We studied the combined effects of exposure to magnetic field (MF) 50 Hz 10 mT and X-ray ionizing radiation on developing chick embryos. When chick embryos at early stages were repeatedly exposed to MF prior to X-ray radiation, reduction of X-ray teratogenicity was observed. When MF exposures started immediately after X-ray radiation, the adverse developmental effects of ionizing radiation were potentiated. Embryotoxicity was more pronounced in X-ray irradiated and MF exposed groups. We present a hypothesis about the mechanisms of MF effect based on enhancement of oxidative processes, followed by stimulation of antioxidant systems.
- MeSH
- elektromagnetická pole škodlivé účinky MeSH
- kuřecí embryo účinky záření MeSH
- rentgenové záření škodlivé účinky MeSH
- zvířata MeSH
- Check Tag
- kuřecí embryo účinky záření MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Fungi are producers of lignolytic extracellular enzymes which are used in industries like textile, detergents, biorefineries, and paper pulping. This study assessed for the production, purification, and characterization of novel p-diphenol oxidase (PDO; laccase) enzyme from lignolytic white-rot fungal isolate. Fungi samples collected from different areas of Pakistan were initially screened using guaiacol plate method. The maximum PDO producing fungal isolate was identified on the basis of ITS (internal transcribed spacer sequence of DNA of ribosomal RNA) sequencing. To get optimum enzyme yield, various growth and fermentation conditions were optimized. Later PDO was purified using ammonium sulfate precipitation, size exclusion, and anion exchange chromatography and characterized. It was observed that the maximum PDO producing fungal isolate was Schizophyllum commune (MF-O5). Characterization results showed that the purified PDO was a monomeric protein with a molecular mass of 68 kDa and showed stability at lower temperature (30 °C) for 1 h. The Km and Vmax values of the purified PDO recorded were 2.48 mM and 6.20 U/min. Thermal stability results showed that at 30 °C PDO had 119.17 kJ/K/mol Ea value and 33.64 min half-life. The PDO activity was stimulated by Cu2+ ion at 1.0 mM showing enhanced activity up to 111.04%. Strong inhibition effect was noted for Fe2+ ions at 1 mM showing 12.04% activity. The enzyme showed stability against 10 mM concentration oxidizing reducing agents like DMSO, EDTA, H2O2, NaOCl, and urea and retained more than 75% of relative activity. The characterization of purified PDO enzyme confirmed its tolerance against salt, metal ions, organic solvents, and surfactants indicating its ability to be used in the versatile commercial applications.
- Klíčová slova
- 2′-azino-di(3-ethylbenzthiazoline-6-sulfonate), ABTS 2, Characterization, Fungi, Guaiacol, PDO (p-diphenol oxidase/laccase), Process optimization, Schizophyllum commune,
- MeSH
- koncentrace vodíkových iontů MeSH
- lakasa * metabolismus MeSH
- peroxid vodíku MeSH
- Schizophyllum * genetika metabolismus MeSH
- stabilita enzymů MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- lakasa * MeSH
- peroxid vodíku MeSH
- MeSH
- antibakteriální látky terapeutické užití MeSH
- Borrelia * MeSH
- imunoglobulin G MeSH
- imunoglobulin M MeSH
- lymeská nemoc * farmakoterapie epidemiologie veterinární MeSH
- séroepidemiologické studie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- dopisy MeSH
- komentáře MeSH
- Názvy látek
- antibakteriální látky MeSH
- imunoglobulin G MeSH
- imunoglobulin M MeSH
Cutaneous T-cell lymphomas (CTCL) represent rare non-Hodgkin lymphomas (NHL) with an incidence less than 1 per 100,000 inhabitants. The most common type of CTCL is mycosis fungoides (MF), which represents approximately 60% of all CTCL, followed by Sézary syndrome (SS), approximately 5%. We retrospectively analyzed the outcome of 118 patients with MF (n=96) and SS (n=22) treated between the years 1998 and 2021 at the Charles University General Hospital in Prague, Czech Republic. The ratio between men and women was 1.2:1 (62 men, and 56 women). The median age at diagnosis was 62 years (23 to 92 years). From the MF cohort 48 patients (50% out of MF cohort) presented with advanced stage disease. Ninety patients (77%) received a systemic treatment at any time from the diagnosis; the median number of therapy lines was two. At the time of database lock, the overall survival (OS) of 96 patients with MF reached 17.7 years with the median follow-up 4.0 years. With the median follow-up 2.6 years, the median OS of 22 patients with SS was 3.5 years. The most common type of systemic therapy for MF included low-dose methotrexate (61%), interferon-alpha (58%), bexarotene (28%), and chlorambucil (25%). The most common type of therapy for SS included bexarotene (64%), extracorporeal photopheresis (50%), and interferon-alpha (45%). Only the minority of patients received innovative targeted agents including brentuximab vedotin, mogamulizumab, or pembrolizumab. Besides the retrospective analysis of the CTCL cohort, current standards and future perspectives of selected innovative agents are summarized and discussed. The analyzed cohort represents the largest cohort of CTCL patients in the Czech Republic. Overall, the survival parameters of our CTCL cohort are comparable to those previously published by other groups. In conclusion, our analysis of 118 real world cohort of consecutive CTCL patients treated at the single center confirmed the efficacy of immune response modifiers and underlines the urgent need for ample implementation of innovative agents and their combinations into earlier lines of therapy.
