Sialic acids are negatively charged carbohydrates that are components of saccharide chains covalently linked to macromolecules. Sialylated glycoproteins are important for most biological processes, including reproduction, where they are associated with spermatogenesis, sperm motility, immune responses, and fertilization. Changes in the glycoprotein profile or sialylation in glycoproteins are likely to affect the quality of ejaculate. The aim of this study was to determine differences in the degree of sialylation between normozoospermic ejaculates and ejaculates with a pathological spermiogram using two lectins, Sambucus nigra (SNA) and Maackia amurensis (MAL II/MAA) recognizing α-2,6 or α-2,3 linkage of Sia to galactosyl residues. Our results show a close relationship between seminal plasma (SP) sialoproteins and the presence of anti-sperm antibodies in the ejaculate, apoptotic spermatozoa, and ejaculate quality. Using mass spectrometry, we identified SP sialoproteins such as, semenogelins, glycodelin, prolactin-inducible protein, lactotransferrin, and clusterin that are associated with spermatozoa and contribute to the modulation of the immune response and sperm apoptosis. Our findings suggest a correlation between the degree of SP glycoprotein sialylation and the existence of possible pathological states of spermatozoa and reproductive organs. Glycoproteins sialylation represents a potential parameter reflecting the overall quality of ejaculate and could potentially be utilised in diagnostics.
- Klíčová slova
- Anti-sperm antibodies, Apoptosis, Ejaculate quality, Glycoprotein, Human,
- MeSH
- analýza spermatu metody MeSH
- apoptóza MeSH
- ejakulace MeSH
- glykodelin metabolismus MeSH
- glykoproteiny metabolismus MeSH
- klusterin metabolismus MeSH
- kyseliny sialové metabolismus MeSH
- laktoferrin metabolismus MeSH
- lektiny metabolismus chemie MeSH
- lidé MeSH
- motilita spermií MeSH
- proteiny semenné plazmy metabolismus MeSH
- sekreční proteiny semenných váčků metabolismus MeSH
- sperma * metabolismus chemie MeSH
- spermie * metabolismus MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- glykodelin MeSH
- glykoproteiny MeSH
- klusterin MeSH
- kyseliny sialové MeSH
- laktoferrin MeSH
- lektiny MeSH
- PAEP protein, human MeSH Prohlížeč
- proteiny semenné plazmy MeSH
- sekreční proteiny semenných váčků MeSH
- seminal vesicle-specific antigen MeSH Prohlížeč
This study involved creating oligomeric conjugates of 3-hydroxy fatty acids and diclofenac, named Dic-oligo(3HAs). Advanced NMR techniques confirmed no free diclofenac in the mix. We tested diclofenac release under conditions resembling healthy and chronic wound skin. These oligomers were used to make P(3HO) blends, forming patches for drug delivery. Their preparation used the solvent casting/porogen leaching (SCPL) method. The patches' properties like porosity, roughness, and wettability were thoroughly analysed. Antimicrobial assays showed that Dic-oligo(3HAs) exhibited antimicrobial activity against reference (S. aureus, S. epidermis, S. faecalis) and clinical (Staphylococcus spp.) strains. Human keratinocytes (HaCaT) cell line tests, as per ISO 10993-5, showed no toxicity. A clear link between material roughness and HaCaT cell adhesion was found. Deep cell infiltration was verified using DAPI and phalloidin staining, observed under confocal microscopy. SEM also confirmed HaCaT cell growth on these scaffolds. The strong adhesion and proliferation of HaCaT cells on these materials indicate their potential as wound dressing layers. Additionally, the successful diclofenac release tests point to their applicability in treating both normal and chronic wounds.
- Klíčová slova
- Artificial skin substitute, bioresorbable, porous layer of dressing material, Poly(3-hydroxyoctanoate), Polyhydroxyalkanoates, Targeted delivery of diclofenac,
- MeSH
- biokompatibilní materiály chemie farmakologie MeSH
- buněčné linie keratinocytů HaCaT MeSH
- buněčné linie MeSH
- chemické jevy MeSH
- diklofenak * farmakologie chemie MeSH
- hojení ran účinky léků MeSH
- keratinocyty účinky léků cytologie MeSH
- kůže * účinky léků MeSH
- lidé MeSH
- polymery chemie MeSH
- poréznost MeSH
- proliferace buněk účinky léků MeSH
- regenerace účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Tau protein is an intrinsically disordered protein that plays a key role in Alzheimer's disease (AD). In brains of AD patients, Tau occurs abnormally phosphorylated and aggregated in neurofibrillary tangles (NFTs). Together with Tau, 14-3-3 proteins - abundant cytosolic dimeric proteins - were found colocalized in the NFTs. However, so far, the molecular mechanism of the process leading to pathological changes in Tau structure as well as the direct involvement of 14-3-3 proteins are not well understood. Here, we aimed to reveal the effects of phosphorylation by protein kinase A (PKA) on Tau structural preferences and provide better insight into the interaction between Tau and 14-3-3 proteins. We also addressed the impact of monomerization-inducing phosphorylation of 14-3-3 at S58 on the binding to Tau protein. Using multidimensional nuclear magnetic resonance spectroscopy (NMR), chemical cross-linking analyzed by mass spectrometry (MS) and PAGE, we unveiled differences in their binding affinity, stoichiometry, and interfaces with single-residue resolution. We revealed that the interaction between 14-3-3 and Tau proteins is mediated not only via the 14-3-3 amphipathic binding grooves, but also via less specific interactions with 14-3-3 protein surface and, in the case of monomeric 14-3-3, also partially via the exposed dimeric interface. In addition, the hyperphosphorylation of Tau changes its affinity to 14-3-3 proteins. In conclusion, we propose quite complex interaction mode between the Tau and 14-3-3 proteins.
