In Vitro Antistaphylococcal Synergistic Effect of Isoflavone Metabolite Demethyltexasin with Amoxicillin and Oxacillin
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
28570834
DOI
10.1089/mdr.2017.0033
Knihovny.cz E-resources
- Keywords
- antibiotic resistance, antimicrobial combinatory effect, checkerboard method, β-lactam antibiotics,
- MeSH
- Amoxicillin pharmacology MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Drug Combinations MeSH
- Isoflavones pharmacology MeSH
- Amoxicillin-Potassium Clavulanate Combination pharmacology MeSH
- Humans MeSH
- Methicillin-Resistant Staphylococcus aureus drug effects growth & development MeSH
- Microbial Sensitivity Tests MeSH
- Oxacillin pharmacology MeSH
- Methicillin Resistance drug effects MeSH
- Staphylococcus aureus drug effects growth & development MeSH
- Drug Synergism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- 6,7,4'-trihydroxyisoflavone MeSH Browser
- Amoxicillin MeSH
- Anti-Bacterial Agents MeSH
- Drug Combinations MeSH
- Isoflavones MeSH
- Amoxicillin-Potassium Clavulanate Combination MeSH
- Oxacillin MeSH
Staphylococcal infections are often hard to treat due to increasing resistance, especially to β-lactams. Previous studies described the synergy between common antibiotics and isoflavonoids; however, little is yet known about the combinatory effects of antibiotics with products of human isoflavone metabolism. In this study, demethyltexasin (DT), a human body metabolite of soybean isoflavones, was evaluated for its possible antistaphylococcal combinatory effect with amoxicillin and oxacillin. For comparison, common therapeutically used combination of amoxicillin/clavulanic acid was tested. DT showed strong synergistic interactions against most of Staphylococcus aureus strains when combined with amoxicillin (sum of fractional inhibitory concentrations [ΣFIC] 0.257-0.461) and oxacillin (ΣFIC 0.109-0.484). When oxacillin was combined with DT, resistance to this antibiotic was overcome in many cases. Moreover, antibiotic/DT combinations were effective mainly against methicillin-resistant S. aureus (MRSA); however, the commonly used drug amoxicillin/clavulanic acid was effective only against sensitive strains. Our results indicated DT as a compound able to act synergistically with β-lactams. In addition, some combinations are effective against MRSA and decrease staphylococcal resistance. To the best of our knowledge this is the first report of the antimicrobial synergistic effects of isoflavone human body metabolite with common antibiotics. DT seems to be a possible candidate for further research focused on antistaphylococcal drug development, especially against antibiotic-resistant strains.
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