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Antibiotic resistance and virulence factors among clinical and food enterococci isolated in Slovakia

Folia microbiologica. 2004 ; 49 (6) : 763-8.

Folia Microbiol (Praha)
ISSN 0015-5632
Source

Language English Country United States Media print

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid15881416

PubMed 15881416
DOI 10.1007/bf02931562
Knihovny.cz E-resources

MeSH
Anti-Bacterial Agents pharmacology MeSH
Drug Resistance, Bacterial * MeSH
Bacterial Proteins genetics metabolism MeSH
Enterococcus faecalis drug effects pathogenicity MeSH
Enterococcus faecium drug effects pathogenicity MeSH
Enterococcus classification drug effects isolation & purification pathogenicity MeSH
Virulence Factors genetics metabolism MeSH
Gram-Positive Bacterial Infections microbiology MeSH
Humans MeSH
Microbial Sensitivity Tests MeSH
Sheep MeSH
Cheese microbiology MeSH
Animals MeSH
Check Tag
Humans MeSH
Animals MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Geographicals
Slovakia MeSH
Names of Substances
Anti-Bacterial Agents MeSH
Bacterial Proteins MeSH
Virulence Factors MeSH

The resistance to antibiotics and the distribution of virulence factors in enterococci isolated from traditional Slovak sheep cheese bryndza was compared with strains from human infections. The occurrence of 4 enterococcal species was observed in 117 bryndza-cheese isolates. The majority of strains were identified as E. faecium (76 %) and E. faecalis (23 %). Several strains of E. durans and 1 strain of E. hirae were also present. More than 90 % of strains isolated from 109 clinical enterococci were E. faecalis, the rest belonged to E. faecium. The resistance to 6 antimicrobial substances (ampicillin, ciprofloxacin, higher concentration of gentamicin, nitrofurantoin, tetracycline and vancomycin) was tested in clinical and food enterococci. A higher level of resistance was found in clinical than in food strains and E. faecium had a higher resistance than E. faecalis; no resistance to vancomycin was detected. The occurrence of 3 virulence-associated genes, cylA (coding for hemolysin), gelE (coding for gelatinase) and esp (coding for surface protein) was monitored. Differences were found in the distribution of cylA gene between clinical and bryndza-cheese E. faecalis strains; in contrast to clinical strains (45 %), cylA gene was detected in 22 % of food isolates. The distribution of 2 other virulence factors, gelE and esp, was not significantly different in the two groups of E. faecalis strains. cylA and gelE genes were not detected in E. faecium but more than 70 % of clinical E. faecium were positive for esp, even thought none of the 79 E. faecium cheese isolates contained this gene.

Department of Molecular Biology Faculty of Science Comenius University Bratislava Slovakia

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