Characterisation of an unusual telomere motif (TTTTTTAGGG)n in the plant Cestrum elegans (Solanaceae), a species with a large genome
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25828846
DOI
10.1111/tpj.12839
Knihovny.cz E-resources
- Keywords
- Cestrum elegans, GenBank KM573817-573822, NGS analysis, RepeatExplorer, telomerase, telomeric sequence, unusual telomere,
- MeSH
- Cestrum genetics MeSH
- Chromosomes, Plant genetics MeSH
- Telomere chemistry genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The characterization of unusual telomere sequence sheds light on patterns of telomere evolution, maintenance and function. Plant species from the closely related genera Cestrum, Vestia and Sessea (family Solanaceae) lack known plant telomeric sequences. Here we characterize the telomere of Cestrum elegans, work that was a challenge because of its large genome size and few chromosomes (1C 9.76 pg; n = 8). We developed an approach that combines BAL31 digestion, which digests DNA from the ends and chromosome breaks, with next-generation sequencing (NGS), to generate data analysed in RepeatExplorer, designed for de novo repeats identification and quantification. We identify an unique repeat motif (TTTTTTAGGG)n in C. elegans, occurring in ca. 30 400 copies per haploid genome, averaging ca. 1900 copies per telomere, and synthesized by telomerase. We demonstrate that the motif is synthesized by telomerase. The occurrence of an unusual eukaryote (TTTTTTAGGG)n telomeric motif in C. elegans represents a switch in motif from the 'typical' angiosperm telomere (TTTAGGG)n . That switch may have happened with the divergence of Cestrum, Sessea and Vestia. The shift in motif when it arose would have had profound effects on telomere activity. Thus our finding provides a unique handle to study how telomerase and telomeres responded to genetic change, studies that will shed more light on telomere function.
References provided by Crossref.org
TeloBase: a community-curated database of telomere sequences across the tree of life
Telomeric DNA sequences in beetle taxa vary with species richness
Origin and Fates of TERT Gene Copies in Polyploid Plants
Composition and Function of Telomerase-A Polymerase Associated with the Origin of Eukaryotes
Origin, Diversity, and Evolution of Telomere Sequences in Plants
Telomerase RNAs in land plants
Comparative Dissection of Three Giant Genomes: Allium cepa, Allium sativum, and Allium ursinum
Telomeres in Plants and Humans: Not So Different, Not So Similar
Telomere- and Telomerase-Associated Proteins and Their Functions in the Plant Cell