Paramagnetic nanoparticles as a platform for FRET-based sarcosine picomolar detection
Language English Country England, Great Britain Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25746688
PubMed Central
PMC4352859
DOI
10.1038/srep08868
PII: srep08868
Knihovny.cz E-resources
- MeSH
- Dextrans chemistry ultrastructure MeSH
- Humans MeSH
- Magnetite Nanoparticles chemistry ultrastructure MeSH
- Molecular Imaging methods MeSH
- Antibodies, Monoclonal chemistry immunology MeSH
- Biomarkers, Tumor analysis MeSH
- Cell Line, Tumor MeSH
- Prostatic Neoplasms chemistry diagnosis immunology MeSH
- Nanocapsules chemistry ultrastructure MeSH
- Reproducibility of Results MeSH
- Fluorescence Resonance Energy Transfer methods MeSH
- Sarcosine analysis immunology MeSH
- Sensitivity and Specificity MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Dextrans MeSH
- ferumoxtran-10 MeSH Browser
- Magnetite Nanoparticles MeSH
- Antibodies, Monoclonal MeSH
- Biomarkers, Tumor MeSH
- Nanocapsules MeSH
- Sarcosine MeSH
Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Förster resonance energy transfer (FRET). The FRET biosensor employs anti-sarcosine antibodies immobilized on paramagnetic nanoparticles surface for specific antigen binding. Successful binding of sarcosine leads to assembly of a sandwich construct composed of anti-sarcosine antibodies keeping the Förster distance (Ro) of FRET pair in required proximity. The detection is based on spectral overlap between gold-functionalized green fluorescent protein and antibodies@quantum dots bioconjugate (λex 400 nm). The saturation curve of sarcosine based on FRET efficiency (F₆₀₄/F₅₁₀ ratio) was tested within linear dynamic range from 5 to 50 nM with detection limit down to 50 pM. Assembled biosensor was then successfully employed for sarcosine quantification in prostatic cell lines (PC3, 22Rv1, PNT1A), and urinary samples of prostate adenocarcinoma patients.
See more in PubMed
Siegel R., Naishadham D. & Jemal A. Cancer statistics, 2013. CA-Cancer J Clin 63, 11–30 (2013). PubMed
Cernei N. et al. Sarcosine as a Potential Prostate Cancer Biomarker-A Review. Int J Mol Sci 14, 13893–13908 (2013). PubMed PMC
Heger Z. et al. Determination of common urine substances as an assay for improving prostate carcinoma diagnostics. Oncol Rep 31, 1846–1854 (2014). PubMed
Sreekumar A. et al. Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression. Nature 457, 910–914 (2009). PubMed PMC
Jiang Y. Q., Cheng X. L., Wang C. A. & Ma Y. F. Quantitative Determination of Sarcosine and Related Compounds in Urinary Samples by Liquid Chromatography with Tandem Mass Spectrometry. Anal Chem 82, 9022–9027 (2010). PubMed
Riedel M. et al. Photoelectrochemical Sensor Based on Quantum Dots and Sarcosine Oxidase. ChemPhysChem 14, 2338–2342 (2013). PubMed
Burton C., Gamagedara S. & Ma Y. F. A novel enzymatic technique for determination of sarcosine in urine samples. Anal Methods 4, 141–146 (2012).
Lan J. M. et al. Colorimetric determination of sarcosine in urine samples of prostatic carcinoma by mimic enzyme palladium nanoparticles. Anal Chim Acta 825, 63–68 (2014). PubMed
Chen J., Zhang J., Zhang W. P. & Chen Z. L. Sensitive determination of the potential biomarker sarcosine for prostate cancer by LC-MS with N,N '-dicyclohexylcarbodiimide derivatization. J Sep Sci 37, 14–19 (2014). PubMed
Haviv A. H., Greneche J. M. & Lellouche J. P. Aggregation Control of Hydrophilic Maghemite (gamma-Fe2O3) Nanoparticles by Surface Doping Using Cerium Atoms. J Am Chem Soc 132, 12519–12521 (2010). PubMed
Guo Q. J., Teng X. W., Rahman S. & Yang H. Patterned Langmuir-Blodgett films of mondisperse nanoparticles of iron oxide using soft lithography. J Am Chem Soc 125, 630–631 (2003). PubMed
El-Boubbou K., Gruden C. & Huang X. Magnetic glyco-nanoparticles: A unique tool for rapid pathogen detection, decontamination, and strain differentiation. J Am Chem Soc 129, 13392–13393 (2007). PubMed
Schwegmann H., Feitz A. J. & Frimmel F. H. Influence of the zeta potential on the sorption and toxicity of iron oxide nanoparticles on S. cerevisiae and E. coli. J Colloid Interface Sci 347, 43–48 (2010). PubMed
Hodek P. et al. Optimized Protocol of Chicken Antibody (IgY) Purification Providing Electrophoretically Homogenous Preparations. Int J Electrochem Sci 8, 113–124 (2013).
Shaner N. C., Steinbach P. A. & Tsien R. Y. A guide to choosing fluorescent proteins. Nat Methods 2, 905–909 (2005). PubMed
Bale S. S. et al. Nanoparticle-Mediated Cytoplasmic Delivery of Proteins To Target Cellular Machinery. ACS Nano 4, 1493–1500 (2010). PubMed
Love J. C. et al. Self-assembled monolayers of thiolates on metals as a form of nanotechnology. Chem Rev 105, 1103–1169 (2005). PubMed
Qu L. H. & Peng X. G. Control of photoluminescence properties of CdSe nanocrystals in growth. J Am Chem Soc 124, 2049–2055 (2002). PubMed
Janu L. et al. Electrophoretic study of peptide-mediated quantum dot-human immunoglobulin bioconjugation. Electrophoresis 34, 2725–2732 (2013). PubMed
Casanova D. et al. Single lanthanide-doped oxide nanoparticles as donors in fluorescence resonance energy transfer experiments. J Phys Chem B 110, 19264–19270 (2006). PubMed
Khan A. P. et al. The Role of Sarcosine Metabolism in Prostate Cancer Progression. Neoplasia 15, 491–501 (2013). PubMed PMC
Ryan D., Robards K., Prenzler P. D. & Kendall M. Recent and potential developments in the analysis of urine: A review. Anal Chim Acta 684, 17–29 (2011). PubMed
Abate-Shen C. & Shen M. M. Diagnostics The prostate-cancer metabolome. Nature 457, 799–800 (2009). PubMed
Zitka O. et al. Microfluidic chip coupled with modified paramagnetic particles for sarcosine isolation in urine. Electrophoresis 34, 2639–2647 (2013). PubMed
A Rapid Method for the Detection of Sarcosine Using SPIONs/Au/CS/SOX/NPs for Prostate Cancer Sensing
DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA