The prevalence of celiac disease (CD) was determined in healthy blood donors and in high-risk groups of adults (a total of 1835 adults--randomly selected 1312 healthy blood donors, 102 patients with primary osteoporosis, 58 patients with autoimmune diseases and 365 infertile women). It was calculated on the basis of a two-step serologic screening method--in the first step IgA and IgG antigliadin antibodies (AGA) and IgA anti-gamma-glutamyltransferase ('transglutaminase') antibodies (ATG) were estimated, in the second step sera positive for IgA AGA and/or IgA ATG were examined for antiendomysial IgA (AEA) antibodies. Immunoenzymic assay (ELISA) was used for determining of AGA and ATG antibodies; immunofluorescence method, performed on human umbilical cord tissue, was used for assaying of AEA antibodies. Total serum IgA level in only IgG AGA positive subjects was measured by routine turbidimetric method. 0.45% of healthy blood donors, 0.98% of osteoporotic patients, 2.7% of patients suffering from autoimmune disease and 1.13% of women with infertility considered as immunologically mediated were found to be positive in both steps of serologic screening (AGA and/or ATG and antiendomysium positive). The presumed high prevalence of seropositivity for CD in apparently healthy Czech adult population was confirmed. In the high-risk groups, the prevalence of seropositivity for CD was approximately 2-4 times higher than in healthy blood donors. The real prevalence of CD in the tested groups, however, can be estimated after performing small intestinal biopsy in the seropositive patients.

• MeSH
• autoimunitní nemoci komplikace   MeSH
• celiakie komplikace   diagnóza   epidemiologie   imunologie   MeSH
• dospělí MeSH
• ELISA MeSH
• gama-glutamyltransferasa imunologie   MeSH
• gliadin imunologie   MeSH
• imunoglobulin A krev   MeSH
• imunoglobulin G krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• osteoporóza komplikace   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• séroepidemiologické studie MeSH
• ženská infertilita komplikace   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH
• Názvy látek
• gama-glutamyltransferasa MeSH
• gliadin MeSH
• imunoglobulin A MeSH
• imunoglobulin G MeSH

The aim of our work was to investigate the in vitro reactivity of gliadin peptides of natural and synthetic origin with various cell lines. We have found that all tested cell lines of human, mouse and rat origin were agglutinated by enzymically digested gliadin (peptic-tryptic- and peptic-tryptic pancreatic digest of alpha-gliadin) in a concentration dependent manner. In order to test the specificity of binding, inhibition studies were performed using a panel of sugars as well as natural and synthetic peptides derived from gliadin. We have found that among twelve tested sugars only fetuin and phosphomannan were able to inhibit the agglutination of K562 cells with peptic-tryptic- but not with peptic-tryptic pancreatic digest of alpha-gliadin. The lack of inhibition by gliadin peptides and most of the saccharides suggests that agglutinating activity of gliadin is the result of a nonspecific binding of gliadin to the cell membrane.

• MeSH
• adenokarcinom patologie   MeSH
• akutní bazofilní leukemie patologie   MeSH
• alfa-fetoproteiny farmakologie   MeSH
• buněčná adheze MeSH
• Burkittův lymfom patologie   MeSH
• chronická myeloidní leukemie patologie   MeSH
• gliadin metabolismus   MeSH
• glykoproteiny membrány trombocytů * MeSH
• kmenové buňky embryonálního karcinomu MeSH
• krysa rodu Rattus MeSH
• L buňky (buněčná linie) MeSH
• lektiny MeSH
• lidé MeSH
• lymfocyty metabolismus   MeSH
• lymfom patologie   MeSH
• makrofágy metabolismus   MeSH
• mannany farmakologie   MeSH
• molekulární sekvence - údaje MeSH
• myši MeSH
• nádorové buňky kultivované MeSH
• nádorové kmenové buňky metabolismus   MeSH
• nádory maxily patologie   MeSH
• nádory tračníku patologie   MeSH
• peptidy farmakologie   MeSH
• receptory buněčného povrchu metabolismus   MeSH
• sacharidy farmakologie   MeSH
• sekvence aminokyselin MeSH
• teratokarcinom patologie   MeSH
• trombocytový glykoproteinový komplex Ib-IX * MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• alfa-fetoproteiny MeSH
• gliadin MeSH
• glycoprotein receptor GPIb-IX MeSH Prohlížeč
• glykoproteiny membrány trombocytů * MeSH
• lektiny MeSH
• mannany MeSH
• peptidy MeSH
• phosphomannan MeSH Prohlížeč
• receptory buněčného povrchu MeSH
• sacharidy MeSH
• trombocytový glykoproteinový komplex Ib-IX * MeSH

Monoclonal, hyperimmune rabbit and human serum anti-gliadin antibodies were analyzed by ELISA and immunoblotting techniques. In Western blotting the difference in reactivity between monoclonal and human antibodies was quantitative rather than qualitative. Rabbit antisera differed in reactivity according to the protein used for immunization. The rabbits immunized by the peptic-tryptic pancreatic digest of gliadin reacted similarly to the patients. In ELISA, significantly higher reactivity with crude, A-, glyc-gli, alpha-, beta- and omega-gliadins was found in the patients' sera than in controls.

• MeSH
• antisérum imunologie   MeSH
• ELISA MeSH
• gliadin imunologie   MeSH
• imunizace metody   MeSH
• králíci imunologie   MeSH
• lidé MeSH
• monoklonální protilátky imunologie   izolace a purifikace   MeSH
• myši imunologie   MeSH
• western blotting MeSH
• zvířata MeSH
• Check Tag
• králíci imunologie   MeSH
• lidé MeSH
• myši imunologie   MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antisérum MeSH
• gliadin MeSH
• monoklonální protilátky MeSH

To analyze the possible involvement of natural killer (NK) cell activity in the pathogenetic mechanism of coeliac disease (CD) we measured the spontaneous cytotoxic cell activity of peripheral blood mononuclear cells (PMNC) from patients with CD and from healthy donors. No significant differences were found between the NK cell activity of PMNC from healthy donors and from patients with CD using a standard 51 Cr release assay. However, a 30-min treatment of PMNC with gliadin inhibited NK cell activity in patients with CD. On the other hand, a 1-d incubation with gliadin induced cytotoxic cell activity of PMNC against the NK-resistant target cells such as the epithelial HT-29 and the lymphoblastoid RAJI cell lines, suggesting that activation of PMNC by cultivation with gliadin can occur.

• MeSH
• buněčná cytotoxicita závislá na protilátkách účinky léků   MeSH
• buňky NK účinky léků   imunologie   MeSH
• celiakie imunologie   MeSH
• cytotoxicita imunologická účinky léků   MeSH
• dítě MeSH
• dospělí MeSH
• gliadin farmakologie   MeSH
• kultivované buňky MeSH
• lidé MeSH
• lymfocyty účinky léků   MeSH
• nádorové buňky kultivované MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• gliadin MeSH