- Klíčová slova
- 14-3-3ζ protein, Cross-linking, Interaction, NMR, Phosphorylation, Tau protein,
- MeSH
- Alzheimerova nemoc metabolismus MeSH
- fosforylace MeSH
- lidé MeSH
- molekulární modely MeSH
- multimerizace proteinu MeSH
- proteinkinasy závislé na cyklickém AMP metabolismus MeSH
- proteiny 14-3-3 * metabolismus chemie MeSH
- proteiny tau * metabolismus chemie MeSH
- vazba proteinů * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- proteinkinasy závislé na cyklickém AMP MeSH
- proteiny 14-3-3 * MeSH
- proteiny tau * MeSH
Thin composite films comprising two primary representatives of conducting polymers, poly(3, 4-ethylenedioxythiophene) (PEDOT) and polypyrrole (PPy), with eco-friendly cellulose nanocrystals (CNC) were prepared through electrochemical polymerization. The combination of CNC and PEDOT (or PPy) results in the formation of films with highly different surface topography and thickness. Intriguingly, different surface conductivity of PEDOT and PPy was revealed by atomic force microscopy albeit that the electrochemical properties were rather similar. The biological properties of the composites in contact with prospective human induced pluripotent stem cells (hiPSC) and cardiomyocytes derived from hiPSC demonstrated good cytocompatibility of both composites and their potential in engineering of electro-sensitive tissues. The as-prepared conducting, eco-friendly and cytocompatible composites are thus promising candidates for biomedical applications where stimuli-responsivity is a crucial cell-instructive property.
- Klíčová slova
- Cellulose nanocrystals, Human induced pluripotent stem cells, Poly(3,4-ethylenedioxythiophene), Polypyrrole,
- MeSH
- celulosa chemie MeSH
- indukované pluripotentní kmenové buňky * MeSH
- lidé MeSH
- nanočástice * MeSH
- polymery chemie MeSH
- prospektivní studie MeSH
- pyrroly chemie MeSH
- tkáňové inženýrství MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- celulosa MeSH
- polymery MeSH
- pyrroly MeSH
The interaction between fluorescently labeled hyaluronan and cationic surfactants was studied using Fluorescence Correlation Spectroscopy. The hyaluronan was selected at two different molecular weights - specifically, 274 kDa and 710 kDa. Cetyltrimethylammonium bromide and Septonex® were chosen as cationic surfactants to interact with the negatively charged biopolymer. The study focused on changes in the diffusive behavior of a biopolymer that interacts with surfactant molecules in an aqueous environment. Various methods were applied to evaluate the obtained data, these including, among others, the Maximum Entropy Method, which provides the distributional dependences of diffusion coefficients. Without the surfactant, the studied biopolymers showed diffusion behavior comparable to that found in previously published studies. In the presence of surfactants, more intense interaction was observed between Cetyltrimethylammonium bromide and Septonex®. Comparing the molecular weights, the retention of intermolecular aggregates after the precipitation region for the lower weight and the disintegration of these aggregates for the higher weight were observed; moreover, they showed diffusion behavior comparable to the samples without the presence of the surfactant.
