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SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis
VP. Ferreira, V. Fazito Vale, MK. Pangburn, M. Abdeladhim, AF. Mendes-Sousa, IV. Coutinho-Abreu, M. Rasouli, EA. Brandt, C. Meneses, KF. Lima, R. Nascimento Araújo, MH. Pereira, M. Kotsyfakis, F. Oliveira, S. Kamhawi, JM. Ribeiro, NF. Gontijo, N....
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2011
Free Medical Journals
od 2011
Nature Open Access
od 2011-12-01
PubMed Central
od 2011
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od 2011
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od 2011-01-01
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od 2011-01-01
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od 2011-01-01
Health & Medicine (ProQuest)
od 2011-01-01
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od 2011
Springer Nature OA/Free Journals
od 2011-12-01
PubMed
26758086
DOI
10.1038/srep19300
Knihovny.cz E-zdroje
- MeSH
- aktivace komplementu účinky léků MeSH
- hmyzí proteiny farmakologie MeSH
- inhibitory komplementu farmakologie MeSH
- klasická dráha komplementu účinky léků MeSH
- komplement 4 antagonisté a inhibitory imunologie metabolismus MeSH
- komplement C1 antagonisté a inhibitory imunologie metabolismus MeSH
- lidé MeSH
- Psychodidae imunologie metabolismus MeSH
- rekombinantní proteiny farmakologie MeSH
- sliny metabolismus MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Research Support, N.I.H., Intramural MeSH
Blood-feeding insects inject potent salivary components including complement inhibitors into their host's skin to acquire a blood meal. Sand fly saliva was shown to inhibit the classical pathway of complement; however, the molecular identity of the inhibitor remains unknown. Here, we identified SALO as the classical pathway complement inhibitor. SALO, an 11 kDa protein, has no homology to proteins of any other organism apart from New World sand flies. rSALO anti-complement activity has the same chromatographic properties as the Lu. longipalpis salivary gland homogenate (SGH)counterparts and anti-rSALO antibodies blocked the classical pathway complement activity of rSALO and SGH. Both rSALO and SGH inhibited C4b deposition and cleavage of C4. rSALO, however, did not inhibit the protease activity of C1s nor the enzymatic activity of factor Xa, uPA, thrombin, kallikrein, trypsin and plasmin. Importantly, rSALO did not inhibit the alternative or the lectin pathway of complement. In conclusion our data shows that SALO is a specific classical pathway complement inhibitor present in the saliva of Lu. longipalpis. Importantly, due to its small size and specificity, SALO may offer a therapeutic alternative for complement classical pathway-mediated pathogenic effects in human diseases.
Biology Center of the Czech Academy of Sciences Budweis CZ 370 05 Czech Republic
The University of Texas Health Science Center at Tyler Tyler TX
Vector Biology Section LMVR National Institute of Allergy and Infectious Diseases NIH Rockville MD
Citace poskytuje Crossref.org
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