The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24715459
DOI
10.1242/dev.105940
PII: 141/8/1694
Knihovny.cz E-resources
- Keywords
- Centromere, Chromosome bridging, Mice, Nucleolus precursor body, Replication stress, Satellite DNA,
- MeSH
- Blastocyst cytology metabolism MeSH
- Cell Nucleolus metabolism MeSH
- Centromere metabolism MeSH
- Embryo, Mammalian cytology metabolism MeSH
- Transcription, Genetic MeSH
- Genome genetics MeSH
- Heterochromatin genetics MeSH
- RNA, Messenger genetics metabolism MeSH
- Microsatellite Repeats genetics MeSH
- Minisatellite Repeats genetics MeSH
- Mice MeSH
- Oocytes cytology metabolism MeSH
- RNA Processing, Post-Transcriptional genetics MeSH
- RNA Precursors genetics MeSH
- Recombination, Genetic genetics MeSH
- DNA Replication genetics MeSH
- Chromatin Assembly and Disassembly genetics MeSH
- RNA, Ribosomal biosynthesis genetics MeSH
- S Phase genetics MeSH
- Chromosomes, Mammalian metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Heterochromatin MeSH
- RNA, Messenger MeSH
- RNA Precursors MeSH
- RNA, Ribosomal MeSH
The oocyte (maternal) nucleolus is essential for early embryonic development and embryos originating from enucleolated oocytes arrest at the 2-cell stage. The reason for this is unclear. Surprisingly, RNA polymerase I activity in nucleolus-less mouse embryos, as manifested by pre-rRNA synthesis, and pre-rRNA processing are not affected, indicating an unusual role of the nucleolus. We report here that the maternal nucleolus is indispensable for the regulation of major and minor satellite repeats soon after fertilisation. During the first embryonic cell cycle, absence of the nucleolus causes a significant reduction in major and minor satellite DNA by 12% and 18%, respectively. The expression of satellite transcripts is also affected, being reduced by more than half. Moreover, extensive chromosome bridging of the major and minor satellite sequences was observed during the first mitosis. Finally, we show that the absence of the maternal nucleolus alters S-phase dynamics and causes abnormal deposition of the H3.3 histone chaperone DAXX in pronuclei of nucleolus-less zygotes.
References provided by Crossref.org
Improving the Quality of Oocytes with the Help of Nucleolotransfer Therapy
Nucleoli in embryos: a central structural platform for embryonic chromatin remodeling?
