Non-small cell lung carcinoma (NSCLC) represents the majority of lung cancer cases, comprising approximately 85 % of the total. The five-year survival rate for NSCLC patients remains discouragingly low. Recently, immunotherapy has emerged as a promising approach. Nevertheless, only a minority of patients experience considerable benefits from these treatments. This highlights the critical need for effective biomarkers that can predict both patient prognosis and response to immunotherapy. CD8+ T cells play a crucial role in cancer immunotherapy. Their presence within tumours is generally indicative of a favourable prognosis and increased efficacy of immunotherapy. This study was undertaken to identify and authenticate a novel biomarker signature based on CD8+ T-cell marker genes, to prognosticate therapeutic responses in individuals afflicted with NSCLC. This in-depth study was based on a total of 1,200 samples, which included four NSCLC specimens analysed through single-cell RNA sequencing (scRNA-seq), 1,000 NSCLC samples obtained from The Cancer Genome Atlas (TCGA) and 196 NSCLC specimens collected from the GSE37745 cohort. In patients with NSCLC, those presenting a favourable risk profile demonstrated notable elevations in specific immune cells while concurrently exhibiting reductions in other types. CD8+ T cells, with their established role in inducing apoptosis in cancer cells, have emerged as crucial predictors and modulators of treatment strategies for NSCLC patients. The combination of single-cell and bulk RNA sequencing has produced a biomarker signature, emphasizing the CD8+ T cells' crucial role in NSCLC prognosis and treatment.
- Klíčová slova
- CD8+ T-cell marker genes, NSCLC, bulk RNA sequencing, immunotherapy response, prognostic signature, single-cell,
- MeSH
- CD8-pozitivní T-lymfocyty * imunologie MeSH
- imunoterapie * metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové biomarkery MeSH
- nádory plic * imunologie terapie MeSH
- nemalobuněčný karcinom plic * imunologie terapie MeSH
- prognóza MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- nádorové biomarkery MeSH
We aimed to detect the levels of T-lympho-cyte subsets and serum tumour markers in patients with non-small cell lung cancer (NSCLC) before chemotherapy, and to investigate the predictive value of their combined detection for the prognosis of NSCLC patients undergoing chemotherapy. The clinical data of 110 NSCLC patients treated with chemotherapy from January 2019 to February 2021 were analysed retrospectively. All patients were followed up for one year and divided into good prognosis group (surviving cases) and poor prognosis group (deceased cases). The predictive value of T-lymphocyte subsets combined with serum tumour markers for prognosis was analysed. The proportions of patients with tumour-node-metastasis stages III-IV, lymph node metastasis and poor differentiation were higher in the poor prognosis group than those in the good prognosis group (P < 0.05). Cox regression analysis revealed that high expression of CD4+ and CEA represented protective factors for poor prognosis of NSCLC patients undergoing chemotherapy [odds ratio (OR) < 1, P < 0.05], while high expression of CA125 was a risk factor (OR > 1, P < 0.05). All the areas under the receiver operating characteristic curves of single indicator detection (CD4+, CEA and CA125 levels) and their combined detection for prediction of the poor prognosis of NSCLC patients undergoing chemotherapy were > 0.70, which was highest in the case of combined detection. T-lymphocyte subsets and serum tumour markers are closely related to the prognosis of NSCLC patients undergoing chemotherapy, and their combined detection is of high predictive value.
