The role of intrarenal angiotensin II in the development of hypertension in Ren-2 transgenic rats
Language English Country Netherlands Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
16003180
DOI
10.1097/01.hjh.0000174972.46663.5e
PII: 00004872-200508000-00016
Knihovny.cz E-resources
- MeSH
- Angiotensin II blood metabolism MeSH
- Antihypertensive Agents pharmacology MeSH
- Benzimidazoles pharmacology MeSH
- Biphenyl Compounds MeSH
- Animals, Genetically Modified MeSH
- Hypertension genetics metabolism MeSH
- Blood Pressure drug effects genetics MeSH
- Rats MeSH
- Kidney blood supply chemistry MeSH
- Rats, Sprague-Dawley MeSH
- Proteins analysis metabolism MeSH
- Radioimmunoassay MeSH
- Renal Circulation drug effects MeSH
- Sodium urine MeSH
- Tetrazoles pharmacology MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Angiotensin II MeSH
- Antihypertensive Agents MeSH
- Benzimidazoles MeSH
- Biphenyl Compounds MeSH
- candesartan MeSH Browser
- Proteins MeSH
- Sodium MeSH
- Tetrazoles MeSH
OBJECTIVE: We investigated the responses of mean arterial pressure and renal blood flow to intravenous and intrarenal angiotensin II, plasma and kidney angiotensin II concentrations and renal angiotensin receptor subtype 1 protein expression, and renal functional responses to intravenous and intrarenal angiotensin receptor 1 blockade with candesartan. METHODS: In male anaesthetized transgenic rats and Hannover Sprague-Dawley rats aged 36-38 days mean arterial pressure and renal blood flow were determined after intravenous and intrarenal boluses of angiotensin II. Mean arterial pressure, renal blood flow and sodium excretion after intravenous or intrarenal candesartan were studied. Plasma and kidney angiotensin II concentrations were determined by radioimmunoassay. Renal angiotensin receptor subtype 1 protein levels were analysed by immunoblotting. RESULTS: The responses of mean arterial pressure and renal blood flow to angiotensin II were significantly greater in transgenic than in Hannover Sprague-Dawley rats. The administration of candesartan resulted in comparable decreases in mean arterial pressure and increases in renal blood flow and sodium excretion in both groups of rats. Renal angiotensin receptor subtype 1 protein levels were no different between Hannover Sprague-Dawley and transgenic rats. CONCLUSIONS: Plasma and kidney angiotensin II levels were lower in anaesthetized transgenic rats but, in contrast, were higher in decapitated transgenic rats when compared with Hannover Sprague-Dawley rats, suggesting that the kidney function of prehypertensive transgenic rats is under inappropriately high angiotensin II-dependent influence.
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