA gene was demonstrated between the 11 isolates of UPTC and the 7 isolates of C. jejuni and C. coli. The 11 UPTC isolates examined were strongly suggested to possess a shorter flaA gene without any internal termination codons.

• MeSH
• Campylobacter coli genetics   isolation & purification   MeSH
• Campylobacter jejuni genetics   isolation & purification   MeSH
• Flagellin genetics   MeSH
• Molecular Sequence Data MeSH
• Open Reading Frames MeSH
• Polymerase Chain Reaction MeSH
• Amino Acid Sequence MeSH
• Sequence Analysis, Protein MeSH
• Sequence Alignment MeSH
• Urease metabolism   MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• Japan MeSH
• Northern Ireland MeSH
• Names of Substances
• flaA protein, bacteria MeSH Browser
• Flagellin MeSH
• Urease MeSH

Model samples of Campylobacter jejuni for polymerase chain reaction (PCR) were prepared by rapid and simple procedures consisting of centrifugation, proteinase K treatment, Chelex 100 treatment, and boiling lyses. A PCR based on specific amplification of the variable sequence of 16S rRNA gene was performed using Tth DNA polymerase and the PCR products were visualized by agarose gel electrophoresis. The assay allowed the detection of 10 CFU/mL C. jejuni in the physiological saline and 100 CFU/mL in the basic Park and Sanders broth.

• MeSH
• Campylobacter coli classification   genetics   isolation & purification   MeSH
• Campylobacter jejuni classification   genetics   isolation & purification   MeSH
• Campylobacter classification   genetics   isolation & purification   MeSH
• Sodium Chloride MeSH
• Culture Media MeSH
• Humans MeSH
• Polymerase Chain Reaction methods   MeSH
• Food Microbiology * MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Evaluation Study MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Sodium Chloride MeSH
• Culture Media MeSH