- Klíčová slova
- T-lymphocyte subset, non-small cell lung cancer, prediction, prognosis, tumour marker,
- MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové biomarkery * krev MeSH
- nádory plic * farmakoterapie krev patologie MeSH
- nemalobuněčný karcinom plic * farmakoterapie krev MeSH
- prediktivní hodnota testů MeSH
- prognóza MeSH
- retrospektivní studie MeSH
- senioři MeSH
- T-lymfocyty - podskupiny * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- nádorové biomarkery * MeSH
High temperatures severely affect plant growth and development leading to major yield losses. These temperatures are expected to increase further due to global warming, with longer and more frequent heat waves. Rhamnolipids (RLs) are known to protect several plants against various pathogens. To date, how RLs act under abiotic stresses is unexplored. In this study, we aimed to investigate whether RLs could modify Arabidopsis thaliana physiology during prolonged heat stress. Measurement of leaf gas exchange and chlorophyll fluorescence showed that heat stress reduces photosynthetic rate through stomatal limitation and reduction of photosystem II yield. Our study reported decreased chlorophyll content and accumulation of soluble sugars and proline in response to heat stress. RLs were shown to have no detrimental effect on photosynthesis and carbohydrate metabolism in all conditions. These results extend the knowledge of plant responses to prolonged heat stress.
- Klíčová slova
- chlorophyll fluorescence, gas exchange, photosynthesis, soluble sugars, thermotolerance,
- MeSH
- Arabidopsis * fyziologie účinky léků metabolismus MeSH
- chlorofyl metabolismus MeSH
- fotosyntéza * účinky léků MeSH
- fotosystém II (proteinový komplex) metabolismus MeSH
- glykolipidy * farmakologie MeSH
- listy rostlin fyziologie účinky léků metabolismus MeSH
- prolin metabolismus MeSH
- reakce na tepelný šok * účinky léků MeSH
- vysoká teplota MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chlorofyl MeSH
- fotosystém II (proteinový komplex) MeSH
- glykolipidy * MeSH
- prolin MeSH
- rhamnolipid MeSH Prohlížeč
Our understanding of the vertebrate immune system is dominated by a few model organisms such as mice. This use of a few model systems is reasonable if major features of the immune systems evolve slowly and are conserved across most vertebrates, but may be problematic if there is substantial macroevolutionary change in immune responses. Here, we present a test of the macroevolutionary stability, across 15 species of jawed fishes, of the transcriptomic response to a standardized immune challenge. Intraperitoneal injection of an immune adjuvant (alum) induces a fibrosis response in nearly all jawed fishes, which in some species contributes to anti-helminth resistance. Despite this conserved phenotypic response, the underlying transcriptomic response is highly inconsistent across species. Although many gene orthogroups exhibit differential expression between saline versus alum-injected fish in at least one species, few orthogroups exhibit consistent differential expression across species. This result suggests that although the phenotypic response to alum (fibrosis) is highly conserved, the underlying gene regulatory architecture is very flexible and cannot readily be extrapolated from any one species to fishes (or vertebrates) more broadly. The vertebrate immune response is remarkably changeable over macroevolutionary time, requiring a diversity of model organisms to describe effectively.
- Klíčová slova
- evolutionary immunology, fibrosis, transcriptome,
- Publikační typ
- časopisecké články MeSH
- preprinty MeSH
The presence of pharmaceuticals in nature systems poses a threat to the environment, plants, animals, and, last but not least, human health. Their transport in soils, waters, and sediments plays important roles in the toxicity and bioavailability of pharmaceuticals. The mobility of pharmaceuticals can be affected by their interactions with organic matter and other soil and water constituents. In this study, a model agarose hydrogel enriched by humic acid as a representative of organic matter is used as a transport medium for pharmaceuticals. Sulphapyridine (as a representative of sulphonamide antibiotics) and diclofenac (as a representative of widely used non-steroidal anti-inflammatory drugs) were chosen for experiments in diffusion cells. Pharmaceuticals were passed through the hydrogel from the donor solution to the acceptor compartment and could interact with humic acids incorporated in the hydrogel. The lag time was prolonged if the hydrogel was enriched by humic acids from 134 to 390 s for sulphapyridine and from 323 to 606 s for diclofenac. Similarly, the incorporation of humic acids in the hydrogel resulted in a decrease in the determined diffusion coefficients. The decrease was stronger in the first stage of the experiment when diffusing particles could interact with vacant binding sites.
- Klíčová slova
- diclofenac, humic acid, hydrogel, sulphapyridine, transport,
- MeSH
- difuze MeSH
- diklofenak * chemie MeSH
- huminové látky * analýza MeSH
- hydrogely * chemie MeSH
- léčivé přípravky chemie MeSH
- sefarosa * chemie MeSH
- sulfapyridin chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- diklofenak * MeSH
- huminové látky * MeSH
- hydrogely * MeSH
- léčivé přípravky MeSH
- sefarosa * MeSH
- sulfapyridin MeSH
The escalating global water scarcity demands innovative solutions, one of which is hydroponic vegetable cultivation systems that increasingly use reclaimed wastewater. Nevertheless, even treated wastewater may still harbor various emerging organic contaminants, including pharmaceuticals. This study aimed to comprehensively assess the impact of pharmaceuticals, focusing on bioconcentration factors (BCFs), translocation factors (TFs), pharmaceutical persistence in aqueous environment, ecotoxicological end points, and associated environmental and health risks. Lettuce (Lactuca sativa) was cultivated hydroponically throughout its entire growth cycle, exposed to seven distinct concentration levels of contaminants ranging from 0 to 500 μg·L-1 over a 35-day period. The findings revealed a diverse range of BCFs (2.3 to 880 L·kg-1) and TFs (0.019-1.48), suggesting a high potential of pharmaceutical uptake and translocation by L. sativa. The degradation of 20 pharmaceuticals within the water-lettuce system followed first-order degradation kinetics. Substantial ecotoxicological effects on L. sativa were observed, including increased mortality, alterations in root morphology and length, and changes in biomass weight (p < 0.05). Furthermore, the estimated daily intake of pharmaceuticals through L. sativa consumption suggested considerable health risks, even if lettuce would be one of the many vegetables consumed. It is hypothetical, as the values were calculated. Moreover, this study assessed the environmental risk associated with the emergence of antimicrobial resistance (AMR) in aquatic environments, revealing a significantly high risk of AMR emergence. In conclusion, these findings emphasize the multifaceted challenges posed by pharmaceutical contamination in aquatic environments and the necessity of proactive measures to mitigate associated risks to both environmental and human health.
- Publikační typ
- časopisecké články MeSH
High-velocity stars and peculiar G objects orbit the central supermassive black hole (SMBH) Sagittarius A* (Sgr A*). Together, the G objects and high-velocity stars constitute the S cluster. In contrast with theoretical predictions, no binary system near Sgr A* has been identified. Here, we report the detection of a spectroscopic binary system in the S cluster with the masses of the components of 2.80 ± 0.50 M⊙ and 0.73 ± 0.14 M⊙, assuming an edge-on configuration. Based on periodic changes in the radial velocity, we find an orbital period of 372±3 days for the two components. The binary system is stable against the disruption by Sgr A* due to the semi-major axis of the secondary being 1.59±0.01 AU, which is well below its tidal disruption radius of approximately 42.4 AU. The system, known as D9, shows similarities to the G objects. We estimate an age for D9 of 2 . 7 - 0.3 + 1.9 × 1 0 6 yr that is comparable to the timescale of the SMBH-induced von Zeipel-Lidov-Kozai cycle period of about 106 yr, causing the system to merge in the near future. Consequently, the population of G objects may consist of pre-merger binaries and post-merger products. The detection of D9 implies that binary systems in the S cluster have the potential to reside in the vicinity of the supermassive black hole Sgr A* for approximately 106 years.
- Publikační typ
- časopisecké články MeSH
Traumatic brain injury (TBI) has long-term consequences, including neurodegenerative disease risk. Current diagnostic tools are limited in detecting subtle brain damage. This review explores emerging biomarkers for TBI, including those related to neuronal injury, inflammation, EVs, and ncRNAs, evaluating their potential to predict clinical outcomes like mortality, recovery, and cognitive impairment. It addresses challenges and opportunities for implementing biomarkers in clinical practice, aiming to improve TBI diagnosis, prognosis, and treatment.
