Titanium surface treatment is a crucial process for achieving sufficient osseointegration of an implant into the bone. If the implant does not heal sufficiently, serious complications may occur, e.g. infection, inflammation, aseptic loosening of the implant, or the stress-shielding effect, as a result of which the implant may need to be reoperated. After a titanium graft has been implanted, several interactions are crucial in order to create a strong bone-implant connection. It is essential that cells adhere to the surface of the implant. Surface roughness has a significant influence on cell adhesion, and also on improving and accelerating osseointegration. Other highly important factors are biocompatibility and resistance to bacterial contamination. Bio-inertness of titanium is ensured by the protective film of titanium oxides that forms spontaneously on its surface. This film prevents the penetration of metal compounds, and it is well-adhesive for calcium and phosphate ions, which are necessary for the formation of the mineralized bone structure. Since the presence of the film alone is not sufficient for the biocompatibility of titanium, a suitable surface finish is required to create a firm bone-implant connection. In this review, we explain and compare the most widely-used methods for modulating the surface roughness of titanium implants in order to enhance cell adhesion on the surface of the implant, e.g. plasma spraying, sandblasting, acid etching, laser treatment, sol-gel etc., The methods are divided into three overlapping groups, according to the type of modification.

• Klíčová slova
• biocompatibility, osseointegration, surface modification, titanium treatment,
• MeSH
• biokompatibilní potahované materiály * MeSH
• lidé MeSH
• osteointegrace * MeSH
• protézy a implantáty * MeSH
• titan * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• biokompatibilní potahované materiály * MeSH
• titan * MeSH

Titanium miniplates are biocompatible materials used in modern oral and maxillofacial surgery to treat facial bone fractures. However, plate removal is often required due to implant complications. Among them, a biofilm formation on an infected miniplate is associated with severe inflammation, which frequently results in implant failure. In light of this, new strategies to control or treat oral bacterial biofilm are of high interest. Herein, the authors exploit the ability of nanorobots against multispecies bacterial biofilm grown onto facial commercial titanium miniplate implants to simulate pathogenic conditions of the oral microenvironment. The strategy is based on the use of light-driven self-propelled tubular black-TiO2 /Ag nanorobots, that unlike traditional ones, exhibit an extended absorption and motion actuation from UV to the visible-light range. The motion analysis is performed separately over UV, blue, and green light irradiation and shows different motion behaviors, including a fast rotational motion that decreases with increasing wavelengths. The biomass reduction is monitored by evaluating LIVE/DEAD fluorescent and digital microscope images of bacterial biofilm treated with the nanorobots under motion/no-motion conditions. The current study and the obtained results can bring significant improvements for effective therapy of infected metallic miniplates by biofilm.

• Klíčová slova
• UV light, bacteria, black TiO 2, dental implants, nanomotors, silver, visible light,
• MeSH
• Bacteria MeSH
• biofilmy * MeSH
• protézy a implantáty MeSH
• titan * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• titan * MeSH

Formation of a calcium phosphate layer was studied on the surfaces of plasma-sprayed hydroxyapatite (PSHA) and sandblasted commercially pure (cp) titanium in simulated body fluid with ion concentrations similar to those of human blood plasma. The PSHA surface induced the formation of calcium phosphate surface layers, while the precipitation of calcium phosphate on sandblasted cp titanium was not detected. Histologic evaluation of in vivo tests demonstrated that implants with a PSHA coating enabled the growth of bone tissue into gaps with a depth of up to 1 mm without significant formation of intermediate fibrous tissue. In comparison to sandblasted cp titanium, implants with PSHA coating exhibited greater tolerance to unfavorable conditions during healing, such as gaps at the interface or primary instability of the implant. In the case of good primary stability of the implant, filling of the gap with fibrous tissue was observed for sandblasted cp titanium implants over the greater part of the surface of gaps with a depth of 0.3 mm. Direct contact of cp titanium implants with bone was achieved only when the press-fit implantation model was used.