- Klíčová slova
- cutaneous T-cell lymphoma (CTCL), mycosis fungoides (MF), real-world analysis, retrospective study, sézary syndrome (SS),
- Publikační typ
- časopisecké články MeSH
- Klíčová slova
- ALK (anaplastic lymphoma kinase), T-cell lymphoma, lymphoma CNS, mycosis fungoides (MF), proteomics,
- Publikační typ
- úvodníky MeSH
In therapeutic diagnostics, early diagnosis and monitoring of heart disease is dependent on fast time-series MRI data processing. Robust encryption techniques are necessary to guarantee patient confidentiality. While deep learning (DL) algorithm have improved medical imaging, privacy and performance are still hard to balance. In this study, a novel approach for analyzing homomorphivally-encrypted (HE) time-series MRI data is introduced: The Multi-Faceted Long Short-Term Memory (MF-LSTM). This method includes privacy protection. The MF-LSTM architecture protects patient's privacy while accurately categorizing and forecasting cardiac disease, with accuracy (97.5%), precision (96.5%), recall (98.3%), and F1-score (97.4%). While segmentation methods help to improve interpretability by identifying important region in encrypted MRI images, Generalized Histogram Equalization (GHE) improves image quality. Extensive testing on selected dataset if encrypted time-series MRI images proves the method's stability and efficacy, outperforming previous approaches. The finding shows that the suggested technique can decode medical image to expose visual representation as well as sequential movement while protecting privacy and providing accurate medical image evaluation.
- Klíčová slova
- Encryption, Heart Disease, MRI Images, Multi-faceted long short-term memory (MF-LSTM),
- MeSH
- algoritmy MeSH
- deep learning MeSH
- důvěrnost informací MeSH
- lidé středního věku MeSH
- lidé MeSH
- magnetická rezonanční tomografie * metody MeSH
- nemoci srdce * diagnostické zobrazování MeSH
- neuronové sítě (počítačové) MeSH
- počítačové zpracování obrazu metody MeSH
- soukromí * MeSH
- zabezpečení počítačových systémů MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Mass releases of sterilized male insects, in the frame of sterile insect technique programs, have helped suppress insect pest populations since the 1950s. In the major horticultural pests Bactrocera dorsalis, Ceratitis capitata, and Zeugodacus cucurbitae, a key phenotype white pupae (wp) has been used for decades to selectively remove females before releases, yet the gene responsible remained unknown. Here, we use classical and modern genetic approaches to identify and functionally characterize causal wp- mutations in these distantly related fruit fly species. We find that the wp phenotype is produced by parallel mutations in a single, conserved gene. CRISPR/Cas9-mediated knockout of the wp gene leads to the rapid generation of white pupae strains in C. capitata and B. tryoni. The conserved phenotype and independent nature of wp- mutations suggest this technique can provide a generic approach to produce sexing strains in other major medical and agricultural insect pests.
