Genome analysis in many plant species is hampered by large genome size and by sequence redundancy due to the presence of repetitive DNA and polyploidy. One solution is to reduce the sample complexity by dissecting the genomes to single chromosomes. This can be realized by flow cytometric sorting, which enables purification of chromosomes in large numbers. Coupling the chromosome sorting technology with next generation sequencing provides a targeted and cost effective way to tackle complex genomes. The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing.

Centre of the Region Haná for Biotechnological and Agricultural Research Institute of Experimental Botany Sokolovská 6 CZ 77200 Olomouc Czech Republic

Citace poskytuje Crossref.org

The complex polyploid genome architecture of sugarcane

Visualizing Chromosome Territories and Nuclear Architecture of Large Plant Genomes Using Alien Introgressions

Kinetics of DNA Repair in Vicia faba Meristem Regeneration Following Replication Stress

A flow cytometry-based analysis to establish a cell cycle synchronization protocol for Saccharum spp

Flow cytometric characterisation of the complex polyploid genome of Saccharum officinarum and modern sugarcane cultivars

Instability of Alien Chromosome Introgressions in Wheat Associated with Improper Positioning in the Nucleus

Fine Mapping of Lr49 Using 90K SNP Chip Array and Flow-Sorted Chromosome Sequencing in Wheat

Development of Deletion Lines for Chromosome 3D of Bread Wheat

Characterization of repetitive DNA landscape in wheat homeologous group 4 chromosomes