A leucine aminopeptidase is involved in kinetoplast DNA segregation in Trypanosoma brucei

. 2017 Apr ; 13 (4) : e1006310. [epub] 20170407

Jazyk angličtina Země Spojené státy americké Médium electronic-ecollection

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/pmid28388690

Grantová podpora
203134/Z/16/Z Wellcome Trust - United Kingdom
MR/K008749/1 Medical Research Council - United Kingdom
MR/N010558/1 Medical Research Council - United Kingdom
MR/P009018/1 Medical Research Council - United Kingdom

Odkazy

PubMed 28388690
PubMed Central PMC5397073
DOI 10.1371/journal.ppat.1006310
PII: PPATHOGENS-D-16-01055
Knihovny.cz E-zdroje

The kinetoplast (k), the uniquely packaged mitochondrial DNA of trypanosomatid protists is formed by a catenated network of minicircles and maxicircles that divide and segregate once each cell cycle. Although many proteins involved in kDNA replication and segregation are now known, several key steps in the replication mechanism remain uncharacterized at the molecular level, one of which is the nabelschnur or umbilicus, a prominent structure which in the mammalian parasite Trypanosoma brucei connects the daughter kDNA networks prior to their segregation. Here we characterize an M17 family leucyl aminopeptidase metalloprotease, termed TbLAP1, which specifically localizes to the kDNA disk and the nabelschur and represents the first described protein found in this structure. We show that TbLAP1 is required for correct segregation of kDNA, with knockdown resulting in delayed cytokinesis and ectopic expression leading to kDNA loss and decreased cell proliferation. We propose that TbLAP1 is required for efficient kDNA division and specifically participates in the separation of daughter kDNA networks.

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