- MeSH
- biologická kontrola škůdců metody MeSH
- Ceratitis capitata genetika MeSH
- CRISPR-Cas systémy MeSH
- fenotyp MeSH
- fertilita genetika MeSH
- genom hmyzu genetika MeSH
- hmyzí proteiny genetika MeSH
- kukla genetika MeSH
- mutace * MeSH
- rozmnožování genetika MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- Tephritidae klasifikace genetika MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Názvy látek
- hmyzí proteiny MeSH
The rheometer curing curves of neat natural rubber (NR) and neat chloroprene rubber (CR) with maleide F (MF) exhibit considerable crosslinking torque at 180 °C. This indicates that MF can crosslink both these rubbers via Alder-ene reactions. Based on this knowledge, MF has been introduced as a co-crosslinking agent for a 50/50 blend of NR and CR in conjunction with accelerated sulfur. The delta (Δ) torque obtained from the curing curves of a blend with the addition of 1 phr MF was around 62% higher than those without MF. As the content of MF increased to 3 phr, the Δ torque was further raised to 236%. Moreover, the mechanical properties, particularly the tensile strength of the blend with the addition of 1 phr MF in conjunction with the accelerated sulfur, was around 201% higher than the blend without MF. The overall tensile properties of the blends cured with MF were almost retained even after ageing the samples at 70 °C for 72 h. This significant improvement in the curing torque and the tensile properties of the blends indicates that MF can co-crosslink between NR and CR via the Diels-Alder reaction.
- Klíčová slova
- Diels–Alder reaction, bismaleimide, curing, rubber, tensile strength,
- Publikační typ
- časopisecké články MeSH
Interactions between magnetic fields (MFs) and living cells may stimulate a large variety of cellular responses to a MF, while the underlying intracellular mechanisms still remain a great puzzle. On a fundamental level, the MF - cell interaction is affected by the two broken symmetries: (a) left-right (LR) asymmetry of the MF and (b) chirality of DNA molecules carrying electric charges and subjected to the Lorentz force when moving in a MF. Here we report on the chirality-driven effect of static magnetic fields (SMFs) on DNA synthesis. This newly discovered effect reveals how the interplay between two fundamental features of symmetry in living and inanimate nature-DNA chirality and the inherent features of MFs to distinguish the left and right-manifests itself in different DNA synthesis rates in the upward and downward SMFs, consequently resulting in unequal cell proliferation for the two directions of the field. The interplay between DNA chirality and MF LR asymmetry will provide fundamental knowledge for many MF-induced biological phenotypes.
- Klíčová slova
- DNA synthesis, biomagnetic effects, homochirality, left-right asymmetry, magnetic field,
- Publikační typ
- časopisecké články MeSH
BACKGROUND: Regular walking in different types of footwear may increase the mediolateral shear force, knee adduction moment, or vertical ground-reaction forces that could increase the risk of early development of knee osteoarthritis (OA). PURPOSE: To compare kinematic and kinetic parameters that could affect the development of knee OA in 3 footwear conditions. STUDY DESIGN: Controlled laboratory study. METHODS: A total of 40 asymptomatic participants performed walking trials in the laboratory at self-selected walking speeds under barefoot (BF), minimalistic (MF), and neutral (NF) footwear conditions. Knee joint parameters were described using discrete point values, and continuous curves were evaluated using statistical parametric mapping. A 3 × 1 repeated-measures analysis of variance was used to determine the main effect of footwear for both discrete and continuous data. To compare differences between footwear conditions, a post hoc paired t test was used. RESULTS: Discrete point analyses showed a significantly greater knee power in NF compared with MF and BF in the weight absorption phase (P < .001 for both). Statistical parametric mapping analysis indicated a significantly greater knee angle in the sagittal plane at the end of the propulsive phase in BF compared with NF and MF (P = .043). Knee joint moment was significantly greater in the propulsive phase for the sagittal (P = .038) and frontal planes (P = .035) in BF compared with NF and MF and in the absorption phase in the sagittal plane (P = .034) in BF compared with MF and NF. A significant main effect of footwear was found for anteroposterior (propulsion, ↑MF, NF, ↓BF [P = .008]; absorption, ↑BF, MF, ↓NF [P = .001]), mediolateral (propulsion, ↑MF, NF, ↓BF [P = .005]; absorption, ↑NF, MF, ↓BF [P = .044]), and vertical (propulsion, ↑NF, BF, ↓MF [P = .001]; absorption, ↑MF, BF, ↓NF [P < .001]) ground-reaction forces. Knee power showed a significant main effect of footwear (absorption, ↑NF, MF, ↓BF [P = .015]; propulsion, ↑MF, NF, ↓BF [P = .039]). CONCLUSION: Walking in MF without sufficient accommodation affected kinetic and kinematic parameters and could increase the risk of early development of knee OA.
- Klíčová slova
- SPM, barefoot, footwear, knee, minimalistic, osteoarthritis, walking,
- Publikační typ
- časopisecké články MeSH
