Escherichia coli strain with a deletion of the chromosomal ampC gene marked with TcR, suitable for production of penicillin G acylase
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11097022
DOI
10.1007/bf02825651
Knihovny.cz E-resources
- MeSH
- Genes, Bacterial * MeSH
- Bacterial Proteins * MeSH
- Bacteriophage P1 genetics MeSH
- beta-Lactamases genetics MeSH
- Gene Deletion MeSH
- Escherichia coli drug effects enzymology genetics MeSH
- Genetic Markers MeSH
- Penicillin Amidase biosynthesis genetics MeSH
- Plasmids genetics MeSH
- Recombinant Proteins biosynthesis genetics MeSH
- Tetracycline Resistance genetics MeSH
- Transduction, Genetic MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- AmpC beta-lactamases MeSH Browser
- Bacterial Proteins * MeSH
- beta-Lactamases MeSH
- Genetic Markers MeSH
- Penicillin Amidase MeSH
- Recombinant Proteins MeSH
Escherichia coli strain which contains a marker of tetracycline resistance gene (TcR) placed by P1 transduction beside the chromosomal deletion of ampC gene (delta ampC) coding for beta-lactamase was constructed. Such introduction of TcR marker permits a fast and simple selection for the transfer of delta ampC by P1 transduction into industrial E. coli strains. This approach was used for constructing an E. coli strain suitable for penicillin acylase production.
See more in PubMed
Annu Rev Microbiol. 1992;46:461-95 PubMed
J Appl Bacteriol. 1992 Jul;73(1):14-22 PubMed
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1111-5 PubMed
Gene. 1991 Apr;100:189-94 PubMed
Folia Microbiol (Praha). 1999;44(3):263-6 PubMed
Folia Microbiol (Praha). 1998;43(6):601-4 PubMed
Gene. 1993 May 15;127(1):47-52 PubMed
Science. 1983 Feb 11;219(4585):703-9 PubMed
J Mol Biol. 1976 Jul 5;104(3):541-55 PubMed
FEMS Microbiol Lett. 1993 Dec 15;114(3):349-54 PubMed
Gene replacement techniques for Escherichia coli genome modification
