Pancreatic lipase (PNLIP, EC 3.1.1.3) plays a pivotal role in the digestion of dietary lipids, a metabolic pathway directly related to obesity. One of the effective strategies in obesity treatment is the inhibition of PNLIP, which is possible to be achieved by specific phenolic compounds occurring in high abundance in some plants. In this study, a multidisciplinary approach is presented investigating the PNLIP inhibitory effect of 33 plants belonging in the Asteraceae botanical family. In the first stage of the study, a rapid and cost-efficient PNLIP assay in a 96-microwell plate format was developed and important parameters were optimized, e.g., the enzyme substrate. Upon PNLIP assay optimization, aqueous and dichloromethane Asteraceae plant extracts were tested and a cut-off inhibition level was set to further analyze only the samples with a significant inhibitory effect (inhibitory rate > 40%), using an ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) method. Specifically, a metabolomic suspect screening was performed and 69 phenolic compounds were tentatively identified, including phenolic acids, flavonoids, flavonoid-3-O-glycosides, and flavonoid-7-O-glycosides, amongst others. In the case of aqueous extracts, phytochemicals known for inducing PNLIP inhibitory effect, e.g., compounds containing galloyl molecules or caffeoylquinic acids, were monitored in Chrysanthemum morifolium, Grindella camporum and Hieracium pilosella extracts. All in all, the presented approach combines in vitro bioactivity measurements to high-end metabolomics to identify phenolic compounds with potential medicinal and/or dietary applications.

• Klíčová slova
• bioprospecting, enzyme assay, in vitro testing, metabolomics, obesity, phytochemicals, polyphenols, suspect screening, ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry,
• MeSH
• Asteraceae * chemie   MeSH
• chromatografie kapalinová MeSH
• fenoly analýza   MeSH
• flavonoidy chemie   MeSH
• fytonutrienty analýza   MeSH
• glykosidy MeSH
• hmotnostní spektrometrie MeSH
• lipasa MeSH
• lipidy MeSH
• methylenchlorid MeSH
• obezita MeSH
• rostlinné extrakty chemie   farmakologie   MeSH
• vysokoúčinná kapalinová chromatografie metody   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• fenoly MeSH
• flavonoidy MeSH
• fytonutrienty MeSH
• glykosidy MeSH
• lipasa MeSH
• lipidy MeSH
• methylenchlorid MeSH
• rostlinné extrakty MeSH

Pesticides have been extensively used in agriculture to protect crops and enhance their yields, indicating the need to monitor for their toxic residues in foodstuff. To achieve that, chromatographic methods coupled to mass spectrometry is the common analytical approach, combining low limits of detection, wide linear ranges, and high accuracy. However, these methods are also quite expensive, time-consuming, and require highly skilled personnel, indicating the need to seek for alternatives providing simple, low-cost, rapid, and on-site results. In this study, we critically review the available screening methods for pesticide residues on the basis of optical detection during the period 2016-2020. Optical biosensors are commonly miniaturized analytical platforms introducing the point-of-care (POC) era in the field. Various optical detection principles have been utilized, namely, colorimetry, fluorescence (FL), surface plasmon resonance (SPR), and surface enhanced Raman spectroscopy (SERS). Nanomaterials can significantly enhance optical detection performance and handheld platforms, for example, handheld SERS devices can revolutionize testing. The hyphenation of optical assays to smartphones is also underlined as it enables unprecedented features such as one-click results using smartphone apps or online result communication. All in all, despite being in an early stage facing several challenges, i.e., long sample preparation protocols or interphone variation results, such POC diagnostics pave a new road into the food safety field in which analysis cost will be reduced and a more intensive testing will be achieved.

• Klíčová slova
• bioassays, biosensors, food, optical detection, pesticide residues, point-of-care diagnostics, screening methods, smartphones,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

Biosensors are analytical devices combining a physical sensor with a part of biological origin providing sensitivity and selectivity toward analyte. Biological warfare agents are infectious microorganisms or toxins with the capability to harm or kill humans. They can be produced and spread by a military or misused by a terrorist group. For example, Bacillus anthracis, Francisella tularensis, Brucella sp., Yersinia pestis, staphylococcal enterotoxin B, botulinum toxin and orthopoxviruses are typical biological warfare agents. Biosensors for biological warfare agents serve as simple but reliable analytical tools for the both field and laboratory assay. There are examples of commercially available biosensors, but research and development of new types continue and their application in praxis can be expected in the future. This review summarizes the facts and role of biosensors in the biological warfare agents' assay, and shows current commercially available devices and trends in research of the news. Survey of actual literature is provided.

• Klíčová slova
• Bacillus anthracis, anthrax, bioassay, biological warfare agent, biological weapon, biosensor, colorimetry, electrochemistry, hand held assay, hemorrhagic fever, tularemia,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH