• Klíčová slova
• ADHESIONS/therapy *, CORTISONE/therapy *, URETHRA/diseases *,
• MeSH
• adheze tkání terapie   MeSH
• kortison terapie   MeSH
• lidé MeSH
• nemoc * MeSH
• stenóza MeSH
• uretra * MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• kortison MeSH

The termination of different subsets in apparently homogeneous cell population of T lymphocytes seems to be extremely important in the investigation of allergic diseases and can be of diagnostical use. It has been proved that the dysfunction of those subsets is very important pathogenetic mechanism of atopy. The existing technique using monoclonal antibodies to cell surface markers is rather expensive and has even some disadvantages. It has been published recently that some strains of bacteria were capable to bind spontaneously to human lymphocytes. Simultaneous use of more bacterial strains permitted to identify two B-cells and four T-cells subsets. The interaction of lectins on the lymphocyte surface and polysaccharide substances of the bacterial cell wall is supposed to be the mechanism of binding. In the present study an attempt was made to find out whether there is a difference in the binding of bacteria to lymphocytes between normal subjects and atopics and whether changes occur in this phenomenon after application of immunotherapy. It has been found that this qualitatively novel way of binding cannot be used for laboratory characterization of atopic subjects. No differences were observed in T-lymphocyte subsets identified by bacterial adherence between allergic and normal subjects. Immunotherapy failed to influence the binding. No correlation has been found with the method using monoclonal antibodies. Statistical evaluation revealed considerable dispersion of results obtained.

• MeSH
• B-lymfocyty klasifikace   MeSH
• bakteriální adheze * MeSH
• časná přecitlivělost krev   diagnóza   terapie   MeSH
• desenzibilizace imunologická MeSH
• lidé MeSH
• lymfocyty klasifikace   imunologie   MeSH
• monoklonální protilátky MeSH
• T-lymfocyty klasifikace   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• monoklonální protilátky MeSH

Synechiae of the vulva occurs especially in girls in the so-called resting hormonal period with a minimal serum concentration of estrogens. If the external genitalia of the girl are not treated properly, the mucous membrane of the vaginal entrance is not regularly checked and mechanically removed, then the smegma of the opposite mucous membranes of the vulva can stick together. The place of the adhesion of epithelium in the vaginal introitus can be covered by a new epithelium and can create a firm adhesion, synechiae of the vulva, which is usually asymptomatic and is discovered incidentally during pediatric examination. Clinically significant vulvar synechiae can cause impaired bladder evacuation and recurrent lower urinary tract infections. Dissection of synechiae in the stage of conglutination or thin adhesion is performed manually on an outpatient basis, but extensive and firm adhesions must be divided surgically under general anesthesia. Local application of cream with estriol or betamethasone is also used for the therapy of synechiae of the vulva, but this treatment is more time-consuming compared to rapid manual separation and requires good compliance of the girls and their parents. The prevention of synechiae is based on the education of parents by pediatricians and gynecologists about the need for regular checks of the external genitalia and sufficient hygiene.

• Klíčová slova
• conglutination vulvae infantum, conservative therapy, conservative treatment, manual separation, synechiae of the vulva, vulvovaginitis,
• MeSH
• adheze tkání etiologie   MeSH
• dítě MeSH
• estrogeny MeSH
• lidé MeSH
• nemoci vulvy * etiologie   patologie   terapie   MeSH
• vagina MeSH
• vulva patologie   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• estrogeny MeSH

Psoriasis is a chronic inflammatory skin disease which is often manifested during childhood. The present study investigated changes in the serum levels of proinflammatory cytokines and soluble forms of adhesion molecules in children with psoriasis. The observed patient group of 26 children was treated with the Goeckerman regimen. This therapy combines dermal application of crude coal tar with ultraviolet radiation. The Psoriasis Area Severity Index decreased significantly after treatment by with the Goeckerman regimen (p < 0.001). Serum levels of the proinflammatory cytokine TNF-alpha and adhesion molecules sICAM-1, sP-selectin and sE-selectin decreased after the Goeckerman regimen. The TNF-alpha and sICAM-1 decreased significantly (p < 0.05). Our findings support the complex role of these immune parameters in the immunopathogenesis of psoriasis in children. The serum level of IL-8 increased after the Goeckerman regimen. This fact indicates that the chemokine pathway of IL-8 activity could be modulated by this treatment, most likely by polycyclic aromatic hydrocarbons.

• MeSH
• aplikace lokální MeSH
• dávka záření MeSH
• dehet uhelný aplikace a dávkování   MeSH
• dítě MeSH
• ELISA MeSH
• fototerapie metody   MeSH
• interleukin-8 krev   MeSH
• kombinovaná terapie MeSH
• lidé MeSH
• mladiství MeSH
• molekuly buněčné adheze krev   MeSH
• psoriáza krev   imunologie   terapie   MeSH
• stupeň závažnosti nemoci MeSH
• TNF-alfa krev   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• dehet uhelný MeSH
• interleukin-8 MeSH
• molekuly buněčné adheze MeSH
• TNF-alfa MeSH

BACKGROUND: The treatment of malignancies like acute myeloid leukemia (AML) is often complicated by the heterogeneity of the disease and the mechanisms of the disease progression. This heterogeneity is often not reflected in standard treatment approaches which provide predictable outcomes in the majority of patients but fail in individual cases even with high-dose multi-agent chemotherapy regimens and allogeneic stem cell transplantation. Further, the unselective effect of chemotherapy causes high treatment-related toxicity and accelerates the risk of infection during prolonged pancytopenia, preventing further dose escalation. Despite rapid progress in therapeutic strategies, the fatality of high-grade malignancies remains enormous. OBJECTIVES: Adhesive interactions trigger signal transduction pathway activation and this prevents the apoptosis of both normal and malignant cells. A correlation between expression of defined adhesion molecules and patient outcome has been found for several malignant diseases including AML. We aim to describe how disruption of these signalling pathways can overcome the high resistance to treatment and increase the selectivity of targeting malignant cells. This could effectively reduce the overall treatment-related toxicity and improve the general outcome. CONCLUSIONS: Adhesion molecules facilitate growth of malignant diseases. This review provides a deeper insight into these processes. Modulation of adhesion molecules-mediated interactions is an innovative and feasible approach in treatment of AML and many other malignancies. Due to expected low toxicity it is an acceptable addition to standard chemotherapeutical regimens for all age groups of patients. This approach could improve the overall treatment outcome in the future.

• MeSH
• akutní myeloidní leukemie metabolismus   terapie   MeSH
• kombinovaná terapie MeSH
• lidé MeSH
• molekuly buněčné adheze * MeSH
• prognóza MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• systematický přehled MeSH
• Názvy látek
• molekuly buněčné adheze * MeSH

AIM: Early diagnosis of the progressive tumor disease and control of the effect of therapy in colorectal carcinoma are most frequently performed by monitoring CEA or CA 19-9 tumor markers. Their clinical application is, however, limited. The aim of our study was to demonstrate the contribution of adhesive molecule assessment to the early diagnosis of progression. We also wanted to find out if changes in the levels of cellular adhesion parameters correlate with the effect of antitumor therapy. MATERIALS AND METHODS: Intercellular cell adhesive molecule-1 (ICAM-1) and Vascular cell adhesive molecule-1 (VCAM-1) were assessed using the ELISA method, and the results were correlated with CEA and CA 19-9 tumor markers. Three hundred and sixty-four patients with colorectal carcinoma in Dukes' stages B-D were monitored. The results were processed with the SAS 6.2. statistical program and Statistica. RESULTS: In 92 patients with first clinical progression (occurrence of distant metastases irrespective of localization), significantly increased ICAM-1 and VCAM-1 values were demonstrated. In ROC evaluation of curves, we also demonstrated high sensitivity of adhesive molecules against both the control healthy group (n =89) and the no evidence of disease group (NED) (n=183). Adhesive molecule levels were closely connected with the type and course of therapy and are presented in the form of case reports. CONCLUSION: Soluble adhesive molecules are a prospective parameter both for the early diagnosis of progression and for control of the effect of therapy. There is a need for a large-scale study, preferably multicentric, which would verify the suitability of introducing cellular adhesion parameter assessment into routine practice.

