Bacteriophages of Staphylococcus aureus efficiently package various bacterial genes and mobile genetic elements including SCCmec with different frequencies
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- bakteriální chromozomy genetika MeSH
- bakteriální geny * MeSH
- bakteriální proteiny genetika MeSH
- bakteriofágy genetika metabolismus MeSH
- DNA bakterií genetika MeSH
- frekvence genu MeSH
- genetické lokusy MeSH
- klonování DNA MeSH
- penicilinasa genetika MeSH
- plazmidy genetika MeSH
- polymerázová řetězová reakce MeSH
- přenos genů horizontální MeSH
- proteiny vázající penicilin MeSH
- rezistence na methicilin genetika MeSH
- rozptýlené repetitivní sekvence * MeSH
- sekvenční analýza DNA MeSH
- sestavení viru MeSH
- Staphylococcus aureus genetika fyziologie virologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny MeSH
- DNA bakterií MeSH
- mecA protein, Staphylococcus aureus MeSH Prohlížeč
- penicilinasa MeSH
- proteiny vázající penicilin MeSH
Staphylococcus aureus is a serious human and veterinary pathogen in which new strains with increasing virulence and antimicrobial resistance occur due to acquiring new genes by horizontal transfer. It is generally accepted that temperate bacteriophages play a major role in gene transfer. In this study, we proved the presence of various bacterial genes of the S. aureus COL strain directly within the phage particles via qPCR and quantified their packaging frequency. Non-parametric statistical analysis showed that transducing bacteriophages φ11, φ80 and φ80α of serogroup B, in contrast to serogroup A bacteriophage φ81, efficiently package selected chromosomal genes localized in 4 various loci of the chromosome and 8 genes carried on variable elements, such as staphylococcal cassette chromosome SCCmec, staphylococcal pathogenicity island SaPI1, genomic islands vSaα and vSaβ, and plasmids with various frequency. Bacterial gene copy number per ng of DNA isolated from phage particles ranged between 1.05 × 10(2) for the tetK plasmid gene and 3.86 × 10(5) for the SaPI1 integrase gene. The new and crucial finding that serogroup B bacteriophages can package concurrently ccrA1 (1.16 × 10(4)) and mecA (1.26 × 10(4)) located at SCCmec type I into their capsids indicates that generalized transduction plays an important role in the evolution and emergence of new methicillin-resistant clones.
Citace poskytuje Crossref.org
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