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W-enriched satellite sequence in the Indian meal moth, Plodia interpunctella (Lepidoptera, Pyralidae)

Dalíková, Martina
Author Dalíková, Martina Faculty of Science, University of South Bohemia, Branišovská 31, 37005, České Budějovice, Czech Republic Institute of Entomology, Biology Centre CAS, České Budějovice, Czech Republic
Zrzavá, Magda
Author Zrzavá, Magda ORCID Faculty of Science, University of South Bohemia, Branišovská 31, 37005, České Budějovice, Czech Republic. vitkova@entu.cas.cz Institute of Entomology, Biology Centre CAS, České Budějovice, Czech Republic. vitkova@entu.cas.cz
Kubíčková, Svatava
Author Kubíčková, Svatava Veterinary Research Institute, Brno, Czech Republic
Marec, František
Author Marec, František Faculty of Science, University of South Bohemia, Branišovská 31, 37005, České Budějovice, Czech Republic Institute of Entomology, Biology Centre CAS, České Budějovice, Czech Republic

Chromosome research. 2017 Oct ; 25 (3-4) : 241-252. [epub] 20170512

Chromosome Res
ISSN 1573-6849 | 0967-3849
Source

Language English Country Netherlands Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid28500471

PubMed 28500471
DOI 10.1007/s10577-017-9558-8
PII: 10.1007/s10577-017-9558-8
Knihovny.cz E-resources

Keywords
Lepidoptera, Plodia interpunctella, Satellite DNA, Sex chromosomes, Transcription, W chromosome,
MeSH
Genome, Insect MeSH
In Situ Hybridization, Fluorescence MeSH
Cloning, Molecular MeSH
Moths genetics MeSH
Sex Chromosomes * MeSH
DNA, Satellite * MeSH
Animals MeSH
Check Tag
Animals MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Names of Substances
DNA, Satellite * MeSH

The W chromosome of most lepidopteran species represents the largest heterochromatin entity in the female genome. Although satellite DNA is a typical component of constitutive heterochromatin, there are only a few known satellite DNAs (satDNAs) located on the W chromosome in moths and butterflies. In this study, we isolated and characterized new satDNA (PiSAT1) from microdissected W chromosomes of the Indian meal moth, Plodia interpunctella. Even though the PiSAT1 is mainly localized near the female-specific segment of the W chromosome, short arrays of this satDNA also occur on autosomes and/or the Z chromosome. Probably due to the predominant location in the non-recombining part of the genome, PiSAT1 exhibits a relatively large nucleotide variability in its monomers. However, at least a part of all predicted functional motifs is located in conserved regions. Moreover, we detected polyadenylated transcripts of PiSAT1 in all developmental stages and in both sexes (female and male larvae, pupae and adults). Our results suggest a potential structural and functional role of PiSAT1 in the P. interpunctella genome, which is consistent with accumulating evidence for the important role of satDNAs in eukaryotic genomes.

Faculty of Science University of South Bohemia Branišovská 31 37005 České Budějovice Czech Republic

Institute of Entomology Biology Centre CAS České Budějovice Czech Republic

Veterinary Research Institute Brno Czech Republic

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