• MeSH
• biokompatibilní potahované materiály * MeSH
• endoseální implantace zubů MeSH
• hydroxyapatit * MeSH
• leštění zubů MeSH
• lidé MeSH
• povrchové vlastnosti MeSH
• psi MeSH
• remodelace kosti * MeSH
• titan MeSH
• zubní implantáty * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• psi MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• biokompatibilní potahované materiály * MeSH
• hydroxyapatit * MeSH
• titan MeSH
• zubní implantáty * MeSH

An endosseous implant is described as osseointegrated when it is immobile in function. Objective measures of stability testing have been described. The Periotest is a commercially available device that is used for this purpose. This study was designed to measure stability of endosseous implants placed in the mandible. Implants were placed in the mandibular canine or first premolar area to support an overdenture prosthesis. Stability was evaluated through the use of a Periotest device at the time of implant placement and following one year of functional loading. Implant designs were either a screw-shaped titanium alloy or a hydroxyapatite-coated cylinder. A total of 54 implants were placed, 37 were titanium screw-shaped implants, while the remaining 17 were hydroxyapatite cylinders. Initial measurements of stability showed no difference due to implant type. Following one year of functional loading, titanium screw-shaped implants were more stable than hydroxyapatite implants (P < 0.05). The difference in implant rigidity following a period of functional loading may be an indication of a difference in osseointegration between the two implants used in this study.

• MeSH
• analýza rozptylu MeSH
• biokompatibilní potahované materiály * chemie   MeSH
• hydroxyapatit * chemie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mandibula chirurgie   MeSH
• následné studie MeSH
• neparametrická statistika MeSH
• osteointegrace * MeSH
• parodontologie přístrojové vybavení   MeSH
• pilotní projekty MeSH
• povrchové vlastnosti MeSH
• premolár MeSH
• pružnost MeSH
• retence zubní protézy přístrojové vybavení   MeSH
• senioři MeSH
• slitiny MeSH
• špičák MeSH
• statistika jako téma MeSH
• titan * chemie   MeSH
• zatížení muskuloskeletálního systému MeSH
• zubní implantáty * MeSH
• zubní náhrada ve spojení s implantáty MeSH
• zubní náhrady - překrytí MeSH
• zubní protéza - design * MeSH
• zubní slitiny chemie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biokompatibilní potahované materiály * MeSH
• hydroxyapatit * MeSH
• slitiny MeSH
• titan * MeSH
• titanium alloy (TiAl6V4) MeSH Prohlížeč
• zubní implantáty * MeSH
• zubní slitiny MeSH

AIM: The aim of this study was to use the beta-titanium alloy Ti38Nb6Ta for production of a new construction line of implants, perform testing on animals and preclinical tests. MATERIALS AND METHODS: Within this study, a new PV I implant with five construction variants was developed. The implant includes three types of threads - microthreads and flat threads of two types with a different depth. Further, the PV I implant was tested on minipigs. Subsequently, preclinical tests of 150 implants were performed and assessed. The age interval of patients was from 18 to 74 years. RESULTS: Beta titanium alloy exhibited higher strength than titanium alloys. Anti-corrosion resistance was also higher. The implant from beta-alloy was inserted in the tibias of minipigs. Sections showed good osseointegration of the PV I implant. During the preclinical tests, 150 implants were inserted with the success rate of 99.33% after the two year assessment. The assessment also included handicapped patients who are not usually assessed in classical studies. Finally, the implantation protocol and documentation of a new implantation system PV I was designed. At the same time the industrial sample of this implant was formed and accepted. CONCLUSION: A new anti-rotation PV I implant with microthreads and conical anchorage of the abutment into the fixture was formed. The beta-titanium alloy Ti38Nb6Ta used for the implant was biocompatible and had higher mechanical and physical properties than the existing titanium alloys. The PV I implant was recommended for clinical application.

