High prevalence of ST131 among CTX-M-producing Escherichia coli from community-acquired infections, in the Czech Republic
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25188031
DOI
10.1089/mdr.2014.0070
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Genes, Bacterial MeSH
- beta-Lactamases genetics MeSH
- Clone Cells MeSH
- Disk Diffusion Antimicrobial Tests MeSH
- Child MeSH
- DNA, Bacterial genetics MeSH
- Adult MeSH
- Escherichia coli drug effects enzymology genetics isolation & purification pathogenicity MeSH
- Fluoroquinolones pharmacology MeSH
- Phylogeny MeSH
- Escherichia coli Infections epidemiology microbiology MeSH
- Community-Acquired Infections epidemiology microbiology MeSH
- Infant MeSH
- Conjugation, Genetic MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Drug Resistance, Multiple, Bacterial genetics MeSH
- Multilocus Sequence Typing MeSH
- Plasmids genetics MeSH
- Child, Preschool MeSH
- Prevalence MeSH
- Escherichia coli Proteins genetics MeSH
- Electrophoresis, Gel, Pulsed-Field MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Virulence genetics MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Child, Preschool MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- beta-lactamase CTX-M, E coli MeSH Browser
- beta-Lactamases MeSH
- DNA, Bacterial MeSH
- Fluoroquinolones MeSH
- Escherichia coli Proteins MeSH
A total of 2,683 nonrepetitive Escherichia coli isolates were collected from microbiological laboratories covering all regions of the Czech Republic, during April 2011. Antimicrobial susceptibility patterns of E. coli were assessed. All 38 cefotaxime-resistant (CTX-R) isolates were found to be extended-spectrum β-lactamase (ESBL)-positive by the double-disc synergy test. Thirty-two of those isolates produced enzymes of CTX-M-1 family, five of CTX-M-9 family, and one isolate both CTX-M types. Genotyping by multilocus sequence typing classified all ESBL-producing isolates into 13 sequence types (STs). ST131 was the most prevalent and was exclusively correlated with E. coli belonging to the more-virulent phylogroup B2. blaCTX-M-15 and blaCTX-M-9-like genes were mainly carried by plasmids belonging to the IncF group, while replicon I1 was predominant among CTX-M-1-encoding plasmids. Additionally, 63% of the ESBL-producing isolates were also resistant to ciprofloxacin. Sequence analysis of quinolone resistance-determining regions of gyrA and parC revealed the presence of amino acid substitutions in 22 out of 23 ciprofloxacin-resistant isolates. The acc(6')-Ib-cr and qnrB1 plasmid-mediated quinolone resistance genes were also detected in some of the isolates. This is the first report on the emergence and spread of CTX-M-producing E. coli in the community of the Czech Republic, indicating the high prevalence of ST131 clone among CTX-M producers.
References provided by Crossref.org
Characterization of NDM-Encoding Plasmids From Enterobacteriaceae Recovered From Czech Hospitals