- Klíčová slova
- biomarkers, clinical outcomes, extracellular vesicles, non-coding RNAs, traumatic brain injury,
- Publikační typ
- časopisecké články MeSH
PURPOSE: The aim of the paper is to emphasize the importance of physical activity in prisons, its link to mental health and the potential for desistance bringing the perspective of two Eastern European countries - Czechia and Hungary. The paper aims to show that sport in prison has to be seriously considered as an activity that has the potential to positively contribute to the physical and mental health of prison inmates. The aim of the paper is to show that sport in prison is a very potential rehabilitative tool. Doing sport in prison presents an opportunity for meaningful leisure and contributes to the development of good leisure habits. DESIGN/METHODOLOGY/APPROACH: The methodology integrates a literature review and legal analysis, complemented by practical experiences, which provide both theoretical and empirical understanding of the research topic; the review covers international research papers regarding sports activities in prisons and also the legal framework of the topic, both the international one and the national (Czech and Hungarian) ones providing the experience with concrete activities from the Czech and Hungarian prisons as well. FINDINGS: Sport in prison has to be seriously considered as an activity that has the potential to positively contribute to the physical and mental health of prison inmates. Sport in prison is a very potential rehabilitative tool. Doing sport in prison presents an opportunity for meaningful leisure and contributes to the development of good leisure habits. The possibility for prison inmates to do sports activities corresponds to a comprehensive approach to prison treatment and rehabilitation which works with leisure time as a pro-desistance factor. RESEARCH LIMITATIONS/IMPLICATIONS: This is not extensive research, rather it is a theoretical mapping with national (Czech and Hungarian) experience. PRACTICAL IMPLICATIONS: Physical activity in prisons should be officially recognised (politically and systematically) as an activity with significant potential to improve both the physical and mental health of inmates, serving as an effective rehabilitative tool. From a systemic perspective, allowing physical activities in prisons reflects the trend towards normalising prison life, addressing issues related to prisonisation. Engaging in physical activity can bridge the gap between prison and post-release life, helping individuals maintain and cultivate pro-social habits developed during incarceration. For that reason, it should be considered as relevant part of prison throughcare and aftercare. SOCIAL IMPLICATIONS: Improvement of physical and mental health of prison inmates, as well as their socialisation. Increasing the rehabilitation potential of the prison system. Contribution to desistance from crime through leisure-time physical activity as a pro-desistance factor. ORIGINALITY/VALUE: It is a theoretical analysis of the research topic focused on two Eastern European countries, Czechia and Hungary, including examples of national practices, which is interesting for international readers.
- Klíčová slova
- Desistance, Mental health, Physical activity, Prison, Rehabilitation, Sport,
- Publikační typ
- časopisecké články MeSH
Hot electrons (HEs) represent out-of-equilibrium carriers that are capable of facilitating reactions which are inaccessible under conventional conditions. Despite the similarity of the HE process to catalysis, optimization strategies such as orbital alignment and adsorption kinetics have not received significant attention in enhancing the HE-driven reaction yield. Here, we investigate catalytic effects in HE-driven reactions using a compositional catalyst modification (CCM) approach. Through a top-down alloying process and systematic characterization, using electrochemical, photodegradation, and ultrafast spectroscopy, we are able to disentangle chemical effects from competing electronic phenomena. Correlation between reactant energetics and the HE reaction yield demonstrates the crucial role of orbital alignment in HE catalytic efficiency. Optimization of this parameter was found to enhance HE reaction efficiency 5-fold, paving the way for tailored design of HE-based catalysts for sustainable chemistry applications. Finally, our study unveils an emergent ordering effect in photocatalytic HE processes that imparts the catalyst with an unexpected polarization dependence.
- Klíčová slova
- Bimetallic Alloy, Compositional Catalyst Modification, Gradient, Hot-Electron, Photocatalysis,
- Publikační typ
- časopisecké články MeSH