• Klíčová slova
• anti-rotation feature, beta-titanium alloy, handicapped patient, microthreads,
• MeSH
• čelist bezzubá chirurgie   MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• postižení * MeSH
• protézy - design MeSH
• senioři MeSH
• testování materiálů MeSH
• titan farmakologie   MeSH
• zubní implantáty * MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• beta titanium MeSH Prohlížeč
• titan MeSH
• zubní implantáty * MeSH

The aim of this paper is to review the experimental methods currently being used to evaluate the osseointegration of nanostructured titanium implants using animal models. The material modifications are linked to the biocompatibility of various types of oral implants, such as laser-treated, acid-etched, plasma-coated, and sand-blasted surface modifications. The types of implants are reviewed according to their implantation site (endoosseous, subperiosteal, and transosseous implants). The animal species and target bones used in experimental implantology are carefully compared in terms of the ratio of compact to spongy bone. The surgical technique in animal experiments is briefly described, and all phases of the histological evaluation of osseointegration are described in detail, including harvesting tissue samples, processing undemineralized ground sections, and qualitative and quantitative histological assessment of the bone-implant interface. The results of histological staining methods used in implantology are illustrated and compared. A standardized and reproducible technique for stereological quantification of bone-implant contact is proposed and demonstrated. In conclusion, histological evaluation of the experimental osseointegration of dental implants requires careful selection of the experimental animals, bones, and implantation sites. It is also advisable to use larger animal models and older animals with a slower growth rate rather than small or growing experimental animals. Bones with a similar ratio of compact to spongy bone, such as the human maxilla and mandible, are preferred. A number of practical recommendations for the experimental procedures, harvesting of samples, tissue processing, and quantitative histological evaluations are provided.

• MeSH
• implantace protézy metody   MeSH
• modely nemocí na zvířatech MeSH
• nemoci kostí chirurgie   MeSH
• osteointegrace * MeSH
• protézy a implantáty * MeSH
• titan * MeSH
• výsledek terapie MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• titan * MeSH

The aim of this study was to investigate the absorbed dose and the linear energy transfer (LET) of a scanning proton pencil beam at the Proton Therapy Center Czech, applied to phantoms containing metal implants. We investigated two different phantoms composed of commonly used metals with a known chemical composition. Two rectangular phantoms consisted of water-equivalent environment material with a 65 mm thickness surrounding the 2, 5, 10 and 15 mm inserts of grade-2 and grade-5 Titanium. Track-etched detectors (TEDs) were placed behind the phantoms to gather the data. The measured LET spectra behind the implants were compared with Monte Carlo simulations using the Geant4 toolkit, version 10.03.p01. The simulations were used to provide additional information regarding the contribution of each type of particles to the LET spectra (protons, alpha particles, deuteron, neutrons, photons, and electrons) and to estimate the LET spectra above the TED's detection threshold. We used two different beam energies to study the most pertinent irradiation scenarios, one in the Bragg curve plateau and one at the maximum. The measurement of the LET spectra behind phantoms irradiated with a proton beam in the plateau region of the Bragg curve led to the detection of numerous particles with a very high LET. Lateral dose enhancement at the border between implants and the plastic material was detected when the phantoms were exposed to a proton beam and the data were recorded in the Bragg peak maximum. In this area, the dose increased 13 times for grade-2 Ti and 12 times for grade-5 Ti. The performed experimental study highlights the effect of dental implants on the LET spectra and absorbed dose when a proton pencil beam is crossing high-density titanium.

• MeSH
• artefakty MeSH
• fantomy radiodiagnostické MeSH
• lidé MeSH
• lineární přenos energie MeSH
• metoda Monte Carlo MeSH
• protonová terapie * MeSH
• radiometrie MeSH
• titan * MeSH
• zubní implantáty * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• titan * MeSH
• zubní implantáty * MeSH