- Klíčová slova
- Cetyltrimethylammonium bromide, Fluorescence correlation spectroscopy, Hyaluronan, Maximum entropy method, Septonex®,
- MeSH
- biopolymery MeSH
- cetrimonium MeSH
- fluorescenční spektrometrie MeSH
- kvartérní amoniové sloučeniny * MeSH
- kyselina hyaluronová * chemie MeSH
- povrchově aktivní látky * chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- biopolymery MeSH
- cetrimonium MeSH
- kvartérní amoniové sloučeniny * MeSH
- kyselina hyaluronová * MeSH
- povrchově aktivní látky * MeSH
- Septonex MeSH Prohlížeč
G-quadruplexes (G4s) are non-canonical nucleic acid structures that fold through complex processes. Characterization of the G4 folding landscape may help to elucidate biological roles of G4s but is challenging both experimentally and computationally. Here, we achieved complete folding of a three-quartet parallel DNA G4 with (GGGA)3GGG sequence using all-atom explicit-solvent enhanced-sampling molecular dynamics (MD) simulations. The simulations suggested early formation of guanine stacks in the G-tracts, which behave as semi-rigid blocks in the folding process. The folding continues via the formation of a collapsed compact coil-like ensemble. Structuring of the G4 from the coil then proceeds via various cross-like, hairpin, slip-stranded and two-quartet ensembles and can bypass the G-triplex structure. Folding of the parallel G4 does not appear to involve any salient intermediates and is a multi-pathway process. We also carried out an extended set of simulations of parallel G-hairpins. While parallel G-hairpins are extremely unstable when isolated, they are more stable inside the coil structure. On the methodology side, we show that the AMBER DNA force field predicts the folded G4 to be less stable than the unfolded ensemble, uncovering substantial force-field issues. Overall, we provide unique atomistic insights into the folding landscape of parallel-stranded G4 but also reveal limitations of current state-of-the-art MD techniques.
- Klíčová slova
- Folding, G-Quadruplex, Molecular dynamics,
- MeSH
- DNA chemie MeSH
- G-kvadruplexy * MeSH
- konformace nukleové kyseliny MeSH
- nukleové kyseliny * MeSH
- simulace molekulární dynamiky MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA MeSH
- nukleové kyseliny * MeSH
This work reports the synthesis of poly (itaconic acid) by thermal polymerization mediated by 2,2'-Azobis(2-methylpropionamidine) dihydrochloride. Furthermore, physical hydrogels were prepared by using high molecular weight poly (itaconic acid) characterized by low dispersity and laponite RD. The hydrogels presented porous 3D network structures, with a high-water penetration of almost 2000 g/g of swelling ratio, which can allow the adsorption sites of both poly (itaconic acid) and laponite RD to be easily exposed and facilitate the adsorption of dyes. The water adsorption followed Schott's pseudo-second-order model. The mechanism of the adsorption process was investigated using 1H and 31P NMR. The hydrogel is able to fast adsorb by a combination of electrostatic interactions and hydrogen bonding by the synergic effect of the clay and poly (itaconic acid). Moreover, the prepared aerogels exhibited a fast removal of Basic Fuchsin, with an adsorption capacity of 67.56 mg/g and a high removal efficiency (~99 %). The adsorption followed the pseudo-second-order kinetic model and Langmuir isotherm model. Furthermore, the thermodynamic parameters showed that the BF process of adsorption was spontaneous and feasible, endothermic, and followed physisorption. These results indicated that the PIA/laponite-based aerogel can be considered a promising adsorbent material in textile wastewater treatment.
- Klíčová slova
- Aggregation, Cationic dye, Composites, Metachromasy, Polyitaconic acid, Water pollution,
- MeSH
- adsorpce MeSH
- barvicí látky * chemie MeSH
- chemické látky znečišťující vodu * chemie MeSH
- hydrogely chemie MeSH
- kinetika MeSH
- silikáty * MeSH
- sukcináty * MeSH
- voda MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- barvicí látky * MeSH
- chemické látky znečišťující vodu * MeSH
- hydrogely MeSH
- itaconic acid MeSH Prohlížeč
- laponite MeSH Prohlížeč
- silikáty * MeSH
- sukcináty * MeSH
- voda MeSH
This work describes the preparation of a novel biopolymer hydrogel based on acid whey, cellulose derivatives and polyvinyl alcohol (PVA). The hydrogel was prepared and characterized with the aim of producing an environmentally-friendly soil amendment to increase water retention capacity of the soil. The findings showed considerable swelling properties of the hydrogels depending on the PVA content and crosslinking density. The samples with PVA in a concentration 2.5 % and 5 % were more rigid, the gel fraction increased with a subsequently decrease in their swelling capacity. The hydrogels crosslinked with 15 % of citric acid demonstrated a constant swelling ratio (SR) of around 500 % within 10 swelling/drying cycles. The hydrogels crosslinked with 10 % citric acid and supplemented with 1 % of PVA showed SR of 1000-1400 % caused by less crosslinked polymer network and increased pore volume for water uptake. It was found that hydrogel with a higher gel fraction had a stable structure. Supplementing PVA at 5 % extended the period of decomposition of the hydrogel material by almost 60 % in the soil environment and soil humidity was maintained for longer. Applying 2 % of the hydrogel 5PVA to soil increased the water retention capacity by 19 %.