• MeSH
• buněčná adheze * MeSH
• kolorektální nádory diagnóza   patologie   terapie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• molekuly buněčné adheze krev   MeSH
• nádorové biomarkery krev   MeSH
• pilotní projekty MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• molekuly buněčné adheze MeSH
• nádorové biomarkery MeSH

Psoriasis is one of the most frequent inflammatory skin diseases in which abnormal individual immune reactivity plays an important role. The aim of the present study was to describe selected immunological changes, concerning pro-inflammatory cytokines (TNF-alpha, IL-8) and adhesion molecules (sE-selectin, sP-selectin, sICAM-1), in 56 patients cured by Goeckerman's therapy (GT). GT includes dermal application of crude coal tar (containing polycyclic aromatic hydrocarbons) and exposure to UV radiation. When compared with the control group (healthy blood donors), the patients before GT had significantly increased serum levels of sE-selectin (p<0.001), sP-selectin (p<0.001), sICAM-1 (p<0.001) and IL-8 (p<0.001). Significantly decreased serum levels of sE-selectin (p<0.05) and significantly increased serum levels of IL-8 (p<0.05) were found after GT therapy. Serum levels of sICAM significantly correlated with the disease activity and with serum levels of sE-selectin. The level of PASI score (Psoriasis Area and Severity Index) significantly decreased after GT (p<0.001) and confirms the high efficiency GT. These findings confirmed that pro-inflammatory chemokine (IL-8) and adhesion molecules (sE-selectin, sP-selectin, sICAM-1) play an important role in the development and regulation of inflammation in psoriasis. Determination of sE-selectin and sICAM seems to be a promising marker of psoriasis's activity. Chemokine pathway (IL-8) and TNF-alpha activity seem to be modulated by Goeckerman's therapy (polycyclic aromatic hydrocarbons).

• MeSH
• biologické markery krev   MeSH
• časové faktory MeSH
• cytokiny krev   MeSH
• dehet uhelný terapeutické užití   MeSH
• dospělí MeSH
• E-selektin krev   MeSH
• interleukin-8 krev   MeSH
• keratolytika terapeutické užití   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mezibuněčná adhezivní molekula-1 krev   MeSH
• mladiství MeSH
• molekuly buněčné adheze krev   MeSH
• P-selektin krev   MeSH
• psoriáza farmakoterapie   imunologie   radioterapie   MeSH
• senioři MeSH
• stupeň závažnosti nemoci MeSH
• terapie ultrafialovými paprsky * MeSH
• TNF-alfa krev   MeSH
• výsledek terapie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky kontrolované MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické markery MeSH
• cytokiny MeSH
• dehet uhelný MeSH
• E-selektin MeSH
• interleukin-8 MeSH
• keratolytika MeSH
• mezibuněčná adhezivní molekula-1 MeSH
• molekuly buněčné adheze MeSH
• P-selektin MeSH
• TNF-alfa MeSH

Mast cells and basophils (MCs/Bs) play a crucial role in type I allergy, as well as in innate and adaptive immune responses. These cells mediate their actions through soluble mediators, some of which are targeted therapeutically by, for example, H1- and H2-antihistamines or cysteinyl leukotriene receptor antagonists. Recently, considerable progress has been made in developing new drugs that target additional MC/B mediators or receptors, such as serine proteinases, histamine 4-receptor, 5-lipoxygenase-activating protein, 15-lipoxygenase-1, prostaglandin D2, and proinflammatory cytokines. Mediator production can be abrogated by the use of inhibitors directed against key intracellular enzymes, some of which have been used in clinical trials (eg, inhibitors of spleen tyrosine kinase, phosphatidylinositol 3-kinase, Bruton tyrosine kinase, and the protein tyrosine kinase KIT). Reduced MC/B function can also be achieved by enhancing Src homology 2 domain-containing inositol 5' phosphatase 1 activity or by blocking sphingosine-1-phosphate. Therapeutic interventions in mast cell-associated diseases potentially include drugs that either block ion channels and adhesion molecules or antagonize antiapoptotic effects on B-cell lymphoma 2 family members. MCs/Bs express high-affinity IgE receptors, and blocking their interactions with IgE has been a prime goal in antiallergic therapy. Surface-activating receptors, such as CD48 and thymic stromal lymphopoietin receptors, as well as inhibitory receptors, such as CD300a, FcγRIIb, and endocannabinoid receptors, hold promising therapeutic possibilities based on preclinical studies. The inhibition of activating receptors might help prevent allergic reactions from developing, although most of the candidate drugs are not sufficiently cell specific. In this review recent advances in the development of novel therapeutics toward different molecules of MCs/Bs are presented.

• Klíčová slova
• Mast cell, basophil, drug, mediator, receptor, signaling protein, survival protein, therapy,
• MeSH
• alergie imunologie   terapie   MeSH
• antialergika farmakologie   terapeutické užití   MeSH
• apoptóza účinky léků   MeSH
• bazofily imunologie   MeSH
• cílená molekulární terapie MeSH
• degranulace buněk účinky léků   MeSH
• imunoterapie metody   trendy   MeSH
• iontové kanály antagonisté a inhibitory   MeSH
• lidé MeSH
• mastocyty imunologie   MeSH
• molekuly buněčné adheze antagonisté a inhibitory   MeSH
• receptory IgE antagonisté a inhibitory   MeSH
• receptory kanabinoidní metabolismus   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• antialergika MeSH
• iontové kanály MeSH
• molekuly buněčné adheze MeSH
• receptory IgE MeSH
• receptory kanabinoidní MeSH

BACKGROUND: There is no study focusing on changes in coronary atherosclerosis during dual lipid-lowering therapy with statin and ezetimibe. METHODS AND RESULTS: Eighty-nine patients with stable angina randomized in a 1:1 ratio to Group A (aggressive therapy: atorvastatin 80mg, ezetimibe 10mg) and Group S (standard therapy) were analyzed. Treatment period was 12 months. Coronary arteries were examined by intravascular ultrasound and virtual histology. We found a decrease in the percent atheroma volume (PAV) (-0.4%) in Group A compared with an increase (+1.4%) in Group S (P=0.014) and this was accompanied by an increased frequency of combined atherosclerosis regression (increased lumen volume+decreased PAV) in group A (40.5%) compared with group S (14.9%) (P=0.007). The target low-density lipoprotein cholesterol level <2mmol/L, presence of at least 4 of 5 atherosclerotic risk factors, and decreased level of vascular cellular adhesive molecule were independent predictors of plaque regression. There were no significant differences in plaque composition between the 2 groups over the study duration. However, during analysis of the 2 groups together, fibrous and fibro-fatty tissues decreased and dense calcification and necrotic core increased during follow-up. CONCLUSIONS: Dual lipid-lowering therapy starts atherosclerosis regression, but does not lead to significant changes in plaque composition. The continuous shift in plaque from fibro and fibro-fatty to necrotic with calcification was present in both groups.

