The inactivation of four micromycete species by action of non-thermal plasma was followed. Two sources of plasma were compared, namely, positive corona discharge and dielectric barrier discharge. The corona discharge appeared as suitable for fungal spore inactivation in water suspension, whereas the barrier discharge inactivated spores on the surface of cultivation agar. Cladosporium sphaerospermum was the most sensitive, being inactivated within 10 min of exposure to plasma, whereas Aspergillus oryzae displayed decrease in viable cell count only, the complete inactivation was not achieved even after 40 min of exposure. Intermediate sensitivity was found for Alternaria sp. and Byssochlamys nivea. The significant delay of growth was observed for all fungi after exposure to sublethal dose of plasma, but we failed to express this effect quantitatively.

Department of Biochemistry and Microbiology Faculty of Food and Biochemical Technology University of Chemistry and Technology Prague 166 28 Prague Czech Republic

Department of Computing and Control Engineering Faculty of Chemical Engineering University of Chemistry and Technology Prague 166 28 Prague Czech Republic

Department of Physics and Measurements Faculty of Chemical Engineering University of Chemistry and Technology Prague 166 28 Prague Czech Republic

Department of Physics Czech Technical University Prague 166 27 Prague Czech Republic

Institute of Immunology and Microbiology 1st Faculty of Medicine Charles University 128 00 Prague Czech Republic

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