- Klíčová slova
- Acid whey, Polyvinyl alcohol, Renewable hydrogel, Sustainability, Water retention,
- MeSH
- hydrogely * chemie MeSH
- kyselina citronová MeSH
- polysacharidy MeSH
- polyvinylalkohol * chemie MeSH
- půda MeSH
- syrovátka MeSH
- syrovátkové proteiny MeSH
- voda MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- hydrogely * MeSH
- kyselina citronová MeSH
- polysacharidy MeSH
- polyvinylalkohol * MeSH
- půda MeSH
- syrovátkové proteiny MeSH
- voda MeSH
Using an active targeting approach of chemotherapeutics-loaded nanocarriers (NCs) with monoclonal antibodies is a potential strategy to improve the specificity of the delivery systems and reduce adverse reactions of chemotherapeutic drugs. Specific targeting of the human epidermal growth factor receptor-2 (HER-2), expressed excessively in HER-2-positive breast cancer cells, can be achieved by conjugating NCs with an anti-HER-2 monoclonal antibody. We constructed trastuzumab-conjugated chitosan iodoacetamide-coated NCs containing doxorubicin (Tras-Dox-CHI-IA-NCs) as a tumor-targeted drug delivery system, during the study. Chitosan-iodoacetamide (CHI-IA) was synthesized and utilized to prepare trastuzumab-conjugated NCs (Tras-NCs). The morphology, physicochemical properties, drug loading, drug release, and biological activities of the NCs were elucidated. The Tras-NCs were spherical, with a particle size of approximately 76 nm, and had a positive zeta potential; after incorporating the drug, the size of the Tras-NC increased. A prolonged, 24-h drug release from the NCs was achieved. The Tras-NCs exhibited high cellular accumulation and significantly higher antitumor activity against HER-2-positive breast cancer cells than the unconjugated NCs and the drug solution. Therefore, Tras-Dox-CHI-IA-NCs could be a promising nanocarrier for HER-2-positive breast cancer.
- Klíčová slova
- Chitosan-iodoacetamide, Doxorubicin, Nanocarriers, Trastuzumab, Tumor targeting,
- MeSH
- chitosan * chemie MeSH
- doxorubicin chemie MeSH
- jodacetamid MeSH
- lidé MeSH
- monoklonální protilátky chemie MeSH
- nádory prsu * farmakoterapie MeSH
- nanočástice * chemie MeSH
- nosiče léků chemie MeSH
- systémy cílené aplikace léků MeSH
- trastuzumab MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chitosan * MeSH
- doxorubicin MeSH
- jodacetamid MeSH
- monoklonální protilátky MeSH
- nosiče léků MeSH
- trastuzumab MeSH
In this study, furcellaran (FUR) obtained from Furcellaria lumbricalis was firstly employed for sulfation via various methods, including SO3-pyridine (SO3∙Py) complex in different aprotic solvents, chlorosulfonic acid and sulfuric acid with a "coupling" reagent N,N'-Dicyclohexylcarbodiimide. Structural characterization through FT-IR, GPC, XPS and elemental analyses confirmed the successful synthesis of 6-O-sulfated FUR derivates characterized by varying degrees of sulfation (DS) ranging from 0.15 to 0.91 and molecular weight (Mw) spanning from12.5 kDa to 2.7 kDa. In vitro clotting assays, partial thromboplastin time (aPTT), thrombin time (TT), and prothrombin time (PT) underscored the essential role of sulfate esters in conferring anticoagulant activity whereas FUR prepared via chlorosulfonic acid with DS of 0.91 reached 311.4 s in aPPT showing almost 4-fold higher anticoagulant activity than native FUR at the concentration 2 mg/mL. MTT test showed all tested samples decreased cell viability in a dose dependent manner while all of them are non-cytotoxic up to the concentration of 0.1 mg/mL. Furthermore, sulfated derivates deposited onto polyethylene terephthalate surface presented substantial decrease in platelet adhesion, as well as absence of the most activated platelet stages. These findings support the pivotal role of O-6 FUR sulfates in enhancing hemocompatibility and provide valuable insights for a comparative assessment of effective sulfating approaches.
- Klíčová slova
- Anticoagulant, Furcellaran, Hemocompatibility, Platelet adhesion, Seaweed polysaccharide, Sulfation,
- MeSH
- algináty * MeSH
- antikoagulancia * farmakologie MeSH
- hemokoagulace * MeSH
- kyseliny sulfonové * MeSH
- parciální tromboplastinový čas MeSH
- rostlinné gumy * MeSH
- sírany chemie MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- algináty * MeSH
- antikoagulancia * MeSH
- chlorosulfonic acid MeSH Prohlížeč
- furcellaran MeSH Prohlížeč
- kyseliny sulfonové * MeSH
- rostlinné gumy * MeSH
- sírany MeSH