PURPOSE: This study was carried out to quantify the effect of an alkali-modified surface on implant stability during healing using an animal model. MATERIALS AND METHODS: A total of 24 screw-shaped, self-tapping, commercially pure titanium dental implants, divided into a test group (implants with an alkali-modified surface or "biosurface") and a control group (implants with a turned, machined surface) were inserted without pretapping in the tibiae of 3 beagle dogs. The resonance frequency analysis method was used to measure the implant stability quotient (ISQ) 0, 1, 3, 9, and 12 weeks after implantation. The animals were sacrificed after 2, 5, and 12 weeks, and the bone-implant contact (BIC%) was evaluated histomorphometrically. RESULTS: The difference in the osseointegration rates (deltaISQ/deltahealing time) between the implants with alkali-modified surface (biosurface) and those with a turned, machined surface was evaluated as a mean of 0.843 ISQ/week within the first 9 weeks of healing. The mean increase in the secondary implant stability was found to be proportional to the mean increase in the BIC at healing period earlier than 5 weeks. DISCUSSION: The characteristics that differed between the implant surfaces, ie, specific surface area, contact angle, and hydroxylation/hydration, may represent factors that influence the rate of osseointegration and the secondary implant stability. CONCLUSION: The alkali-treated surface enhances the secondary stability in the early stages of healing compared to the turned, machined surface, as a consequence of faster BIC formation.

• MeSH
• alkálie MeSH
• endoseální implantace zubů MeSH
• experimentální implantáty MeSH
• hydroxidy MeSH
• leptání zubů metody   MeSH
• leštění zubů MeSH
• osteointegrace MeSH
• povrchové vlastnosti MeSH
• psi MeSH
• retence zubní protézy * MeSH
• smáčivost MeSH
• tibie MeSH
• titan MeSH
• vibrace MeSH
• zubní implantáty * MeSH
• zubní protéza - design * MeSH
• zuby-sanace - selhání MeSH
• zvířata MeSH
• Check Tag
• psi MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• alkálie MeSH
• hydroxide ion MeSH Prohlížeč
• hydroxidy MeSH
• titan MeSH
• zubní implantáty * MeSH

Orthopedic implants heal well without complications in most patients but fail for unclear reasons in some individuals. This study determined the relevance of metal hypersensitivity in patients with failed orthopedic implants and those requiring orthopedic implant surgery. The study included 35 patients with failed orthopedic implants and 15 subjects scheduled for orthopedic implant surgery. The production of selected pro-inflammatory cytokines was measured in patients with failed orthopedic implants. Metal hypersensitivity was measured in all subjects using the MELISA® test. Of common metals in orthopedic alloys, the patients with failed orthopedic implants responded most frequently to nickel, chromium, titanium, iron, and molybdenum. Hypersensitivity to metals found in implants was measured in 40% of patients with failed implants. The study also showed that titanium exposure in patients with titanium hypersensitivity might lead to implant failure. Metal hypersensitivity testing should be offered to patients before surgery to minimize the risk of implant failure.

• Klíčová slova
• Cytokines, Implant failure, MELISA®, Metal hypersensitivity, Orthopedic implants,
• MeSH
• alergie * MeSH
• cytokiny MeSH
• kovy škodlivé účinky   MeSH
• lidé MeSH
• protézy a implantáty škodlivé účinky   MeSH
• studie případů a kontrol MeSH
• titan * škodlivé účinky   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• cytokiny MeSH
• kovy MeSH
• titan * MeSH

Anodization coloring of titanium tools or implants is one of the common methods for the differentiation of each application by its size or type. Commercial purity titanium grade 4 plates (50 × 20 × 0.1 mm) were tested to obtain their electrochemical and other technological properties. The coloring process was done using the potential of 15, 30, 45, 60, and 75 Volts for 5 s in 1 wt. % citric acid in demineralized water solution. Organic acids solutions generally produce better surface quality compared to inorganic acids. The contact angle of colored surfaces was measured by the sessile drop method. Electrochemical impedance spectroscopy and potentiodynamic polarization were used for the determination of selected electrochemical and corrosion parameters of the tested surfaces. It was found that the anodization process decreases corrosion potential significantly. It was also confirmed that a higher potential used for anodization results in higher polarization resistance but also a decrease in corrosion potential. The anodization process at 75 V produces surfaces with the lowest corrosion rate under 1 nm/year and the noblest corrosion potential. It was confirmed that the anodization process in citric acid does not affect titanium cytotoxicity.

• Klíčová slova
• anodization, biocompatibility, corrosion properties, polarization, titanium,
• Publikační typ
• časopisecké články MeSH