Evaluation of reference genes and normalization strategy for quantitative real-time PCR in human pancreatic carcinoma
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22377737
PubMed Central
PMC3826910
DOI
10.3233/dma-2011-0875
PII: A1240J248P001128
Knihovny.cz E-resources
- MeSH
- Eukaryotic Initiation Factor-2B genetics MeSH
- Gene Expression MeSH
- Nuclear Proteins genetics MeSH
- Carcinoma diagnosis genetics pathology MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Middle Aged MeSH
- Humans MeSH
- Mitochondrial Proteins genetics MeSH
- Biomarkers, Tumor genetics MeSH
- Pancreatic Neoplasms diagnosis genetics pathology MeSH
- Pancreas metabolism pathology MeSH
- Ribonucleases genetics MeSH
- Ribonucleoproteins genetics MeSH
- Ribosomal Proteins genetics MeSH
- Aged MeSH
- Software MeSH
- RNA Stability MeSH
- Gene Expression Profiling MeSH
- Transcription Factors genetics MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- ELF1 protein, human MeSH Browser
- Eukaryotic Initiation Factor-2B MeSH
- Nuclear Proteins MeSH
- Mitochondrial Proteins MeSH
- MRPL19 protein, human MeSH Browser
- Biomarkers, Tumor MeSH
- POP4 protein, human MeSH Browser
- Ribonucleases MeSH
- Ribonucleoproteins MeSH
- Ribosomal Proteins MeSH
- Transcription Factors MeSH
Histologically verified pairs (n=10) of pancreatic tumors and non-neoplastic tissues were used for quantitative real-time PCR and the stability of 24 reference genes was analyzed with geNorm and NormFinder software. Raw C{q} values correlated with the degree of RNA degradation. This correlation was abolished by normalization to C{q} of 18S endogenous control gene. Both geNorm and NormFinder programs suggested EIF2B1, ELF1, MRPL19, and POP4 as the same most stable genes. We have thus identified suitable reference genes for future expression studies in pancreatic carcinoma. Normalization method reducing the effects of RNA degradation on the quality of results was also developed.
References provided by Crossref.org
KRAS pathway expression changes in pancreatic cancer models by conventional and experimental taxanes
Hedgehog pathway overexpression in pancreatic cancer is abrogated by new-generation taxoid SB-T-1216