• MeSH
• anticholesteremika terapeutické užití   MeSH
• aterosklerotický plát diagnostické zobrazování   farmakoterapie   patologie   MeSH
• atorvastatin MeSH
• azetidiny terapeutické užití   MeSH
• cholesterol metabolismus   MeSH
• ezetimib MeSH
• intervenční ultrasonografie MeSH
• jednoduchá slepá metoda MeSH
• kombinovaná farmakoterapie MeSH
• koronární cévy diagnostické zobrazování   metabolismus   patologie   MeSH
• kyseliny heptylové terapeutické užití   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lipoproteiny metabolismus   MeSH
• molekuly buněčné adheze metabolismus   MeSH
• nemoci koronárních tepen diagnostické zobrazování   farmakoterapie   patologie   MeSH
• progrese nemoci MeSH
• pyrroly terapeutické užití   MeSH
• senioři MeSH
• výsledek terapie MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• randomizované kontrolované studie MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• anticholesteremika MeSH
• atorvastatin MeSH
• azetidiny MeSH
• cholesterol MeSH
• ezetimib MeSH
• kyseliny heptylové MeSH
• lipoprotein cholesterol MeSH Prohlížeč
• lipoproteiny MeSH
• molekuly buněčné adheze MeSH
• pyrroly MeSH

The aim of this randomised, double-blind, placebo controlled, parallel group study was to assess the effect of trimetazidine (TMZ), a potent antiischaemic drug, on plasma C-reactive protein (C-RP), cytokine and adhesion molecule levels. The study population consists of 18 patients (16 males, 2 females, average age 56.45 +/- 10.97 years) with acute myocardial infarction admitted within 6 hours after onset of symptoms and treated with streptokinase. Blood samples were taken at 3-hour intervals during the time of treatment. All patients were randomised blindly using a centralised randomisation process, between trimetazidine (40 mg bolus i.v. then 60 mg per day for 48 hours intravenously in glucose infusion) or placebo group. Plasma C-RP level was significantly lower in TMZ group (39.5 mg/ml +/- 9.7 mg/ml) as compared to placebo (75.7 +/- 29.4 mg/ml, p < or = 0.001) and peaked 28 hours later in TMZ group. Plasma interleukin 6 (IL 6) level showed a sharp peak 9 hours after the onset of the symptoms in TMZ group (116.9 +/- 180.2 pg/ml vs. 45.4 +/- 37.9 pg/ml) and was increased up to 30 hours after the onset of the symptoms. Plasma interleukin 1 beta (IL 1 beta) was also higher in TMZ group notably 21 hours after the onset of symptoms (26.4 +/- 9.3 pg/ml vs. 16.2 +/- 2.4 pg/ml). TMZ group showed lower plasma E-selectin levels. Plasma IL 8, TNF alpha and ICAM 1 levels were without statistical significant differences. The present study demonstrates a significant reduction of plasma C-reactive protein level in the course of acute myocardial infarction treated with streptokinase and intravenous trimetazidine infusion compared with the group of patients without trimetazidine treatment.

• MeSH
• C-reaktivní protein analýza   MeSH
• cytokiny krev   MeSH
• dvojitá slepá metoda MeSH
• E-selektin krev   MeSH
• infarkt myokardu krev   farmakoterapie   MeSH
• interleukin-1 krev   MeSH
• interleukin-6 krev   MeSH
• interleukin-8 krev   MeSH
• kombinovaná terapie MeSH
• lidé středního věku MeSH
• lidé MeSH
• mezibuněčná adhezivní molekula-1 krev   MeSH
• molekuly buněčné adheze krev   MeSH
• TNF-alfa analýza   MeSH
• trimetazidin terapeutické užití   MeSH
• trombolytická terapie MeSH
• vazodilatancia terapeutické užití   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH
• práce podpořená grantem MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• C-reaktivní protein MeSH
• cytokiny MeSH
• E-selektin MeSH
• interleukin-1 MeSH
• interleukin-6 MeSH
• interleukin-8 MeSH
• mezibuněčná adhezivní molekula-1 MeSH
• molekuly buněčné adheze MeSH
• TNF-alfa MeSH
• trimetazidin MeSH
• vazodilatancia MeSH