Modulation of GABA and glycine receptors in rat pyramidal hippocampal neurones by 3α5β-pregnanolone derivatives
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
29886074
DOI
10.1016/j.neuint.2018.06.002
PII: S0197-0186(18)30187-6
Knihovny.cz E-resources
- Keywords
- GABA receptor, Glycine receptor, Neurosteroid, Pregnanolone, Structure-activity relationship,
- MeSH
- Hippocampus drug effects physiology MeSH
- Rats MeSH
- Neurons drug effects physiology MeSH
- Neurotransmitter Agents chemistry pharmacology MeSH
- Organ Culture Techniques MeSH
- Rats, Wistar MeSH
- Pregnanolone chemistry pharmacology MeSH
- Pyramidal Cells MeSH
- Receptors, GABA-A physiology MeSH
- Receptors, Glycine physiology MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Neurotransmitter Agents MeSH
- Pregnanolone MeSH
- Receptors, GABA-A MeSH
- Receptors, Glycine MeSH
The ability of pregnanolone glutamate (PA-Glu), pregnanolone hemisuccinate (PA-hSuc) and pregnanolone hemipimelate (PA-hPim), neuroactive steroids with a negative modulatory effect on excitatory N-methyl-d-aspartate receptors, to influence the functional activity of inhibitory γ-aminobutyric acid and glycine receptors was estimated. The GABA- and glycine-induced chloride currents (IGABA and IGly) were measured in isolated pyramidal neurons of the rat hippocampus using the patch-clamp technique. Compound PA-Glu was found to potentiate IGABA and to inhibit IGly, while PA-hSuc and PA-hPim inhibited both IGABA and IGly. Moreover, PA-Glu, PA-hSuc, and PA-hPim had a greater effect on desensitization than on the peak amplitude of IGly. At a high concentration of glycine (500 μM), the effect of neurosteroids on the peak amplitude of IGly disappeared, and the acceleration of desensitization remained. The conversion of PA-Glu into androstane glutamate (AND-Glu), an analogue that lacks the C-17 acetyl moiety, completely eliminated the effects on these receptors. Our results indicate that the C-17 acetyl moiety is crucial for the action on IGABA and IGly. Our results indicate that the pregnanolone derivatives, in contrast to the androstane analogues, modulate IGABA and IGly at low micromolar concentrations and this family of neurosteroids can be useful for future structure-activity relationship studies of the steroid modulation of other receptor types.
References provided by Crossref.org
Corticosteroids as Selective and Effective Modulators of Glycine Receptors
Epipregnanolone as a Positive Modulator of GABAA Receptor in Rat Cerebellar and Hippocampus Neurons
